Molecular evolution and antigenic variation of European brown hare syndrome virus (EBHSV)

• Published in: Virology
• Main Authors: Lopes, Ana, M., Capucci, Lorenzo, Gavier-Widén, Dolores, Le Gall-Reculé, Ghislaine, Brocchi, Emiliana, Barbieri, Ilaria, Quéméner, Agnès, Le Pendu, Jacques, Geoghegan, Jemma, L, Holmes, Edward, C, Esteves, Pedro, Abrantes, Joana
• Other Authors: Departamento de Biologia Porto, Portugal, Faculdade de Ciências da Universidade do Porto (FCUP), Universidade do Porto = University of Porto-Universidade do Porto = University of Porto, Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Université d'Angers (UA)-Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), CIBIO/UP-Centro de Investigação em Biodiversidade e Recursos Genéticos Vairão, Portugal, Universidade do Porto = University of Porto, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" (IZSLER), Department Pathology and Wildlife Disease, Natl Vet Inst SVA, Section of Immunology Uppsala, Sweden (Department of Biomedical Sciences and Veterinary Public Health), Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU)-Veterinary and Animal Science Center Uppsala, Sweden, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université européenne de Bretagne - European University of Brittany (UEB), Sydney Emerging Infectious Diseases and Biosecurity Institute (SEIB), The University of Sydney, CIBIO-UP, Centro de Investigacao em Tecnologias de Saude, CESPU, Centro de Investigacao em Biodiverdidade e Recursos Geneticos (CIBIO-UP), FCT-ANR/BIA-BIC/0043/2012, This work was partially supported by FEDER, Portugal (Fundo Europeu de Desenvolvimento Regional) funds through the Programa Operacional Factores de Competitividade (COMPETE program) and Portuguese national funds through the FCT, Portugal(Fundação para a Ciência e a Tecnologia; FCT-ANR/BIA-BIC/0043/2012 research project), by Programa Operacional Fatores de Competitividade (POFC) – Quadro de Referência Estratégico Nacional (QREN) funds from the European Social Fund and Portuguese Ministério da Educação e Ciência and by the Wildtech Project (EU 7th Framework Program for Research and Technological Development, Grant Agreement No. 222633). Also, project “Genomics Applied to Genetic Resources,” co-financed by North Portugal Regional Operational Programme 2007/2013 (ON.2–O Novo Norte), under the National Strategic Reference Framework, through the European Regional Development Fund, supported this work. The French molecular study was supported by grants from the National Hunting and Wildlife Agency (Office National de la Chasse et de la Faune Sauvage, ONCFS, Agreements 2006/08/6171 and 2010/14/6171). The doctoral fellowship of AML (ref.: SFRH/BD/78738/2011) is supported by FCT. ECH is supported by an NHMRC Australia Fellowship and by ARC Grant DP140103362. JA is supported by an FCT Investigator Grant (ref.: IF/01396/2013)., European Project: 222633,EC:FP7:KBBE,FP7-KBBE-2007-2A,WILDTECH(2009)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2014
• Subjects: Evolution; European brown hare syndrome virus (EBHSV); Antigenic variation; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology; Lepus timidus
• Online Access: https://inserm.hal.science/inserm-02441033; https://inserm.hal.science/inserm-02441033/document; https://inserm.hal.science/inserm-02441033/file/2014LopesVirology.pdf; https://doi.org/10.1016/j.virol.2014.08.002

International audience European brown hare syndrome virus(EBHSV) is the aetiological agent of European brown hare syndrome (EBHS), a disease affecting Lepus europaeus and Lepus timidus first diagnosed in Sweden in 1980. To characterize EBHSV evolution we studied hare samples collected in Sweden between 1982 and2008. Our molecular clock dating is compatible with EBHSV emergence in the 1970s. Phylogenetic analysis revealed two lineages: Group A persisted until 1989 when it apparently suffered extinction; Group B emerged in the mid-1980s and contains the most recent strains. Antigenic differences exist between groups, with loss of reactivity of some MAbs overtime, which are associated with aminoacid substitutions in recognized epitopes. A role for immune selection is also supported by the presence of positively selected codons in exposed regions of the capsid. Hence, EBHSV evolution is characterized by replacement of Group A by Group B viruses, suggesting that the latter possess a selective advantage.