Exploring the phycosphere of Emiliania huxleyi : from bloom dynamics to microbiome assembly experiments

• Published in: Molecular Ecology
• Main Authors: Câmara dos Reis, Mariana, Romac, Sarah, Le Gall, Florence, Marie, Dominique, Frada, Miguel, Koplovitz, Gil, Cariou, Thierry, Henry, Nicolas, de Vargas, Colomban, Jeanthon, Christian
• Other Authors: Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2023
• Subjects: [SDE]Environmental Sciences; [SDV]Life Sciences [q-bio]; North Atlantic
• Online Access: https://hal.science/hal-03596404; https://hal.science/hal-03596404v2/document; https://hal.science/hal-03596404v2/file/Manuscript_MEC-22-0180_revised_version.pdf; https://doi.org/10.1111/mec.16829

Coccolithophores have global ecological and biogeochemical significance as the most important calcifying marine phytoplankton group. The structure and selection of prokaryotic communities associated with the most abundant coccolithophore and bloom-forming species, Emiliania huxleyi, are still poorly known. In this study, we assessed the diversity of bacterial communities associated with an E. huxleyi bloom in the Celtic Sea (Eastern North Atlantic), exposed axenic E. huxleyi cultures to prokaryotic communities derived from bloom and non-bloom conditions and followed the dynamics of their microbiome composition over one year. Bloom-associated prokaryotic communities were dominated by SAR11, Marine group II Euryarchaeota, Rhodobacterales and contained substantial proportions of known indicators of phytoplankton bloom demises such as Flavobacteriaceae and Pseudoalteromonadaceae. Taxonomic richness of bacteria derived from natural communities that associated with axenic E. huxleyi rapidly shifted and then stabilized over time. The succession of microorganisms recruited from the environment were consistently dependent on the composition of the initial bacterioplankton community. Phycosphere-associated communities derived from the E. huxleyi bloom were highly similar to one another, suggesting deterministic processes, whereas cultures from non-bloom conditions show an effect of stochasticity. Overall, this work sheds new light on the importance of the initial inoculum composition in microbiome recruitment and elucidates the temporal dynamics of its composition and long-term stability.