Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification
International audience Elevated amounts of atmospheric CO2 are causing ocean acidification (OA) that may affect marine organisms including fish species. While several studies carried out in fish revealed that OA induces short term dysfunction in sensory systems including regulation of neurons activi...
Published in: | Marine Environmental Research |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2020
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Subjects: | |
Online Access: | https://hal.archives-ouvertes.fr/hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387/document https://hal.archives-ouvertes.fr/hal-02933387/file/S0141113620302658.pdf https://doi.org/10.1016/j.marenvres.2020.105022 |
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ftunivnantes:oai:HAL:hal-02933387v1 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
ELEVATED CO2 IMMUNE-RESPONSE CARBON-DIOXIDE PACIFIC OYSTER RAINBOW-TROUT MARINE FISH IMPACTS C1Q PROTEIN IDENTIFICATION ACL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
ELEVATED CO2 IMMUNE-RESPONSE CARBON-DIOXIDE PACIFIC OYSTER RAINBOW-TROUT MARINE FISH IMPACTS C1Q PROTEIN IDENTIFICATION ACL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology Mazurais, David Servili, Arianna Noel, Cyril Cormier, Alexandre Collet, Sophie Leseur, Romane Le Roy, Maelenn Vitré, Thomas Madec, Lauriane Zambonino-Infante, Jose-Luis Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification |
topic_facet |
ELEVATED CO2 IMMUNE-RESPONSE CARBON-DIOXIDE PACIFIC OYSTER RAINBOW-TROUT MARINE FISH IMPACTS C1Q PROTEIN IDENTIFICATION ACL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Elevated amounts of atmospheric CO2 are causing ocean acidification (OA) that may affect marine organisms including fish species. While several studies carried out in fish revealed that OA induces short term dysfunction in sensory systems including regulation of neurons activity in olfactory epithelium, information on the effects of OA on other physiological processes and actors is scarcer. In the present study we focused our attention on a European sea bass (Dicentrarchus labrax) sghC1q gene, a member of the C1q-domain-containing (C1qDC) protein family. In vertebrates, C1qDC family includes actors involved in different physiological processes including immune response and synaptic organization. Our microsynteny analysis revealed that this sghC1q gene is the orthologous gene in European sea bass to zebrafish (Danio rerio) cbln11 gene. We cloned the full length cbln11 mRNA and identified the different domains (the signal peptide, the coiled coil region and the globular C1q domain) of the deduced protein sequence. Investigation of mRNA expression by qPCR and in situ hybridization revealed that cbln11 gene is especially expressed in the non-sensory epithelium of the olfactory rosette at larval and adult stages. The expression of cbln11 mRNA was analysed by qPCR in the first generation (FO) of European sea bass broodstock exposed since larval stages to water pH of 8.0 (control) or 7.6 (predicted for year 2100) and in their offspring (F1) maintained in the environmental conditions of their parents. Our results showed that cbln11 mRNA expression level was lower in larvae exposed to OA then up-regulated at adult stage in the olfactory rosette of F0 and that this up-regulation is maintained under OA at larval and juvenile stages in F1. Overall, this work provides evidence of a transgenerational inheritance of OA-induced up-regulation of cbln11 gene expression in European sea bass. Further studies will investigate the potential immune function of cbln11 gene and the consequences of these ... |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) LabexMer (OASYS project) ANR-10LABX-0019 Ministry of Ecological and Solidarity Transition Foundation for Biodiversity Research (Ocean Acidification Program, PACIO project) Deutsche ForschungemeinschaftGerman Research Foundation (DFG) PE 1157/8-1, MA4271/3-1 ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) |
format |
Article in Journal/Newspaper |
author |
Mazurais, David Servili, Arianna Noel, Cyril Cormier, Alexandre Collet, Sophie Leseur, Romane Le Roy, Maelenn Vitré, Thomas Madec, Lauriane Zambonino-Infante, Jose-Luis |
author_facet |
Mazurais, David Servili, Arianna Noel, Cyril Cormier, Alexandre Collet, Sophie Leseur, Romane Le Roy, Maelenn Vitré, Thomas Madec, Lauriane Zambonino-Infante, Jose-Luis |
author_sort |
Mazurais, David |
title |
Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification |
title_short |
Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification |
title_full |
Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification |
title_fullStr |
Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification |
title_full_unstemmed |
Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification |
title_sort |
transgenerational regulation of cbln11 gene expression in the olfactory rosette of the european sea bass (dicentrarchus labrax) exposed to ocean acidification |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.archives-ouvertes.fr/hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387/document https://hal.archives-ouvertes.fr/hal-02933387/file/S0141113620302658.pdf https://doi.org/10.1016/j.marenvres.2020.105022 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification Pacific oyster |
genre_facet |
Ocean acidification Pacific oyster |
op_source |
ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research https://hal.archives-ouvertes.fr/hal-02933387 Marine Environmental Research, Elsevier science, 2020, 159, pp.105022. ⟨10.1016/j.marenvres.2020.105022⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2020.105022 info:eu-repo/semantics/altIdentifier/pmid/32662446 hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387/document https://hal.archives-ouvertes.fr/hal-02933387/file/S0141113620302658.pdf doi:10.1016/j.marenvres.2020.105022 PII: S0141-1136(20)30265-8 PUBMED: 32662446 |
op_rights |
http://creativecommons.org/licenses/by-nc/ info:eu-repo/semantics/OpenAccess |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1016/j.marenvres.2020.105022 |
container_title |
Marine Environmental Research |
container_volume |
159 |
container_start_page |
105022 |
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1766158090641604608 |
spelling |
ftunivnantes:oai:HAL:hal-02933387v1 2023-05-15T17:51:05+02:00 Transgenerational regulation of cbln11 gene expression in the olfactory rosette of the European sea bass (Dicentrarchus labrax) exposed to ocean acidification Mazurais, David Servili, Arianna Noel, Cyril Cormier, Alexandre Collet, Sophie Leseur, Romane Le Roy, Maelenn Vitré, Thomas Madec, Lauriane Zambonino-Infante, Jose-Luis Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) LabexMer (OASYS project) ANR-10LABX-0019 Ministry of Ecological and Solidarity Transition Foundation for Biodiversity Research (Ocean Acidification Program, PACIO project) Deutsche ForschungemeinschaftGerman Research Foundation (DFG) PE 1157/8-1, MA4271/3-1 ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010) 2020-07 https://hal.archives-ouvertes.fr/hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387/document https://hal.archives-ouvertes.fr/hal-02933387/file/S0141113620302658.pdf https://doi.org/10.1016/j.marenvres.2020.105022 en eng HAL CCSD Elsevier science info:eu-repo/semantics/altIdentifier/doi/10.1016/j.marenvres.2020.105022 info:eu-repo/semantics/altIdentifier/pmid/32662446 hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387 https://hal.archives-ouvertes.fr/hal-02933387/document https://hal.archives-ouvertes.fr/hal-02933387/file/S0141113620302658.pdf doi:10.1016/j.marenvres.2020.105022 PII: S0141-1136(20)30265-8 PUBMED: 32662446 http://creativecommons.org/licenses/by-nc/ info:eu-repo/semantics/OpenAccess CC-BY-NC ISSN: 0141-1136 EISSN: 1879-0291 Marine Environmental Research https://hal.archives-ouvertes.fr/hal-02933387 Marine Environmental Research, Elsevier science, 2020, 159, pp.105022. ⟨10.1016/j.marenvres.2020.105022⟩ ELEVATED CO2 IMMUNE-RESPONSE CARBON-DIOXIDE PACIFIC OYSTER RAINBOW-TROUT MARINE FISH IMPACTS C1Q PROTEIN IDENTIFICATION ACL [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2020 ftunivnantes https://doi.org/10.1016/j.marenvres.2020.105022 2022-08-23T22:42:07Z International audience Elevated amounts of atmospheric CO2 are causing ocean acidification (OA) that may affect marine organisms including fish species. While several studies carried out in fish revealed that OA induces short term dysfunction in sensory systems including regulation of neurons activity in olfactory epithelium, information on the effects of OA on other physiological processes and actors is scarcer. In the present study we focused our attention on a European sea bass (Dicentrarchus labrax) sghC1q gene, a member of the C1q-domain-containing (C1qDC) protein family. In vertebrates, C1qDC family includes actors involved in different physiological processes including immune response and synaptic organization. Our microsynteny analysis revealed that this sghC1q gene is the orthologous gene in European sea bass to zebrafish (Danio rerio) cbln11 gene. We cloned the full length cbln11 mRNA and identified the different domains (the signal peptide, the coiled coil region and the globular C1q domain) of the deduced protein sequence. Investigation of mRNA expression by qPCR and in situ hybridization revealed that cbln11 gene is especially expressed in the non-sensory epithelium of the olfactory rosette at larval and adult stages. The expression of cbln11 mRNA was analysed by qPCR in the first generation (FO) of European sea bass broodstock exposed since larval stages to water pH of 8.0 (control) or 7.6 (predicted for year 2100) and in their offspring (F1) maintained in the environmental conditions of their parents. Our results showed that cbln11 mRNA expression level was lower in larvae exposed to OA then up-regulated at adult stage in the olfactory rosette of F0 and that this up-regulation is maintained under OA at larval and juvenile stages in F1. Overall, this work provides evidence of a transgenerational inheritance of OA-induced up-regulation of cbln11 gene expression in European sea bass. Further studies will investigate the potential immune function of cbln11 gene and the consequences of these ... Article in Journal/Newspaper Ocean acidification Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Marine Environmental Research 159 105022 |