Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci
International audience The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and co...
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Online Access: | https://hal.inrae.fr/hal-03516844 https://doi.org/10.1007/s10709-019-00053-7 |
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ftunivnantes:oai:HAL:hal-03516844v1 2023-05-15T15:55:33+02:00 Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) LTSER Zone Atelier Armorique (LTSER) Université d'Angers (UA) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH) 2019-02 https://hal.inrae.fr/hal-03516844 https://doi.org/10.1007/s10709-019-00053-7 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10709-019-00053-7 hal-03516844 https://hal.inrae.fr/hal-03516844 doi:10.1007/s10709-019-00053-7 ISSN: 0016-6707 EISSN: 1573-6857 Genetica https://hal.inrae.fr/hal-03516844 Genetica, Springer Verlag, 2019, 147 (1), pp.69-78. ⟨10.1007/s10709-019-00053-7⟩ Raptor Spatial genetic structure Relatedness Microsatellites Circus pygargus Circus cyaneus Circus aeruginosus [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] info:eu-repo/semantics/article Journal articles 2019 ftunivnantes https://doi.org/10.1007/s10709-019-00053-7 2022-08-10T00:46:29Z International audience The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and contemporary gene flow, we developed 16 new microsatellite markers using 454 pyrosequencing. Genotypes of 117 chicks sampled in a 200 × 300 km farmland area in Central Western France were analyzed to characterize genetic polymorphism at each locus and regional and fine-scale genetic structure. Fourteen markers were found polymorphic, with a number of alleles ranging from 3 to 11. The expected and observed heterozygosities ranged from 0.36 to 0.856 and from 0.35 to 0.868, respectively. A single genetic unit was found at the regional scale with higher genetic similarity observed at a small spatial scale (up to 10 km). Our results are consistent with overall large-scale juvenile and adult dispersal together with small-scale male philopatry. Cross-species amplification of this set of microsatellites makers has been successful in two closely related harrier species: the marsh harrier (Circus aeruginosus) and the Hen harrier (Circus cyaneus) for which 14 and 12 markers were polymorphic, respectively. These new microsatellite markers could be used to study the population genetic structure, contemporary gene flow and parentage analyses in these three species and to conduct microsatellite-based demographic inferences on the Montagu’s harrier. Article in Journal/Newspaper Circus cyaneus Université de Nantes: HAL-UNIV-NANTES Hen ENVELOPE(-64.914,-64.914,61.317,61.317) Genetica 147 1 69 78 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Raptor Spatial genetic structure Relatedness Microsatellites Circus pygargus Circus cyaneus Circus aeruginosus [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] |
spellingShingle |
Raptor Spatial genetic structure Relatedness Microsatellites Circus pygargus Circus cyaneus Circus aeruginosus [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
topic_facet |
Raptor Spatial genetic structure Relatedness Microsatellites Circus pygargus Circus cyaneus Circus aeruginosus [SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] |
description |
International audience The Montagu’s harrier (Circus pygargus) is a semi-colonial raptor species widely but patchily distributed across the Palearctic region with recorded cases of philopatry and presence of extra-pair copulation. In order to assess Montagu’s harrier spatial genetic structure and contemporary gene flow, we developed 16 new microsatellite markers using 454 pyrosequencing. Genotypes of 117 chicks sampled in a 200 × 300 km farmland area in Central Western France were analyzed to characterize genetic polymorphism at each locus and regional and fine-scale genetic structure. Fourteen markers were found polymorphic, with a number of alleles ranging from 3 to 11. The expected and observed heterozygosities ranged from 0.36 to 0.856 and from 0.35 to 0.868, respectively. A single genetic unit was found at the regional scale with higher genetic similarity observed at a small spatial scale (up to 10 km). Our results are consistent with overall large-scale juvenile and adult dispersal together with small-scale male philopatry. Cross-species amplification of this set of microsatellites makers has been successful in two closely related harrier species: the marsh harrier (Circus aeruginosus) and the Hen harrier (Circus cyaneus) for which 14 and 12 markers were polymorphic, respectively. These new microsatellite markers could be used to study the population genetic structure, contemporary gene flow and parentage analyses in these three species and to conduct microsatellite-based demographic inferences on the Montagu’s harrier. |
author2 |
Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) LTSER Zone Atelier Armorique (LTSER) Université d'Angers (UA) Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH) |
format |
Article in Journal/Newspaper |
author |
Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand |
author_facet |
Ribout, C. Villers, A. Ruault, S. Bretagnolle, V. Picard, D. Monceau, K. Gauffre, Bertrand |
author_sort |
Ribout, C. |
title |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_short |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_full |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_fullStr |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_full_unstemmed |
Fine-scale genetic structure in a high dispersal capacity raptor, the Montagu’s harrier (Circus pygargus), revealed by a set of novel microsatellite loci |
title_sort |
fine-scale genetic structure in a high dispersal capacity raptor, the montagu’s harrier (circus pygargus), revealed by a set of novel microsatellite loci |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.inrae.fr/hal-03516844 https://doi.org/10.1007/s10709-019-00053-7 |
long_lat |
ENVELOPE(-64.914,-64.914,61.317,61.317) |
geographic |
Hen |
geographic_facet |
Hen |
genre |
Circus cyaneus |
genre_facet |
Circus cyaneus |
op_source |
ISSN: 0016-6707 EISSN: 1573-6857 Genetica https://hal.inrae.fr/hal-03516844 Genetica, Springer Verlag, 2019, 147 (1), pp.69-78. ⟨10.1007/s10709-019-00053-7⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s10709-019-00053-7 hal-03516844 https://hal.inrae.fr/hal-03516844 doi:10.1007/s10709-019-00053-7 |
op_doi |
https://doi.org/10.1007/s10709-019-00053-7 |
container_title |
Genetica |
container_volume |
147 |
container_issue |
1 |
container_start_page |
69 |
op_container_end_page |
78 |
_version_ |
1766391041426980864 |