A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016
International audience The acute course of disease in young oysters infected by OsHV-1 and the rapid tissue degradation often preclude histological examination of specimens collected during outbreaks in field. Herein, live spat originated from two geographical areas were sampled just at the onset of...
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 https://doi.org/10.1111/jfd.12883 |
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ftunivnantes:oai:HAL:hal-03400584v1 2023-05-15T17:54:19+02:00 A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 Burioli, Erika A.V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta (IZSTO) Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2018 https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 https://doi.org/10.1111/jfd.12883 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/jfd.12883 hal-03400584 https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 doi:10.1111/jfd.12883 ISSN: 0140-7775 EISSN: 1365-2761 Journal of Fish Diseases https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 Journal of Fish Diseases, Wiley, 2018, 41 (11), pp.1759 - 1769. ⟨10.1111/jfd.12883⟩ genome evolution Herpesvirales mollusc pathology OsHV-1 phylogenetic analysis [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny info:eu-repo/semantics/article Journal articles 2018 ftunivnantes https://doi.org/10.1111/jfd.12883 2022-07-12T23:15:51Z International audience The acute course of disease in young oysters infected by OsHV-1 and the rapid tissue degradation often preclude histological examination of specimens collected during outbreaks in field. Herein, live spat originated from two geographical areas were sampled just at the onset of a mortality event that occurred in Normandy (France) in June 2016. The lesions, associated with high OsHV-1 DNA quantities, were characterized by severe and diffuse haemocytosis mainly involving blast-like cells, myocyte degeneration and large, irregularly shaped degenerate eosinophilic cells in the connective tissue. The herpesvirus was identified by negative staining TEM and real-time PCR. Sequencing of the C region and ORFs 42/43 confirmed that the variants met the definition of OsHV-1 μVar. We sequenced 30 other ORFs in twenty OsHV-1-positive individuals and compared them to the μVar specimens isolated between 2009 and 2011. The ORFs encoding putative membrane proteins Article in Journal/Newspaper Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Journal of Fish Diseases 41 11 1759 1769 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
genome evolution Herpesvirales mollusc pathology OsHV-1 phylogenetic analysis [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny |
spellingShingle |
genome evolution Herpesvirales mollusc pathology OsHV-1 phylogenetic analysis [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny Burioli, Erika A.V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
topic_facet |
genome evolution Herpesvirales mollusc pathology OsHV-1 phylogenetic analysis [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.SA.ZOO]Life Sciences [q-bio]/Agricultural sciences/Zootechny |
description |
International audience The acute course of disease in young oysters infected by OsHV-1 and the rapid tissue degradation often preclude histological examination of specimens collected during outbreaks in field. Herein, live spat originated from two geographical areas were sampled just at the onset of a mortality event that occurred in Normandy (France) in June 2016. The lesions, associated with high OsHV-1 DNA quantities, were characterized by severe and diffuse haemocytosis mainly involving blast-like cells, myocyte degeneration and large, irregularly shaped degenerate eosinophilic cells in the connective tissue. The herpesvirus was identified by negative staining TEM and real-time PCR. Sequencing of the C region and ORFs 42/43 confirmed that the variants met the definition of OsHV-1 μVar. We sequenced 30 other ORFs in twenty OsHV-1-positive individuals and compared them to the μVar specimens isolated between 2009 and 2011. The ORFs encoding putative membrane proteins |
author2 |
LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO) Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta (IZSTO) Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Burioli, Erika A.V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline |
author_facet |
Burioli, Erika A.V. Varello, Katia Lavazza, Antonio Bozzetta, Elena Prearo, Marino Houssin, Maryline |
author_sort |
Burioli, Erika A.V. |
title |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_short |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_full |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_fullStr |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_full_unstemmed |
A novel divergent group of Ostreid herpesvirus 1 μVar variants associated with a mortality event in Pacific oyster spat in Normandy (France) in 2016 |
title_sort |
novel divergent group of ostreid herpesvirus 1 μvar variants associated with a mortality event in pacific oyster spat in normandy (france) in 2016 |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 https://doi.org/10.1111/jfd.12883 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
ISSN: 0140-7775 EISSN: 1365-2761 Journal of Fish Diseases https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 Journal of Fish Diseases, Wiley, 2018, 41 (11), pp.1759 - 1769. ⟨10.1111/jfd.12883⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/jfd.12883 hal-03400584 https://hal-normandie-univ.archives-ouvertes.fr/hal-03400584 doi:10.1111/jfd.12883 |
op_doi |
https://doi.org/10.1111/jfd.12883 |
container_title |
Journal of Fish Diseases |
container_volume |
41 |
container_issue |
11 |
container_start_page |
1759 |
op_container_end_page |
1769 |
_version_ |
1766162060604866560 |