Past and present giant viruses diversity explored through permafrost metagenomics
Giant viruses are abundant in aquatic environments and ecologically important through the metabolic reprogramming of their hosts. Lesser is known about giant viruses from soil although two of them, belonging to different families, were reactivated from 30,000-y-old Siberian permafrost samples, sugge...
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ftunivnantes:oai:HAL:hal-03810478v1 2023-05-15T17:56:42+02:00 Past and present giant viruses diversity explored through permafrost metagenomics Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu Information génomique et structurale (IGS) Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) 2022-10-11 https://hal.archives-ouvertes.fr/hal-03810478 https://hal.archives-ouvertes.fr/hal-03810478/document https://hal.archives-ouvertes.fr/hal-03810478/file/v1_covered.pdf https://doi.org/10.21203/rs.3.rs-1328080/v1 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-1328080/v1 hal-03810478 https://hal.archives-ouvertes.fr/hal-03810478 https://hal.archives-ouvertes.fr/hal-03810478/document https://hal.archives-ouvertes.fr/hal-03810478/file/v1_covered.pdf doi:10.21203/rs.3.rs-1328080/v1 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess https://hal.archives-ouvertes.fr/hal-03810478 2022 [SDV]Life Sciences [q-bio] info:eu-repo/semantics/preprint Preprints, Working Papers, . 2022 ftunivnantes https://doi.org/10.21203/rs.3.rs-1328080/v1 2022-10-18T23:05:36Z Giant viruses are abundant in aquatic environments and ecologically important through the metabolic reprogramming of their hosts. Lesser is known about giant viruses from soil although two of them, belonging to different families, were reactivated from 30,000-y-old Siberian permafrost samples, suggesting an untapped diversity of Nucleocytoviricota in this environment. Through permafrost metagenomics, we reveal a high heterogeneity in the abundance of giant viruses representing up to 12% of the total community in one sample. Pithoviridae and Orpheoviridae-like viruses were the most important contributors, followed by Mimiviridae. A comparison to other terrestrial metagenomes confirmed that the diversity pattern in these samples is quite unique. In contrast, Pandoraviridae sequences remained scarce. Using a stringent methodology, we were able to assemble large genomes, including a complete circular 1.6 Mb Pithoviridae-like from a 42,000-y-old sample. The uncovered Pithoviridae diversity also provided insights into the family evolution. The phylogenetic reconstruction of specific functions not only revealed gene transfers between cells and viruses, but also between viruses from different families. Finally, the extensive annotation of the permafrost viral sequences revealed a patchwork of predicted functions amidst an even larger reservoir of anonymous genes of unknown functions. Report permafrost Université de Nantes: HAL-UNIV-NANTES |
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Open Polar |
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Université de Nantes: HAL-UNIV-NANTES |
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ftunivnantes |
language |
English |
topic |
[SDV]Life Sciences [q-bio] |
spellingShingle |
[SDV]Life Sciences [q-bio] Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu Past and present giant viruses diversity explored through permafrost metagenomics |
topic_facet |
[SDV]Life Sciences [q-bio] |
description |
Giant viruses are abundant in aquatic environments and ecologically important through the metabolic reprogramming of their hosts. Lesser is known about giant viruses from soil although two of them, belonging to different families, were reactivated from 30,000-y-old Siberian permafrost samples, suggesting an untapped diversity of Nucleocytoviricota in this environment. Through permafrost metagenomics, we reveal a high heterogeneity in the abundance of giant viruses representing up to 12% of the total community in one sample. Pithoviridae and Orpheoviridae-like viruses were the most important contributors, followed by Mimiviridae. A comparison to other terrestrial metagenomes confirmed that the diversity pattern in these samples is quite unique. In contrast, Pandoraviridae sequences remained scarce. Using a stringent methodology, we were able to assemble large genomes, including a complete circular 1.6 Mb Pithoviridae-like from a 42,000-y-old sample. The uncovered Pithoviridae diversity also provided insights into the family evolution. The phylogenetic reconstruction of specific functions not only revealed gene transfers between cells and viruses, but also between viruses from different families. Finally, the extensive annotation of the permafrost viral sequences revealed a patchwork of predicted functions amidst an even larger reservoir of anonymous genes of unknown functions. |
author2 |
Information génomique et structurale (IGS) Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Report |
author |
Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu |
author_facet |
Rigou, Sofia Santini, Sébastien Abergel, Chantal Claverie, Jean-Michel Legendre, Matthieu |
author_sort |
Rigou, Sofia |
title |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_short |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_full |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_fullStr |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_full_unstemmed |
Past and present giant viruses diversity explored through permafrost metagenomics |
title_sort |
past and present giant viruses diversity explored through permafrost metagenomics |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.archives-ouvertes.fr/hal-03810478 https://hal.archives-ouvertes.fr/hal-03810478/document https://hal.archives-ouvertes.fr/hal-03810478/file/v1_covered.pdf https://doi.org/10.21203/rs.3.rs-1328080/v1 |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
https://hal.archives-ouvertes.fr/hal-03810478 2022 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.21203/rs.3.rs-1328080/v1 hal-03810478 https://hal.archives-ouvertes.fr/hal-03810478 https://hal.archives-ouvertes.fr/hal-03810478/document https://hal.archives-ouvertes.fr/hal-03810478/file/v1_covered.pdf doi:10.21203/rs.3.rs-1328080/v1 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.21203/rs.3.rs-1328080/v1 |
_version_ |
1766164945167187968 |