Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas )
International audience Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V . parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V . parahaemolyticus accumulation a...
Published in: | Environmental Microbiology |
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Main Authors: | , , , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2022
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Subjects: | |
Online Access: | https://hal.science/hal-04202208 https://hal.science/hal-04202208/document https://hal.science/hal-04202208/file/Life%20history%20of%20oysters%20influences%20Vibrio%20parahaemolyticus%20accumulation%20in%20Pacific%20oysters%20%28Crassostrea%20gigas%29.%20Hervio%20Heath%202022.pdf https://doi.org/10.1111/1462-2920.15996 |
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ftunivnantes:oai:HAL:hal-04202208v1 |
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openpolar |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
spellingShingle |
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) |
topic_facet |
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
description |
International audience Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V . parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V . parahaemolyticus accumulation and depuration in adult Crassostrea gigas , we developed a GFP‐tagged V . parahaemolyticus strain (IFVp201‐ gfp + ), as well as a rapid and efficient quantification method in C . gigas oysters haemolymph by flow cytometry. Impact of the life history of C . gigas on accumulation and depuration of V . parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V . parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates. |
author2 |
Unité Microbiologie Aliment Santé Environnement (MASAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité en Sciences Biologiques et Biotechnologies de Nantes (US2B) Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) Nantes Université - pôle Santé Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) Unité Expérimentale Mollusques Marins Atlantique (EMMA) Unité Adaptation et Santé des Invertébrés Marins (ASIM) |
format |
Article in Journal/Newspaper |
author |
Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique |
author_facet |
Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique |
author_sort |
Sorée, Marion |
title |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) |
title_short |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) |
title_full |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) |
title_fullStr |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) |
title_full_unstemmed |
Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) |
title_sort |
life history of oysters influences vibrio parahaemolyticus accumulation in pacific oysters ( crassostrea gigas ) |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.science/hal-04202208 https://hal.science/hal-04202208/document https://hal.science/hal-04202208/file/Life%20history%20of%20oysters%20influences%20Vibrio%20parahaemolyticus%20accumulation%20in%20Pacific%20oysters%20%28Crassostrea%20gigas%29.%20Hervio%20Heath%202022.pdf https://doi.org/10.1111/1462-2920.15996 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.science/hal-04202208 Environmental Microbiology, 2022, 24 (9), pp.4401-4410. ⟨10.1111/1462-2920.15996⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.15996 hal-04202208 https://hal.science/hal-04202208 https://hal.science/hal-04202208/document https://hal.science/hal-04202208/file/Life%20history%20of%20oysters%20influences%20Vibrio%20parahaemolyticus%20accumulation%20in%20Pacific%20oysters%20%28Crassostrea%20gigas%29.%20Hervio%20Heath%202022.pdf doi:10.1111/1462-2920.15996 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/1462-2920.15996 |
container_title |
Environmental Microbiology |
container_volume |
24 |
container_issue |
9 |
container_start_page |
4401 |
op_container_end_page |
4410 |
_version_ |
1797581560039342080 |
spelling |
ftunivnantes:oai:HAL:hal-04202208v1 2024-04-28T08:16:25+00:00 Life history of oysters influences Vibrio parahaemolyticus accumulation in Pacific oysters ( Crassostrea gigas ) Sorée, Marion Delavat, François Lambert, Christophe Lozach, Solen Papin, Mathias Petton, Bruno Passerini, Delphine Dégremont, Lionel Hervio Heath, Dominique Unité Microbiologie Aliment Santé Environnement (MASAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Unité en Sciences Biologiques et Biotechnologies de Nantes (US2B) Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) Nantes Université - pôle Santé Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) Unité Expérimentale Mollusques Marins Atlantique (EMMA) Unité Adaptation et Santé des Invertébrés Marins (ASIM) 2022-09 https://hal.science/hal-04202208 https://hal.science/hal-04202208/document https://hal.science/hal-04202208/file/Life%20history%20of%20oysters%20influences%20Vibrio%20parahaemolyticus%20accumulation%20in%20Pacific%20oysters%20%28Crassostrea%20gigas%29.%20Hervio%20Heath%202022.pdf https://doi.org/10.1111/1462-2920.15996 en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.15996 hal-04202208 https://hal.science/hal-04202208 https://hal.science/hal-04202208/document https://hal.science/hal-04202208/file/Life%20history%20of%20oysters%20influences%20Vibrio%20parahaemolyticus%20accumulation%20in%20Pacific%20oysters%20%28Crassostrea%20gigas%29.%20Hervio%20Heath%202022.pdf doi:10.1111/1462-2920.15996 info:eu-repo/semantics/OpenAccess ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.science/hal-04202208 Environmental Microbiology, 2022, 24 (9), pp.4401-4410. ⟨10.1111/1462-2920.15996⟩ [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis info:eu-repo/semantics/article Journal articles 2022 ftunivnantes https://doi.org/10.1111/1462-2920.15996 2024-04-04T17:07:06Z International audience Vibrio parahaemolyticus infection in humans is associated with raw oyster consumption. Evaluation of V . parahaemolyticus presence in oysters is of most interest because of the economic and public health issues that it represents. To explore V . parahaemolyticus accumulation and depuration in adult Crassostrea gigas , we developed a GFP‐tagged V . parahaemolyticus strain (IFVp201‐ gfp + ), as well as a rapid and efficient quantification method in C . gigas oysters haemolymph by flow cytometry. Impact of the life history of C . gigas on accumulation and depuration of V . parahaemolyticus IFVp201 was subsequently investigated. We found that naive oysters, i.e. grown in controlled facilities with UV treated seawater, accumulated significantly more IFVp201 than environmental oysters, i.e. grown in intertidal environment. We hypothesized that environmental oysters could have been immune primed, thus could limit V . parahaemolyticus accumulation. Meanwhile, both naive and environmental oysters had similar depuration rates. Article in Journal/Newspaper Crassostrea gigas Université de Nantes: HAL-UNIV-NANTES Environmental Microbiology 24 9 4401 4410 |