Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay
The Pacific oyster represents an important part of the aquaculture production, with 555 913 tons produced in 2013. France is the fourth world producer of oysters and Crassostrea gigas is the principal cultivated species in the Arcachon bay. However, in recent years, problems of recruitment, capture...
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Other Authors: | , , , , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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HAL CCSD
2016
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Subjects: | |
Online Access: | https://theses.hal.science/tel-01306578 https://theses.hal.science/tel-01306578/document https://theses.hal.science/tel-01306578/file/GAMAIN_PERRINE_2016_CORR.pdf |
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ftunivnantes:oai:HAL:tel-01306578v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
French |
topic |
Crassostrea gigas Embryo-larval test Pollutants Climate change Salinity Comportemental analysis Test embryo-larvaire Polluants Changement climatique Température Salinité Analyse comportementale [SDE.MCG]Environmental Sciences/Global Changes [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BDD]Life Sciences [q-bio]/Development Biology |
spellingShingle |
Crassostrea gigas Embryo-larval test Pollutants Climate change Salinity Comportemental analysis Test embryo-larvaire Polluants Changement climatique Température Salinité Analyse comportementale [SDE.MCG]Environmental Sciences/Global Changes [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BDD]Life Sciences [q-bio]/Development Biology Gamain, Perrine Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay |
topic_facet |
Crassostrea gigas Embryo-larval test Pollutants Climate change Salinity Comportemental analysis Test embryo-larvaire Polluants Changement climatique Température Salinité Analyse comportementale [SDE.MCG]Environmental Sciences/Global Changes [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BDD]Life Sciences [q-bio]/Development Biology |
description |
The Pacific oyster represents an important part of the aquaculture production, with 555 913 tons produced in 2013. France is the fourth world producer of oysters and Crassostrea gigas is the principal cultivated species in the Arcachon bay. However, in recent years, problems of recruitment, capture and mortality of juvenile or adult oysters occurred. The increased frequency of these events can be indicative of changes in the global quality of the Arcachon bay. In this context of crisis, this work has been focused on the impact of two pollutants, copper and S-metolachlor, mostly found in the waters of the Arcachon bay, on D-larvae embryo-larval development of the Pacific oyster (24h post -fecundation). In a context of global climate change, a multifactorial approach was adopted to study the combined effects of pollutants and increase temperature or salinity changes that could affect the development and survival of embryos and larvae during reproduction season. First, the embryo-toxic effects of copper or S-metolachlor coupled to salinity or different temperatures were studied with oysters from hatchery. For this, the embryo-larval test was used, and its application limits specified. Secondly, the effects of pollutants combined or not to environmental temperature and salinity were analyzed on embryos from wild or cultivated oysters harvested directly on different sites of the Arcachon bay. The percentage of abnormalities and the differential expression levels of target genes were determined in larvae while the Cu and S-metolachlor bioaccumulation and the percentage of gonadal occupation were measured in genitors. These results were then compared to determine the ability of oyster from hatchery to represent an interesting alternative model to indigenous oysters. With the objective to study the impacts of climate change, somewhat more extreme conditions were tested, namely temperatures of 26 ° C, lower salinity of 24 u.s.i and higher concentrations of pollutants compared to current conditions in the lagoon. A ... |
author2 |
Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Université de Bordeaux Patrice Gonzalez |
format |
Doctoral or Postdoctoral Thesis |
author |
Gamain, Perrine |
author_facet |
Gamain, Perrine |
author_sort |
Gamain, Perrine |
title |
Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay |
title_short |
Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay |
title_full |
Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay |
title_fullStr |
Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay |
title_full_unstemmed |
Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay |
title_sort |
impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster crassostrea gigas in the arcachon bay |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://theses.hal.science/tel-01306578 https://theses.hal.science/tel-01306578/document https://theses.hal.science/tel-01306578/file/GAMAIN_PERRINE_2016_CORR.pdf |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
https://theses.hal.science/tel-01306578 Milieux et Changements globaux. Université de Bordeaux, 2016. Français. ⟨NNT : 2016BORD0011⟩ |
op_relation |
NNT: 2016BORD0011 tel-01306578 https://theses.hal.science/tel-01306578 https://theses.hal.science/tel-01306578/document https://theses.hal.science/tel-01306578/file/GAMAIN_PERRINE_2016_CORR.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766393511801782272 |
spelling |
ftunivnantes:oai:HAL:tel-01306578v1 2023-05-15T15:57:49+02:00 Impact of pollutants and climate change stressors on reproduction capacity and embryo- larval development of the oyster Crassostrea gigas in the Arcachon bay Impact des polluants et du changement climatique sur les capacités de reproduction et le développement embryo larvaire de l’huître creuse Crassostrea gigas dans le Bassin d’Arcachon Gamain, Perrine Environnements et Paléoenvironnements OCéaniques (EPOC) Observatoire aquitain des sciences de l'univers (OASU) Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS) Université de Bordeaux Patrice Gonzalez 2016-02-15 https://theses.hal.science/tel-01306578 https://theses.hal.science/tel-01306578/document https://theses.hal.science/tel-01306578/file/GAMAIN_PERRINE_2016_CORR.pdf fr fre HAL CCSD NNT: 2016BORD0011 tel-01306578 https://theses.hal.science/tel-01306578 https://theses.hal.science/tel-01306578/document https://theses.hal.science/tel-01306578/file/GAMAIN_PERRINE_2016_CORR.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-01306578 Milieux et Changements globaux. Université de Bordeaux, 2016. Français. ⟨NNT : 2016BORD0011⟩ Crassostrea gigas Embryo-larval test Pollutants Climate change Salinity Comportemental analysis Test embryo-larvaire Polluants Changement climatique Température Salinité Analyse comportementale [SDE.MCG]Environmental Sciences/Global Changes [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology [SDV.BDD]Life Sciences [q-bio]/Development Biology info:eu-repo/semantics/doctoralThesis Theses 2016 ftunivnantes 2023-02-08T10:14:29Z The Pacific oyster represents an important part of the aquaculture production, with 555 913 tons produced in 2013. France is the fourth world producer of oysters and Crassostrea gigas is the principal cultivated species in the Arcachon bay. However, in recent years, problems of recruitment, capture and mortality of juvenile or adult oysters occurred. The increased frequency of these events can be indicative of changes in the global quality of the Arcachon bay. In this context of crisis, this work has been focused on the impact of two pollutants, copper and S-metolachlor, mostly found in the waters of the Arcachon bay, on D-larvae embryo-larval development of the Pacific oyster (24h post -fecundation). In a context of global climate change, a multifactorial approach was adopted to study the combined effects of pollutants and increase temperature or salinity changes that could affect the development and survival of embryos and larvae during reproduction season. First, the embryo-toxic effects of copper or S-metolachlor coupled to salinity or different temperatures were studied with oysters from hatchery. For this, the embryo-larval test was used, and its application limits specified. Secondly, the effects of pollutants combined or not to environmental temperature and salinity were analyzed on embryos from wild or cultivated oysters harvested directly on different sites of the Arcachon bay. The percentage of abnormalities and the differential expression levels of target genes were determined in larvae while the Cu and S-metolachlor bioaccumulation and the percentage of gonadal occupation were measured in genitors. These results were then compared to determine the ability of oyster from hatchery to represent an interesting alternative model to indigenous oysters. With the objective to study the impacts of climate change, somewhat more extreme conditions were tested, namely temperatures of 26 ° C, lower salinity of 24 u.s.i and higher concentrations of pollutants compared to current conditions in the lagoon. A ... Doctoral or Postdoctoral Thesis Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific |