Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France)
International audience Estuarine zones are extremely fragile due to increasing stress from anthropogenic activities. Amongthose, the Loire estuary (France) is potentially exposed to various contaminants including EndocrineDisruptors Compounds (EDCs) able to impact the reproduction physiology of fish...
Published in: | Ecotoxicology and Environmental Safety |
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Online Access: | https://hal.science/hal-04654563 https://doi.org/10.1016/j.ecoenv.2013.07.019 |
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ftunivnantes:oai:HAL:hal-04654563v1 2024-09-15T17:39:36+00:00 Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) Blanchet-Letrouvé, Isabelle Lafont, Anne-Gaëlle Poirier, Laurence Baloche, Sylvie Zalouk-Vergnoux, Aurore Dufour, Sylvie Mouneyrac, Catherine Mer, molécules et santé EA 2160 (MMS) Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST) Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques Université de Nantes (UN)-Université de Nantes (UN) Mer, molécules et santé (MMS UCO) Université Catholique de l'Ouest (UCO)-PRES Université Nantes Angers Le Mans (UNAM) 2013-11 https://hal.science/hal-04654563 https://doi.org/10.1016/j.ecoenv.2013.07.019 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2013.07.019 hal-04654563 https://hal.science/hal-04654563 doi:10.1016/j.ecoenv.2013.07.019 ISSN: 0147-6513 EISSN: 1090-2414 Ecotoxicology and Environmental Safety https://hal.science/hal-04654563 Ecotoxicology and Environmental Safety, 2013, 97, pp.103-113. ⟨10.1016/j.ecoenv.2013.07.019⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2013 ftunivnantes https://doi.org/10.1016/j.ecoenv.2013.07.019 2024-07-25T23:38:49Z International audience Estuarine zones are extremely fragile due to increasing stress from anthropogenic activities. Amongthose, the Loire estuary (France) is potentially exposed to various contaminants including EndocrineDisruptors Compounds (EDCs) able to impact the reproduction physiology of fish. The European eel(Anguilla anguilla), endangered fish species, is apparently not relevant, in its yellow stage, to monitor theeffects of endocrine disruption. Despite this weakly responsiveness, this study aimed to investigatewhether European eel from the Loire estuary may still be the subject of estrogenic disruption quantifyingthe hepatic Vg gene expression according to gender and sexual stage. Vitellogenin (Vg) appears as avaluable biomarker of EDCs, as well as for exposure and effects. Quantitative real-time ReverseTranscription Polymerase Chain Reaction (q RT PCR) was used in this study to amplify responses ofhepatic Vg transcripts. European eels were sampled in May 2009 (N¼57) and November 2010 (duringthe downstream migration, N¼10) in two sites of the Loire estuary with different ecological conditionsand contamination pressures (upstream: Varades; downstream: Nantes). Reproductive (gender, sexualmaturity stage) and biometric parameters of collected eels were determined. A laboratory exposure ofsilver male to steroid hormones (Testosterone (T), 11-KetoTestosterone (11-KT), Estradiol (E2)) wasconducted in parallel to validate the q RT PCR approach on hepatic Vg mRNA. Results demonstrated theresponsiveness of exposed silver male eels, since hepatic mRNA Vg induction was observed in E2 treatedmales compared to control specimens. In the field, results of female silver eels reflected large interindividual differences in the activation of hepatic Vg at silvering. However, while only female silver eelsshould express hepatic Vg mRNA, quantifiable levels were also detected in a proportion of 38% of theother individuals sampled, normally not inclined to express it, those being undifferentiated eels, yellowfemales, ... Article in Journal/Newspaper Anguilla anguilla European eel Université de Nantes: HAL-UNIV-NANTES Ecotoxicology and Environmental Safety 97 103 113 |
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Université de Nantes: HAL-UNIV-NANTES |
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ftunivnantes |
language |
English |
topic |
[SDE]Environmental Sciences |
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[SDE]Environmental Sciences Blanchet-Letrouvé, Isabelle Lafont, Anne-Gaëlle Poirier, Laurence Baloche, Sylvie Zalouk-Vergnoux, Aurore Dufour, Sylvie Mouneyrac, Catherine Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Estuarine zones are extremely fragile due to increasing stress from anthropogenic activities. Amongthose, the Loire estuary (France) is potentially exposed to various contaminants including EndocrineDisruptors Compounds (EDCs) able to impact the reproduction physiology of fish. The European eel(Anguilla anguilla), endangered fish species, is apparently not relevant, in its yellow stage, to monitor theeffects of endocrine disruption. Despite this weakly responsiveness, this study aimed to investigatewhether European eel from the Loire estuary may still be the subject of estrogenic disruption quantifyingthe hepatic Vg gene expression according to gender and sexual stage. Vitellogenin (Vg) appears as avaluable biomarker of EDCs, as well as for exposure and effects. Quantitative real-time ReverseTranscription Polymerase Chain Reaction (q RT PCR) was used in this study to amplify responses ofhepatic Vg transcripts. European eels were sampled in May 2009 (N¼57) and November 2010 (duringthe downstream migration, N¼10) in two sites of the Loire estuary with different ecological conditionsand contamination pressures (upstream: Varades; downstream: Nantes). Reproductive (gender, sexualmaturity stage) and biometric parameters of collected eels were determined. A laboratory exposure ofsilver male to steroid hormones (Testosterone (T), 11-KetoTestosterone (11-KT), Estradiol (E2)) wasconducted in parallel to validate the q RT PCR approach on hepatic Vg mRNA. Results demonstrated theresponsiveness of exposed silver male eels, since hepatic mRNA Vg induction was observed in E2 treatedmales compared to control specimens. In the field, results of female silver eels reflected large interindividual differences in the activation of hepatic Vg at silvering. However, while only female silver eelsshould express hepatic Vg mRNA, quantifiable levels were also detected in a proportion of 38% of theother individuals sampled, normally not inclined to express it, those being undifferentiated eels, yellowfemales, ... |
author2 |
Mer, molécules et santé EA 2160 (MMS) Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST) Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques Université de Nantes (UN)-Université de Nantes (UN) Mer, molécules et santé (MMS UCO) Université Catholique de l'Ouest (UCO)-PRES Université Nantes Angers Le Mans (UNAM) |
format |
Article in Journal/Newspaper |
author |
Blanchet-Letrouvé, Isabelle Lafont, Anne-Gaëlle Poirier, Laurence Baloche, Sylvie Zalouk-Vergnoux, Aurore Dufour, Sylvie Mouneyrac, Catherine |
author_facet |
Blanchet-Letrouvé, Isabelle Lafont, Anne-Gaëlle Poirier, Laurence Baloche, Sylvie Zalouk-Vergnoux, Aurore Dufour, Sylvie Mouneyrac, Catherine |
author_sort |
Blanchet-Letrouvé, Isabelle |
title |
Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) |
title_short |
Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) |
title_full |
Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) |
title_fullStr |
Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) |
title_full_unstemmed |
Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France) |
title_sort |
vg mrna induction in an endangered fish species (anguilla anguilla) from the loire estuary (france) |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.science/hal-04654563 https://doi.org/10.1016/j.ecoenv.2013.07.019 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
ISSN: 0147-6513 EISSN: 1090-2414 Ecotoxicology and Environmental Safety https://hal.science/hal-04654563 Ecotoxicology and Environmental Safety, 2013, 97, pp.103-113. ⟨10.1016/j.ecoenv.2013.07.019⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2013.07.019 hal-04654563 https://hal.science/hal-04654563 doi:10.1016/j.ecoenv.2013.07.019 |
op_doi |
https://doi.org/10.1016/j.ecoenv.2013.07.019 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
97 |
container_start_page |
103 |
op_container_end_page |
113 |
_version_ |
1810481186580987904 |