Evidence of the presence of Seoul virus in Cambodia

• Published in: Microbes and Infection
• Main Authors: Reynes, Jean-Marc, Soares, Jean-Louis, Hüe, Thomas, Bouloy, Michele, Sun, Sot, Kruy, Sun, Flye Sainte Marie, François, Zeller, Hervé
• Other Authors: Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Génétique Moléculaire des Bunyavirus, Institut Pasteur Paris (IP), Ministère de l’Agriculture des Forêts et de la Pêche Phnom Penh, Centre national de Référence des Fièvres hémorragiques virales - National Reference Center Haemorrhagic Fevers (CNR FHV - UBIVE), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur Paris (IP)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2003
• Subjects: Seoul virus; Hantavirus; Cambodia; Rodent; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology; [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie; Norway; Rattus rattus
• Online Access: https://hal-pasteur.archives-ouvertes.fr/pasteur-01836869; https://hal-pasteur.archives-ouvertes.fr/pasteur-01836869/document; https://hal-pasteur.archives-ouvertes.fr/pasteur-01836869/file/2003-MI-SEOV%20KHM.pdf; https://doi.org/10.1016/S1286-4579(03)00149-7

International audience A study was conducted in agricultural and urban areas in Cambodia to assess the presence of hantaviruses in rodent populations. In 1998, rodents were trapped in two villages and in Phnom Penh city around market places and a rubbish dump. IgG antibodies to Hantaan virus were detected in 54 (8.2%) rodents among 660 tested: 6.4% (13/203) among roof rats (Rattus rattus), 20.9% (39/187) among Norway rats (R. norvegicus), 16.7% (2/12) among unidentified Rattus species and none in 183 Polynesian rats (R. exulans) or in 75 bandicoot rats (Bandicota sp.). The presence of the viral genome was detected by a reverse transcription-PCR amplifying part of the sequence coding for the nucleoprotein in the S segment, in 87% of the seropositive rodents. Thirty-one representative cDNAs were sequenced. Phylogenetic studies of the sequences indicated a close relationship with Seoul virus. However, the Cambodian Seoul virus sequences clustered within two different phylogenetic lineages, one associated with R. rattus and the other with R. norvegicus.