Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae)
Times Cited: 15 International audience Saxifraga opdalensis was described from Oppdal in southern Norway and hypothesized to have originated as the hybrid S. cernua x rivularis or to have been derived From a S. cernua-like progenitor. We tested these alternative hypotheses using uni- and biparentall...
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ftunivnantes:oai:HAL:halsde-00280565v1 2023-05-15T18:15:09+02:00 Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) Steen, S. W. Gielly, L. Taberlet, P. Brochmann, C. Natural History Museum Oslo University of Oslo (UiO) Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) 2000 https://hal.science/halsde-00280565 https://doi.org/10.1111/j.1095-8339.2000.tb01211.x en eng HAL CCSD Linnean Society of London info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1095-8339.2000.tb01211.x halsde-00280565 https://hal.science/halsde-00280565 doi:10.1111/j.1095-8339.2000.tb01211.x ISSN: 0024-4074 EISSN: 1095-8339 Botanical Journal of the Linnean Society https://hal.science/halsde-00280565 Botanical Journal of the Linnean Society, 2000, 132 (2), pp.153-164. ⟨10.1111/j.1095-8339.2000.tb01211.x⟩ cpDNA PCR-RFLP RAPD Scandinavia Svalbard universal primers [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment info:eu-repo/semantics/article Journal articles 2000 ftunivnantes https://doi.org/10.1111/j.1095-8339.2000.tb01211.x 2023-02-08T05:51:46Z Times Cited: 15 International audience Saxifraga opdalensis was described from Oppdal in southern Norway and hypothesized to have originated as the hybrid S. cernua x rivularis or to have been derived From a S. cernua-like progenitor. We tested these alternative hypotheses using uni- and biparentally inherited molecular markers observed in S. opdalensis and its putative parental species at the type locality: PCR-RFLPs (restriction fragment length polymorphisms in amplified fragments of chloroplast DNA; cpDNA), sequences of the cpDNA intron tmL and the spacer tmL-tmF, and RAPDs (random amplified polymorphic DNAs). The data provided unambiguous support for the hybrid hypothesis. The cpDNA analyses distinguished two well-differentiated chloroplast genomes, one in S. opdalensis and S. rivularis, and another in S. cernua. The majority of the RAPD markers showed distinct additivity in S. opdalensis relative to its postulated parental species. Thus, S. opdalensis has probably originated from a hybrid with S. rivularis as the maternal parent and S. cernua as the paternal parent. We also included S. svalbardensis in the present study because previous molecular analyses of Svalbard material have shown that this species had probably also originated as a hybrid between S. cernua and S. rivularis. The chloroplast genome of S, svalbardensis was identical to that of S. opdalensis, but the two species differed in many RAPD markers. Although these two endemics probably have been derived from the same hybrid combination, they are morphologically and genetically distinct and should be referred. to separate species. Differences between such independently originated hybrid taxa may result from intraspecific variation in their parental taxa. Saxifraga cernua comprises, for example, several highly divergent evolutionary lineages. (C) 2000 The Linnean Society of London. Article in Journal/Newspaper Saxifraga cernua Svalbard Université de Nantes: HAL-UNIV-NANTES Norway Svalbard Botanical Journal of the Linnean Society 132 2 153 164 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
cpDNA PCR-RFLP RAPD Scandinavia Svalbard universal primers [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
spellingShingle |
cpDNA PCR-RFLP RAPD Scandinavia Svalbard universal primers [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment Steen, S. W. Gielly, L. Taberlet, P. Brochmann, C. Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) |
topic_facet |
cpDNA PCR-RFLP RAPD Scandinavia Svalbard universal primers [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
description |
Times Cited: 15 International audience Saxifraga opdalensis was described from Oppdal in southern Norway and hypothesized to have originated as the hybrid S. cernua x rivularis or to have been derived From a S. cernua-like progenitor. We tested these alternative hypotheses using uni- and biparentally inherited molecular markers observed in S. opdalensis and its putative parental species at the type locality: PCR-RFLPs (restriction fragment length polymorphisms in amplified fragments of chloroplast DNA; cpDNA), sequences of the cpDNA intron tmL and the spacer tmL-tmF, and RAPDs (random amplified polymorphic DNAs). The data provided unambiguous support for the hybrid hypothesis. The cpDNA analyses distinguished two well-differentiated chloroplast genomes, one in S. opdalensis and S. rivularis, and another in S. cernua. The majority of the RAPD markers showed distinct additivity in S. opdalensis relative to its postulated parental species. Thus, S. opdalensis has probably originated from a hybrid with S. rivularis as the maternal parent and S. cernua as the paternal parent. We also included S. svalbardensis in the present study because previous molecular analyses of Svalbard material have shown that this species had probably also originated as a hybrid between S. cernua and S. rivularis. The chloroplast genome of S, svalbardensis was identical to that of S. opdalensis, but the two species differed in many RAPD markers. Although these two endemics probably have been derived from the same hybrid combination, they are morphologically and genetically distinct and should be referred. to separate species. Differences between such independently originated hybrid taxa may result from intraspecific variation in their parental taxa. Saxifraga cernua comprises, for example, several highly divergent evolutionary lineages. (C) 2000 The Linnean Society of London. |
author2 |
Natural History Museum Oslo University of Oslo (UiO) Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Steen, S. W. Gielly, L. Taberlet, P. Brochmann, C. |
author_facet |
Steen, S. W. Gielly, L. Taberlet, P. Brochmann, C. |
author_sort |
Steen, S. W. |
title |
Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) |
title_short |
Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) |
title_full |
Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) |
title_fullStr |
Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) |
title_full_unstemmed |
Same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics Saxifraga opdalensis and S-svalbardensis (Saxifragaceae) |
title_sort |
same parental species, but different taxa: molecular evidence for hybrid origins of the rare endemics saxifraga opdalensis and s-svalbardensis (saxifragaceae) |
publisher |
HAL CCSD |
publishDate |
2000 |
url |
https://hal.science/halsde-00280565 https://doi.org/10.1111/j.1095-8339.2000.tb01211.x |
geographic |
Norway Svalbard |
geographic_facet |
Norway Svalbard |
genre |
Saxifraga cernua Svalbard |
genre_facet |
Saxifraga cernua Svalbard |
op_source |
ISSN: 0024-4074 EISSN: 1095-8339 Botanical Journal of the Linnean Society https://hal.science/halsde-00280565 Botanical Journal of the Linnean Society, 2000, 132 (2), pp.153-164. ⟨10.1111/j.1095-8339.2000.tb01211.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1095-8339.2000.tb01211.x halsde-00280565 https://hal.science/halsde-00280565 doi:10.1111/j.1095-8339.2000.tb01211.x |
op_doi |
https://doi.org/10.1111/j.1095-8339.2000.tb01211.x |
container_title |
Botanical Journal of the Linnean Society |
container_volume |
132 |
container_issue |
2 |
container_start_page |
153 |
op_container_end_page |
164 |
_version_ |
1766188214305947648 |