SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean
International audience The ecology of the SAR11 clade, the most abundant bacterial group in the ocean, has been intensively studied in temperate and tropical regions, but its distribution remains largely unexplored in the Southern Ocean. Through amplicon sequencing of the 16S rRNA gene, we assessed...
Published in: | Environmental Microbiology Reports |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal-cnrs.archives-ouvertes.fr/hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509/document https://hal-cnrs.archives-ouvertes.fr/hal-03835509/file/Dinasquet-2022-EMIR.pdf https://doi.org/10.1111/1758-2229.13117 |
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ftunivnantes:oai:HAL:hal-03835509v1 2023-05-15T18:24:30+02:00 SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean Dinasquet, Julie Landa, Marine Obernosterer, Ingrid Laboratoire d'Océanographie Microbienne (LOMIC) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2022 https://hal-cnrs.archives-ouvertes.fr/hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509/document https://hal-cnrs.archives-ouvertes.fr/hal-03835509/file/Dinasquet-2022-EMIR.pdf https://doi.org/10.1111/1758-2229.13117 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.13117 hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509/document https://hal-cnrs.archives-ouvertes.fr/hal-03835509/file/Dinasquet-2022-EMIR.pdf doi:10.1111/1758-2229.13117 info:eu-repo/semantics/OpenAccess ISSN: 1758-2229 Environmental Microbiology Reports https://hal-cnrs.archives-ouvertes.fr/hal-03835509 Environmental Microbiology Reports, In press, ⟨10.1111/1758-2229.13117⟩ [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2022 ftunivnantes https://doi.org/10.1111/1758-2229.13117 2023-02-08T02:14:58Z International audience The ecology of the SAR11 clade, the most abundant bacterial group in the ocean, has been intensively studied in temperate and tropical regions, but its distribution remains largely unexplored in the Southern Ocean. Through amplicon sequencing of the 16S rRNA gene, we assessed the contribution of the SAR11 clade to bacterial community composition in the naturally iron fertilized region off Kerguelen Island. We investigated the upper 300 m at seven sites located in early spring phytoplankton blooms and at one high-nutrient low-chlorophyll site. Despite pronounced vertical patterns of the bacterioplankton assemblages, the SAR11 clade had high relative abundances at all depths and sites, averaging 40% (±15%) of the total community relative abundance. Micro-autoradiography combined with CARD-FISH further revealed that the clade had an overall stable contribution (45%–60% in surface waters) to bacterial biomass production (determined by 3H-leucine incorporation) during different early bloom stages. The spatio-temporal partitioning of some of the SAR11 subclades suggests a niche specificity and periodic selection of different subclades in response to the fluctuating extreme conditions of the Southern Ocean. These observations improve our understanding of the ecology of the SAR11 clade and its implications in biogeochemical cycles in the rapidly changing Southern Ocean Article in Journal/Newspaper Southern Ocean Université de Nantes: HAL-UNIV-NANTES Kerguelen Kerguelen Island ENVELOPE(69.500,69.500,-49.250,-49.250) Southern Ocean Environmental Microbiology Reports 14 6 907 916 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Dinasquet, Julie Landa, Marine Obernosterer, Ingrid SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean |
topic_facet |
[SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience The ecology of the SAR11 clade, the most abundant bacterial group in the ocean, has been intensively studied in temperate and tropical regions, but its distribution remains largely unexplored in the Southern Ocean. Through amplicon sequencing of the 16S rRNA gene, we assessed the contribution of the SAR11 clade to bacterial community composition in the naturally iron fertilized region off Kerguelen Island. We investigated the upper 300 m at seven sites located in early spring phytoplankton blooms and at one high-nutrient low-chlorophyll site. Despite pronounced vertical patterns of the bacterioplankton assemblages, the SAR11 clade had high relative abundances at all depths and sites, averaging 40% (±15%) of the total community relative abundance. Micro-autoradiography combined with CARD-FISH further revealed that the clade had an overall stable contribution (45%–60% in surface waters) to bacterial biomass production (determined by 3H-leucine incorporation) during different early bloom stages. The spatio-temporal partitioning of some of the SAR11 subclades suggests a niche specificity and periodic selection of different subclades in response to the fluctuating extreme conditions of the Southern Ocean. These observations improve our understanding of the ecology of the SAR11 clade and its implications in biogeochemical cycles in the rapidly changing Southern Ocean |
author2 |
Laboratoire d'Océanographie Microbienne (LOMIC) Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Dinasquet, Julie Landa, Marine Obernosterer, Ingrid |
author_facet |
Dinasquet, Julie Landa, Marine Obernosterer, Ingrid |
author_sort |
Dinasquet, Julie |
title |
SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean |
title_short |
SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean |
title_full |
SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean |
title_fullStr |
SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean |
title_full_unstemmed |
SAR11 clade microdiversity and activity during the early spring blooms off Kerguelen Island, Southern Ocean |
title_sort |
sar11 clade microdiversity and activity during the early spring blooms off kerguelen island, southern ocean |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal-cnrs.archives-ouvertes.fr/hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509/document https://hal-cnrs.archives-ouvertes.fr/hal-03835509/file/Dinasquet-2022-EMIR.pdf https://doi.org/10.1111/1758-2229.13117 |
long_lat |
ENVELOPE(69.500,69.500,-49.250,-49.250) |
geographic |
Kerguelen Kerguelen Island Southern Ocean |
geographic_facet |
Kerguelen Kerguelen Island Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
ISSN: 1758-2229 Environmental Microbiology Reports https://hal-cnrs.archives-ouvertes.fr/hal-03835509 Environmental Microbiology Reports, In press, ⟨10.1111/1758-2229.13117⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/1758-2229.13117 hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509 https://hal-cnrs.archives-ouvertes.fr/hal-03835509/document https://hal-cnrs.archives-ouvertes.fr/hal-03835509/file/Dinasquet-2022-EMIR.pdf doi:10.1111/1758-2229.13117 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/1758-2229.13117 |
container_title |
Environmental Microbiology Reports |
container_volume |
14 |
container_issue |
6 |
container_start_page |
907 |
op_container_end_page |
916 |
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1766205107386449920 |