Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar
International audience Background: Rickettsiae are obligate intracellular bacteria responsible for many febrile syndromes around the world,including in sub-Saharan Africa. Vectors of these pathogens include ticks, lice, mites and fleas. In order to assessexposure to flea-associated Rickettsia specie...
Published in: | Parasites & Vectors |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2017
|
Subjects: | |
Online Access: | https://hal-riip.archives-ouvertes.fr/pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154/document https://hal-riip.archives-ouvertes.fr/pasteur-01652154/file/molecular_serological%20evidence%20of%20flea-associated%20typhus_Rakotonanaharyetal.pdf https://doi.org/10.1186/s13071-017-2061-4 |
id |
ftunivnantes:oai:HAL:pasteur-01652154v1 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Fleas Madagascar Murine typhus Prevalence Rickettsioses Rickettsia Flea-borne spotted fever MESH: Adult MESH: Animals MESH: Rats MESH: Rickettsia MESH: Rocky Mountain Spotted Fever MESH: Rodent Diseases MESH: Shrews MESH: Siphonaptera MESH: Typhus Epidemic Louse-Borne MESH: Young Adult MESH: Antibodies Bacterial MESH: Female MESH: Humans MESH: Insect Vectors MESH: Madagascar MESH: Male MESH: Middle Aged MESH: Phylogeny [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
spellingShingle |
Fleas Madagascar Murine typhus Prevalence Rickettsioses Rickettsia Flea-borne spotted fever MESH: Adult MESH: Animals MESH: Rats MESH: Rickettsia MESH: Rocky Mountain Spotted Fever MESH: Rodent Diseases MESH: Shrews MESH: Siphonaptera MESH: Typhus Epidemic Louse-Borne MESH: Young Adult MESH: Antibodies Bacterial MESH: Female MESH: Humans MESH: Insect Vectors MESH: Madagascar MESH: Male MESH: Middle Aged MESH: Phylogeny [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Rakotonanahary, Rado, Harrison, Alan Maina, Alice, Jiang, Ju Richards, Allen, Rajerison, Minoarisoa Telfer, Sandra Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar |
topic_facet |
Fleas Madagascar Murine typhus Prevalence Rickettsioses Rickettsia Flea-borne spotted fever MESH: Adult MESH: Animals MESH: Rats MESH: Rickettsia MESH: Rocky Mountain Spotted Fever MESH: Rodent Diseases MESH: Shrews MESH: Siphonaptera MESH: Typhus Epidemic Louse-Borne MESH: Young Adult MESH: Antibodies Bacterial MESH: Female MESH: Humans MESH: Insect Vectors MESH: Madagascar MESH: Male MESH: Middle Aged MESH: Phylogeny [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie |
description |
International audience Background: Rickettsiae are obligate intracellular bacteria responsible for many febrile syndromes around the world,including in sub-Saharan Africa. Vectors of these pathogens include ticks, lice, mites and fleas. In order to assessexposure to flea-associated Rickettsia species in Madagascar, human and small mammal samples from an urbanand a rural area, and their associated fleas were tested.Results: Anti-typhus group (TGR)- and anti-spotted fever group rickettsiae (SFGR)-specific IgG were detected in24 (39%) and 21 (34%) of 62 human serum samples, respectively, using indirect ELISAs, with six individuals seropositivefor both. Only two (2%) Rattus rattus out of 86 small mammals presented antibodies against TGR. Out of 117fleas collected from small mammals, Rickettsia typhi, a TGR, was detected in 26 Xenopsylla cheopis (24%) collected fromrodents of an urban area (n = 107), while two of these urban X. cheopis (2%) were positive for Rickettsia felis, a SFGR. R.felis DNA was also detected in eight (31%) out of 26 Pulex irritans fleas.Conclusions: The general population in Madagascar are exposed to rickettsiae, and two flea-associated Rickettsiapathogens, R. typhi and R. felis, are present near or in homes. Although our results are from a single district, theydemonstrate that rickettsiae should be considered as potential agents of undifferentiated fever in Madagascar. |
author2 |
Unité Peste - Plague Unit Antananarivo, Madagascar Institut Pasteur de Madagascar Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Université d'Antananarivo Institute of Biological, Environmental and Rural Sciences (IBERS) Biotechnology and Biological Sciences Research Council (BBSRC)-Aberystwyth University Viral and Rickettsial Diseases Department U.S. Naval Medical Research This research was supported by the Wellcome Trust (RCDF and Senior Fellowship to ST, #081705 and #095171), the Institut Pasteur de Madagascar, and the Global Emerging Infections Surveillance and Response System, a Division of the Armed Forces Health Surveillance Center 847705.82000.25GB.A0074 |
format |
Article in Journal/Newspaper |
author |
Rakotonanahary, Rado, Harrison, Alan Maina, Alice, Jiang, Ju Richards, Allen, Rajerison, Minoarisoa Telfer, Sandra |
author_facet |
Rakotonanahary, Rado, Harrison, Alan Maina, Alice, Jiang, Ju Richards, Allen, Rajerison, Minoarisoa Telfer, Sandra |
author_sort |
Rakotonanahary, Rado, |
title |
Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar |
title_short |
Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar |
title_full |
Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar |
title_fullStr |
Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar |
title_full_unstemmed |
Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar |
title_sort |
molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in madagascar |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal-riip.archives-ouvertes.fr/pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154/document https://hal-riip.archives-ouvertes.fr/pasteur-01652154/file/molecular_serological%20evidence%20of%20flea-associated%20typhus_Rakotonanaharyetal.pdf https://doi.org/10.1186/s13071-017-2061-4 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 1756-3305 EISSN: 1756-3305 Parasites & Vectors https://hal-riip.archives-ouvertes.fr/pasteur-01652154 Parasites & Vectors, 2017, 10 (1), pp.125. ⟨10.1186/s13071-017-2061-4⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-017-2061-4 info:eu-repo/semantics/altIdentifier/pmid/28259176 pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154/document https://hal-riip.archives-ouvertes.fr/pasteur-01652154/file/molecular_serological%20evidence%20of%20flea-associated%20typhus_Rakotonanaharyetal.pdf doi:10.1186/s13071-017-2061-4 PUBMED: 28259176 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s13071-017-2061-4 |
container_title |
Parasites & Vectors |
container_volume |
10 |
container_issue |
1 |
_version_ |
1766177182420303872 |
spelling |
ftunivnantes:oai:HAL:pasteur-01652154v1 2023-05-15T18:05:41+02:00 Molecular and serological evidence of flea-associated typhus group and spotted fever group rickettsial infections in Madagascar Rakotonanahary, Rado, Harrison, Alan Maina, Alice, Jiang, Ju Richards, Allen, Rajerison, Minoarisoa Telfer, Sandra Unité Peste - Plague Unit Antananarivo, Madagascar Institut Pasteur de Madagascar Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) Université d'Antananarivo Institute of Biological, Environmental and Rural Sciences (IBERS) Biotechnology and Biological Sciences Research Council (BBSRC)-Aberystwyth University Viral and Rickettsial Diseases Department U.S. Naval Medical Research This research was supported by the Wellcome Trust (RCDF and Senior Fellowship to ST, #081705 and #095171), the Institut Pasteur de Madagascar, and the Global Emerging Infections Surveillance and Response System, a Division of the Armed Forces Health Surveillance Center 847705.82000.25GB.A0074 2017-03-04 https://hal-riip.archives-ouvertes.fr/pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154/document https://hal-riip.archives-ouvertes.fr/pasteur-01652154/file/molecular_serological%20evidence%20of%20flea-associated%20typhus_Rakotonanaharyetal.pdf https://doi.org/10.1186/s13071-017-2061-4 en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-017-2061-4 info:eu-repo/semantics/altIdentifier/pmid/28259176 pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154 https://hal-riip.archives-ouvertes.fr/pasteur-01652154/document https://hal-riip.archives-ouvertes.fr/pasteur-01652154/file/molecular_serological%20evidence%20of%20flea-associated%20typhus_Rakotonanaharyetal.pdf doi:10.1186/s13071-017-2061-4 PUBMED: 28259176 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1756-3305 EISSN: 1756-3305 Parasites & Vectors https://hal-riip.archives-ouvertes.fr/pasteur-01652154 Parasites & Vectors, 2017, 10 (1), pp.125. ⟨10.1186/s13071-017-2061-4⟩ Fleas Madagascar Murine typhus Prevalence Rickettsioses Rickettsia Flea-borne spotted fever MESH: Adult MESH: Animals MESH: Rats MESH: Rickettsia MESH: Rocky Mountain Spotted Fever MESH: Rodent Diseases MESH: Shrews MESH: Siphonaptera MESH: Typhus Epidemic Louse-Borne MESH: Young Adult MESH: Antibodies Bacterial MESH: Female MESH: Humans MESH: Insect Vectors MESH: Madagascar MESH: Male MESH: Middle Aged MESH: Phylogeny [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie info:eu-repo/semantics/article Journal articles 2017 ftunivnantes https://doi.org/10.1186/s13071-017-2061-4 2023-01-11T00:28:58Z International audience Background: Rickettsiae are obligate intracellular bacteria responsible for many febrile syndromes around the world,including in sub-Saharan Africa. Vectors of these pathogens include ticks, lice, mites and fleas. In order to assessexposure to flea-associated Rickettsia species in Madagascar, human and small mammal samples from an urbanand a rural area, and their associated fleas were tested.Results: Anti-typhus group (TGR)- and anti-spotted fever group rickettsiae (SFGR)-specific IgG were detected in24 (39%) and 21 (34%) of 62 human serum samples, respectively, using indirect ELISAs, with six individuals seropositivefor both. Only two (2%) Rattus rattus out of 86 small mammals presented antibodies against TGR. Out of 117fleas collected from small mammals, Rickettsia typhi, a TGR, was detected in 26 Xenopsylla cheopis (24%) collected fromrodents of an urban area (n = 107), while two of these urban X. cheopis (2%) were positive for Rickettsia felis, a SFGR. R.felis DNA was also detected in eight (31%) out of 26 Pulex irritans fleas.Conclusions: The general population in Madagascar are exposed to rickettsiae, and two flea-associated Rickettsiapathogens, R. typhi and R. felis, are present near or in homes. Although our results are from a single district, theydemonstrate that rickettsiae should be considered as potential agents of undifferentiated fever in Madagascar. Article in Journal/Newspaper Rattus rattus Université de Nantes: HAL-UNIV-NANTES Parasites & Vectors 10 1 |