Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus)
International audience This work describes some consequences of paternal germ cell DNA damage on the reproduction success in two fish species. Male brown trout (n = 31) and male Arctic charr (n = 28) were exposed to the model genotoxicant MMS at the end of spermatogenesis to generate a significant D...
Published in: | Aquatic Toxicology |
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Online Access: | https://hal.archives-ouvertes.fr/halsde-00578879 https://doi.org/10.1016/j.aquatox.2010.11.017 |
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ftunivnantes:oai:HAL:halsde-00578879v1 2023-05-15T14:30:09+02:00 Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) Devaux, Alain Fiat, Luc Gillet, Christian Bony, Sylvie Équipe 5 - Impacts des Polluants sur les Écosystèmes Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ) 2011 https://hal.archives-ouvertes.fr/halsde-00578879 https://doi.org/10.1016/j.aquatox.2010.11.017 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2010.11.017 halsde-00578879 https://hal.archives-ouvertes.fr/halsde-00578879 doi:10.1016/j.aquatox.2010.11.017 PRODINRA: 47453 WOS: 000287423900013 ISSN: 0166-445X Aquatic Toxicology https://hal.archives-ouvertes.fr/halsde-00578879 Aquatic Toxicology, Elsevier, 2011, 101 (2), pp.405-411. ⟨10.1016/j.aquatox.2010.11.017⟩ Reproduction Genotoxicity Sperm Embryo Development Fish [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2011 ftunivnantes https://doi.org/10.1016/j.aquatox.2010.11.017 2022-10-04T22:58:21Z International audience This work describes some consequences of paternal germ cell DNA damage on the reproduction success in two fish species. Male brown trout (n = 31) and male Arctic charr (n = 28) were exposed to the model genotoxicant MMS at the end of spermatogenesis to generate a significant DNA damage level in mature spermatozoa (28% and 25% tail DNA in trout and charr sperm, respectively, evaluated through the comet assay). Sperm from each MMS exposed and control fish was then used to fertilize in vitro an aliquot of a single pool of eggs collected from 4 unexposed females for each species. Each batch of fertilized eggs was monitored individually in the hatchery to follow embryonic and larval abnormalities during the fry development. Paternal exposure did not influence fertilization rate or survival rate at hatching in either species. However, MMS paternal treatment resulted in a large array of morphological abnormalities during embryonic and larval development. At the eyed stage, malformations exhibited a 8 fold increase in trout and a 2 fold increase in charr for larvae stemming from MMS treated males as compared with controls. At the end of yolk sac resorption, an increase in the gross morphological abnormality incidence was found in trout larvae originating from MMS exposed males (2.10% vs. 0.93% in control, p < 0.05). When looking more in detail at bony structures after Alizarin red S staining, a 20% incidence of skeletal defects was recorded at the swimming stage. A positive correlation was found between the paternal sperm DNA damage level and the skeletal abnormality incidence of its progeny. During the next 2 months of development, mortality in trout originating from DNA damaged sperm was 3 times higher than in control. After one year, no effect of paternal treatment was found on growth traits (length and weight) but the gross morphological abnormality incidence was still very high in the treated group (27% malformation incidence vs. 0.5% in control). These results demonstrate ecologically ... Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Université de Nantes: HAL-UNIV-NANTES Arctic Aquatic Toxicology 101 2 405 411 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Reproduction Genotoxicity Sperm Embryo Development Fish [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Reproduction Genotoxicity Sperm Embryo Development Fish [SDE.BE]Environmental Sciences/Biodiversity and Ecology Devaux, Alain Fiat, Luc Gillet, Christian Bony, Sylvie Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) |
topic_facet |
Reproduction Genotoxicity Sperm Embryo Development Fish [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience This work describes some consequences of paternal germ cell DNA damage on the reproduction success in two fish species. Male brown trout (n = 31) and male Arctic charr (n = 28) were exposed to the model genotoxicant MMS at the end of spermatogenesis to generate a significant DNA damage level in mature spermatozoa (28% and 25% tail DNA in trout and charr sperm, respectively, evaluated through the comet assay). Sperm from each MMS exposed and control fish was then used to fertilize in vitro an aliquot of a single pool of eggs collected from 4 unexposed females for each species. Each batch of fertilized eggs was monitored individually in the hatchery to follow embryonic and larval abnormalities during the fry development. Paternal exposure did not influence fertilization rate or survival rate at hatching in either species. However, MMS paternal treatment resulted in a large array of morphological abnormalities during embryonic and larval development. At the eyed stage, malformations exhibited a 8 fold increase in trout and a 2 fold increase in charr for larvae stemming from MMS treated males as compared with controls. At the end of yolk sac resorption, an increase in the gross morphological abnormality incidence was found in trout larvae originating from MMS exposed males (2.10% vs. 0.93% in control, p < 0.05). When looking more in detail at bony structures after Alizarin red S staining, a 20% incidence of skeletal defects was recorded at the swimming stage. A positive correlation was found between the paternal sperm DNA damage level and the skeletal abnormality incidence of its progeny. During the next 2 months of development, mortality in trout originating from DNA damaged sperm was 3 times higher than in control. After one year, no effect of paternal treatment was found on growth traits (length and weight) but the gross morphological abnormality incidence was still very high in the treated group (27% malformation incidence vs. 0.5% in control). These results demonstrate ecologically ... |
author2 |
Équipe 5 - Impacts des Polluants sur les Écosystèmes Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS) Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ) |
format |
Article in Journal/Newspaper |
author |
Devaux, Alain Fiat, Luc Gillet, Christian Bony, Sylvie |
author_facet |
Devaux, Alain Fiat, Luc Gillet, Christian Bony, Sylvie |
author_sort |
Devaux, Alain |
title |
Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) |
title_short |
Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) |
title_full |
Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) |
title_fullStr |
Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) |
title_full_unstemmed |
Reproduction impairment following paternal genotoxin exposure in brown trout (Salmo trutta) and Arctic charr (Salvelinus alpinus) |
title_sort |
reproduction impairment following paternal genotoxin exposure in brown trout (salmo trutta) and arctic charr (salvelinus alpinus) |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.archives-ouvertes.fr/halsde-00578879 https://doi.org/10.1016/j.aquatox.2010.11.017 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.archives-ouvertes.fr/halsde-00578879 Aquatic Toxicology, Elsevier, 2011, 101 (2), pp.405-411. ⟨10.1016/j.aquatox.2010.11.017⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2010.11.017 halsde-00578879 https://hal.archives-ouvertes.fr/halsde-00578879 doi:10.1016/j.aquatox.2010.11.017 PRODINRA: 47453 WOS: 000287423900013 |
op_doi |
https://doi.org/10.1016/j.aquatox.2010.11.017 |
container_title |
Aquatic Toxicology |
container_volume |
101 |
container_issue |
2 |
container_start_page |
405 |
op_container_end_page |
411 |
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1766304051810533376 |