Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas
International audience The ostreid herpesvirus 1 (OsHV-1) and variants were implicated in mass mortality affecting the young Pacific cupped oysters, Crassostrea gigas, in European countries and those around the world. From 2008 onwards, oyster mortality had greatly increased on the French coast and...
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ftunivnantes:oai:HAL:hal-03402140v1 2023-05-15T15:58:04+02:00 Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas Martenot, Claire Lethuillier, Ophélie Fourour, Sarah Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Interactions Cellules Organismes Environnement (ICORE) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2015-11 https://hal-normandie-univ.archives-ouvertes.fr/hal-03402140 https://doi.org/10.1016/j.jip.2015.10.005 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.10.005 hal-03402140 https://hal-normandie-univ.archives-ouvertes.fr/hal-03402140 doi:10.1016/j.jip.2015.10.005 ISSN: 0022-2011 EISSN: 0022-2011 Journal of Invertebrate Pathology https://hal-normandie-univ.archives-ouvertes.fr/hal-03402140 Journal of Invertebrate Pathology, 2015, 132, pp.182-189. ⟨10.1016/j.jip.2015.10.005⟩ Crassostrea gigas Genetic diversity OsHV-1 Variants [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2015 ftunivnantes https://doi.org/10.1016/j.jip.2015.10.005 2022-07-12T23:15:46Z International audience The ostreid herpesvirus 1 (OsHV-1) and variants were implicated in mass mortality affecting the young Pacific cupped oysters, Crassostrea gigas, in European countries and those around the world. From 2008 onwards, oyster mortality had greatly increased on the French coast and was associated with the detection of a new OsHV-1 variant, entitled OsHV-1 μVar. The OsHV-1 μVar is predominant in oysters; however, other OsHV-1 variants have been detected in samples collected during mortality periods or collected out of mortality periods in France, Ireland, Spain, Portugal, Italy, Mexico, United States, South Korea, Australia, and New Zealand. A retrospective study conducted on 1047 OsHV-1 specimens sampled mainly in France between 2009 and 2012, revealed 17 undescribed OsHV-1 variants found in 65 oyster samples. These specimens presented point mutations situated downstream and upstream from the microsatellite area in the C region (ORF 4/5) which were different from the OsHV-1 reference and the OsHV-1 μVar. In the present work, investigation was performed to further characterize these OsHV-1 specimens by sequencing two habitually targeted regions to study genetic polymorphism of the virus: ORF 41/42 and ORF 35-38. An OsHV-1 variant detected in six oyster samples, contained a nucleotide substitution in the C region which impacted the amino acid sequence and might modify the function of the unknown protein encoding by ORF 4. For the ORF 41/42 region, only two specimens presented a synonymous mutation in comparison with the OsHV-1 μVar. All specimens contained the same deletion with the OsHV-1 μVar in ORF 35-38. Then, a phylogenetic analysis based on the C region was performed to investigate the distribution of undescribed specimens among 21 OsHV-1 DNA sequences notified in GenBank and collected from different countries (France, Japan, New Zealand, China, Ireland, and United States) between 1995 and 2012. All analyzed samples and the OsHV-1 μVar were placed in the same group, excepted for a Japan ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES New Zealand Pacific Journal of Invertebrate Pathology 132 182 189 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Crassostrea gigas Genetic diversity OsHV-1 Variants [SDV]Life Sciences [q-bio] |
spellingShingle |
Crassostrea gigas Genetic diversity OsHV-1 Variants [SDV]Life Sciences [q-bio] Martenot, Claire Lethuillier, Ophélie Fourour, Sarah Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas |
topic_facet |
Crassostrea gigas Genetic diversity OsHV-1 Variants [SDV]Life Sciences [q-bio] |
description |
International audience The ostreid herpesvirus 1 (OsHV-1) and variants were implicated in mass mortality affecting the young Pacific cupped oysters, Crassostrea gigas, in European countries and those around the world. From 2008 onwards, oyster mortality had greatly increased on the French coast and was associated with the detection of a new OsHV-1 variant, entitled OsHV-1 μVar. The OsHV-1 μVar is predominant in oysters; however, other OsHV-1 variants have been detected in samples collected during mortality periods or collected out of mortality periods in France, Ireland, Spain, Portugal, Italy, Mexico, United States, South Korea, Australia, and New Zealand. A retrospective study conducted on 1047 OsHV-1 specimens sampled mainly in France between 2009 and 2012, revealed 17 undescribed OsHV-1 variants found in 65 oyster samples. These specimens presented point mutations situated downstream and upstream from the microsatellite area in the C region (ORF 4/5) which were different from the OsHV-1 reference and the OsHV-1 μVar. In the present work, investigation was performed to further characterize these OsHV-1 specimens by sequencing two habitually targeted regions to study genetic polymorphism of the virus: ORF 41/42 and ORF 35-38. An OsHV-1 variant detected in six oyster samples, contained a nucleotide substitution in the C region which impacted the amino acid sequence and might modify the function of the unknown protein encoding by ORF 4. For the ORF 41/42 region, only two specimens presented a synonymous mutation in comparison with the OsHV-1 μVar. All specimens contained the same deletion with the OsHV-1 μVar in ORF 35-38. Then, a phylogenetic analysis based on the C region was performed to investigate the distribution of undescribed specimens among 21 OsHV-1 DNA sequences notified in GenBank and collected from different countries (France, Japan, New Zealand, China, Ireland, and United States) between 1995 and 2012. All analyzed samples and the OsHV-1 μVar were placed in the same group, excepted for a Japan ... |
author2 |
LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Interactions Cellules Organismes Environnement (ICORE) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-CHU Caen Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Martenot, Claire Lethuillier, Ophélie Fourour, Sarah Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline |
author_facet |
Martenot, Claire Lethuillier, Ophélie Fourour, Sarah Oden, Elise Trancart, Suzanne Travaillé, Emmanuelle Houssin, Maryline |
author_sort |
Martenot, Claire |
title |
Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas |
title_short |
Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas |
title_full |
Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas |
title_fullStr |
Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Detection of undescribed ostreid herpesvirus 1 (OsHV-1) specimens from Pacific oyster, Crassostrea gigas |
title_sort |
detection of undescribed ostreid herpesvirus 1 (oshv-1) specimens from pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-03402140 https://doi.org/10.1016/j.jip.2015.10.005 |
geographic |
New Zealand Pacific |
geographic_facet |
New Zealand Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0022-2011 EISSN: 0022-2011 Journal of Invertebrate Pathology https://hal-normandie-univ.archives-ouvertes.fr/hal-03402140 Journal of Invertebrate Pathology, 2015, 132, pp.182-189. ⟨10.1016/j.jip.2015.10.005⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jip.2015.10.005 hal-03402140 https://hal-normandie-univ.archives-ouvertes.fr/hal-03402140 doi:10.1016/j.jip.2015.10.005 |
op_doi |
https://doi.org/10.1016/j.jip.2015.10.005 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
132 |
container_start_page |
182 |
op_container_end_page |
189 |
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1766393788202221568 |