Molecular genetics and field observations to assist Brown Bear population in the Pyrenees
International audience The remnant Brown Bear (Ursus arctos) population in the Pyrenees mountains on the border between France and Spain is among the most threatened in Europe. To obtain critical information for the management of this Brown Bear population, the French Ministry of the Environment ini...
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ftunivnantes:oai:HAL:halsde-00280561v1 2023-05-15T18:42:02+02:00 Molecular genetics and field observations to assist Brown Bear population in the Pyrenees Bouvet, J. Taberlet, P. Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) 2001 https://hal.science/halsde-00280561 en eng HAL CCSD Societe Zoologique De France halsde-00280561 https://hal.science/halsde-00280561 ISSN: 0037-962X Bulletin De La Societe Zoologique De France https://hal.science/halsde-00280561 Bulletin De La Societe Zoologique De France, 2001, 126 (1-2), pp.179-188 CONSERVATION GENETICS URSUS-ARCTOS [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment info:eu-repo/semantics/article Journal articles 2001 ftunivnantes 2023-02-08T05:51:46Z International audience The remnant Brown Bear (Ursus arctos) population in the Pyrenees mountains on the border between France and Spain is among the most threatened in Europe. To obtain critical information for the management of this Brown Bear population, the French Ministry of the Environment initiated a research programme in 1991 with three major goals : (i) identify the potential conservation units at the European level, (ii) develop a method to determine the sex of free-ranging bears, (iii) establish unique genetic identification of all remaining individuals using noninvasive sampling techniques. Previous studies have demonstrated the potential for hair or faeces collected in the field to provide a suitable source of DNA for genotyping and sexing free-ranging animals. Despite recent advances, noninvasive genetic sampling represents a difficult challenge as hair and faeces provide only picogram quantities of degraded template DNA. Under these limiting conditions, there are two major genotyping errors which may lead to inaccurate results : an allele of a heterozygous individual may not be detected, or PCR-generated alleles or false alleles may arise. To overcome these difficulties, a multiple-tubes approach ha been recently developed, Using this method, the DNA extract is distributed among seven tubes and amplified separately to determine the genotype with a 99% accuracy. Before the beginning of this genetic study, field data based mainly on the recording of track sizes suggested the presence of only several animals. Five unique genotypes were identified from the six polymorphic microsatellite loci The genotypic data for each sample were compared with the corresponding track size in order to obtain a minimum estimate of the population size. Because one genotype was found with two nonoverlapping track sizes, we were able to detect six individual bears. The g genotype of one bear was not observed after 1993. The population is composed of one yearling, three adult males and one adult female, This work extends ... Article in Journal/Newspaper Ursus arctos Université de Nantes: HAL-UNIV-NANTES |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
CONSERVATION GENETICS URSUS-ARCTOS [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
spellingShingle |
CONSERVATION GENETICS URSUS-ARCTOS [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment Bouvet, J. Taberlet, P. Molecular genetics and field observations to assist Brown Bear population in the Pyrenees |
topic_facet |
CONSERVATION GENETICS URSUS-ARCTOS [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
description |
International audience The remnant Brown Bear (Ursus arctos) population in the Pyrenees mountains on the border between France and Spain is among the most threatened in Europe. To obtain critical information for the management of this Brown Bear population, the French Ministry of the Environment initiated a research programme in 1991 with three major goals : (i) identify the potential conservation units at the European level, (ii) develop a method to determine the sex of free-ranging bears, (iii) establish unique genetic identification of all remaining individuals using noninvasive sampling techniques. Previous studies have demonstrated the potential for hair or faeces collected in the field to provide a suitable source of DNA for genotyping and sexing free-ranging animals. Despite recent advances, noninvasive genetic sampling represents a difficult challenge as hair and faeces provide only picogram quantities of degraded template DNA. Under these limiting conditions, there are two major genotyping errors which may lead to inaccurate results : an allele of a heterozygous individual may not be detected, or PCR-generated alleles or false alleles may arise. To overcome these difficulties, a multiple-tubes approach ha been recently developed, Using this method, the DNA extract is distributed among seven tubes and amplified separately to determine the genotype with a 99% accuracy. Before the beginning of this genetic study, field data based mainly on the recording of track sizes suggested the presence of only several animals. Five unique genotypes were identified from the six polymorphic microsatellite loci The genotypic data for each sample were compared with the corresponding track size in order to obtain a minimum estimate of the population size. Because one genotype was found with two nonoverlapping track sizes, we were able to detect six individual bears. The g genotype of one bear was not observed after 1993. The population is composed of one yearling, three adult males and one adult female, This work extends ... |
author2 |
Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Bouvet, J. Taberlet, P. |
author_facet |
Bouvet, J. Taberlet, P. |
author_sort |
Bouvet, J. |
title |
Molecular genetics and field observations to assist Brown Bear population in the Pyrenees |
title_short |
Molecular genetics and field observations to assist Brown Bear population in the Pyrenees |
title_full |
Molecular genetics and field observations to assist Brown Bear population in the Pyrenees |
title_fullStr |
Molecular genetics and field observations to assist Brown Bear population in the Pyrenees |
title_full_unstemmed |
Molecular genetics and field observations to assist Brown Bear population in the Pyrenees |
title_sort |
molecular genetics and field observations to assist brown bear population in the pyrenees |
publisher |
HAL CCSD |
publishDate |
2001 |
url |
https://hal.science/halsde-00280561 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
ISSN: 0037-962X Bulletin De La Societe Zoologique De France https://hal.science/halsde-00280561 Bulletin De La Societe Zoologique De France, 2001, 126 (1-2), pp.179-188 |
op_relation |
halsde-00280561 https://hal.science/halsde-00280561 |
_version_ |
1766231624625684480 |