Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker
International audience The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic da...
Published in: | Infection, Genetics and Evolution |
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Online Access: | https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 https://doi.org/10.1016/j.meegid.2021.104863 |
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ftunivnantes:oai:HAL:hal-03516264v1 2023-05-15T15:15:33+02:00 Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker Umhang, Gérald Bastid, Vanessa Avcioglu, Hamza Bagrade, Guna Bujanić, Miljenko Bjelić Čabrilo, Oliveira Casulli, Adriano Dorny, Pierre van Der Giessen, Joke Guven, Esin Harna, Jiri Karamon, Jacek Kharchenko, Vitaliy Knapp, Jenny Kolarova, Libuse Konyaev, Sergey Laurimaa, Leidi Losch, Serge Miljević, Milan Miterpakova, Martina Moks, Epp Romig, Thomas Saarma, Urmas Snabel, Viliam Sreter, Tamas Valdmann, Harri Boué, Franck Laboratoire Chrono-environnement (UMR 6249) (LCE) Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC) 2021-08 https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 https://doi.org/10.1016/j.meegid.2021.104863 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2021.104863 info:eu-repo/semantics/altIdentifier/pmid/33857665 hal-03516264 https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 doi:10.1016/j.meegid.2021.104863 PUBMED: 33857665 ISSN: 1567-1348 EISSN: 1567-7257 Infection, Genetics and Evolution https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 Infection, Genetics and Evolution, 2021, 92, pp.104863. ⟨10.1016/j.meegid.2021.104863⟩ Asia Echinococcus multilocularis EmsB microsatellite Europe Parasite expansion [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health info:eu-repo/semantics/article Journal articles 2021 ftunivnantes https://doi.org/10.1016/j.meegid.2021.104863 2023-01-18T00:19:36Z International audience The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic data from most eastern European countries is a major knowledge gap, affecting the study of associations with parasite populations in Western Europe. In this study, EmsB microsatellite genotyping of E. multilocularis was performed to describe the genetic diversity and relatedness of 785 E. multilocularis isolates from four western and nine eastern European countries, as well as from Armenia and the Asian parts of Russia and Turkey. The presence of the same E. multilocularis populations in the Benelux resulting from expansion from the historical Alpine focus can be deduced from the main profiles shared between these countries. All 33 EmsB profiles obtained from 528 samples from the nine eastern European countries belonged to the European clade, except one Asian profile form Ryazan Oblast, Russia. The expansion of E. multilocularis seems to have progressed from the historical Alpine focus through Hungary, Slovakia, the Czech Republic and southern Poland towards Latvia and Estonia. Most of the samples from Asia belong to the Asian clade, with one EmsB profile shared between Armenia and Turkey, and two between Turkey and Russia. However, two European profiles were described from two foxes in Turkey, including one harboring worms from both European and Asian clades. Three EmsB profiles from three Russian samples were associated with the Arctic clade. Two E. multilocularis profiles from rodents from Lake Baikal belonged to the Mongolian clade, described for the first time here using EmsB. Further worldwide studies on the genetic diversity of E. multilocularis using both mitochondrial sequencing and EmsB genotyping are needed to understand the distribution and expansion of the various clades. Article in Journal/Newspaper Arctic Université de Nantes: HAL-UNIV-NANTES Arctic Infection, Genetics and Evolution 92 104863 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Asia Echinococcus multilocularis EmsB microsatellite Europe Parasite expansion [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health |
spellingShingle |
Asia Echinococcus multilocularis EmsB microsatellite Europe Parasite expansion [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health Umhang, Gérald Bastid, Vanessa Avcioglu, Hamza Bagrade, Guna Bujanić, Miljenko Bjelić Čabrilo, Oliveira Casulli, Adriano Dorny, Pierre van Der Giessen, Joke Guven, Esin Harna, Jiri Karamon, Jacek Kharchenko, Vitaliy Knapp, Jenny Kolarova, Libuse Konyaev, Sergey Laurimaa, Leidi Losch, Serge Miljević, Milan Miterpakova, Martina Moks, Epp Romig, Thomas Saarma, Urmas Snabel, Viliam Sreter, Tamas Valdmann, Harri Boué, Franck Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker |
topic_facet |
Asia Echinococcus multilocularis EmsB microsatellite Europe Parasite expansion [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health |
description |
International audience The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a severe helminthic zoonotic disease distributed in the Northern Hemisphere. The lifecycle of the parasite is mainly sylvatic, involving canid and rodent hosts. The absence of genetic data from most eastern European countries is a major knowledge gap, affecting the study of associations with parasite populations in Western Europe. In this study, EmsB microsatellite genotyping of E. multilocularis was performed to describe the genetic diversity and relatedness of 785 E. multilocularis isolates from four western and nine eastern European countries, as well as from Armenia and the Asian parts of Russia and Turkey. The presence of the same E. multilocularis populations in the Benelux resulting from expansion from the historical Alpine focus can be deduced from the main profiles shared between these countries. All 33 EmsB profiles obtained from 528 samples from the nine eastern European countries belonged to the European clade, except one Asian profile form Ryazan Oblast, Russia. The expansion of E. multilocularis seems to have progressed from the historical Alpine focus through Hungary, Slovakia, the Czech Republic and southern Poland towards Latvia and Estonia. Most of the samples from Asia belong to the Asian clade, with one EmsB profile shared between Armenia and Turkey, and two between Turkey and Russia. However, two European profiles were described from two foxes in Turkey, including one harboring worms from both European and Asian clades. Three EmsB profiles from three Russian samples were associated with the Arctic clade. Two E. multilocularis profiles from rodents from Lake Baikal belonged to the Mongolian clade, described for the first time here using EmsB. Further worldwide studies on the genetic diversity of E. multilocularis using both mitochondrial sequencing and EmsB genotyping are needed to understand the distribution and expansion of the various clades. |
author2 |
Laboratoire Chrono-environnement (UMR 6249) (LCE) Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC) |
format |
Article in Journal/Newspaper |
author |
Umhang, Gérald Bastid, Vanessa Avcioglu, Hamza Bagrade, Guna Bujanić, Miljenko Bjelić Čabrilo, Oliveira Casulli, Adriano Dorny, Pierre van Der Giessen, Joke Guven, Esin Harna, Jiri Karamon, Jacek Kharchenko, Vitaliy Knapp, Jenny Kolarova, Libuse Konyaev, Sergey Laurimaa, Leidi Losch, Serge Miljević, Milan Miterpakova, Martina Moks, Epp Romig, Thomas Saarma, Urmas Snabel, Viliam Sreter, Tamas Valdmann, Harri Boué, Franck |
author_facet |
Umhang, Gérald Bastid, Vanessa Avcioglu, Hamza Bagrade, Guna Bujanić, Miljenko Bjelić Čabrilo, Oliveira Casulli, Adriano Dorny, Pierre van Der Giessen, Joke Guven, Esin Harna, Jiri Karamon, Jacek Kharchenko, Vitaliy Knapp, Jenny Kolarova, Libuse Konyaev, Sergey Laurimaa, Leidi Losch, Serge Miljević, Milan Miterpakova, Martina Moks, Epp Romig, Thomas Saarma, Urmas Snabel, Viliam Sreter, Tamas Valdmann, Harri Boué, Franck |
author_sort |
Umhang, Gérald |
title |
Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker |
title_short |
Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker |
title_full |
Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker |
title_fullStr |
Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker |
title_full_unstemmed |
Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker |
title_sort |
unravelling the genetic diversity and relatedness of echinococcus multilocularis isolates in eurasia using the emsb microsatellite nuclear marker |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 https://doi.org/10.1016/j.meegid.2021.104863 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1567-1348 EISSN: 1567-7257 Infection, Genetics and Evolution https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 Infection, Genetics and Evolution, 2021, 92, pp.104863. ⟨10.1016/j.meegid.2021.104863⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2021.104863 info:eu-repo/semantics/altIdentifier/pmid/33857665 hal-03516264 https://hal-univ-fcomte.archives-ouvertes.fr/hal-03516264 doi:10.1016/j.meegid.2021.104863 PUBMED: 33857665 |
op_doi |
https://doi.org/10.1016/j.meegid.2021.104863 |
container_title |
Infection, Genetics and Evolution |
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92 |
container_start_page |
104863 |
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1766345920027295744 |