An integrated investigation of the effects of ocean acidification on adult abalone (Haliotis tuberculata)

• Published in: ICES Journal of Marine Science
• Main Authors: Avignon, Solène, Auzoux-Bordenave, Stéphanie, Martin, Sophie, Dubois, Philippe, Badou, Aïcha, Coheleach, Manon, Richard, Nicolas, Giglio, Sarah, Malet, Loic, Servili, Arianna, Gaillard, Fanny, Huchette, Sylvain, Roussel, Sabine
• Other Authors: Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Adaptation et diversité en milieu marin (ADMM), Institut national des sciences de l'Univers (INSU - CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Marine (LBM), Université libre de Bruxelles (ULB), Station de Biologie Marine de Concarneau, Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Scea France Haliotis, This work was supported in part by the ATM program “Abalone shell mineralization” of the MNHN funded by theMinistère délégué à l’Enseignement Supérieur et à la Recherche (Paris, France), the program “Acidification des Océans” (ICOBio project) funded by the Fondation pour la Recherche sur la Biodiversité (FRB) and the Ministère de la Transition Ecologique et Solidaire (MTES), and the French LabexMER program (OASYS project). SA was sup‐ported by a post-doctoral fellowship from the MNHN funded by the Ministère de la Transition Ecologique et Solidaire (MTES). SDG is holder of a FRIA PhD fellowship from the National Fund for Scientific Research (NFSR, Belgium) and PhD is a Research Director of the NFSR. The Regional Council of Brittany, the General Council of Finistère, the urban community of Concarneau Cornouaille Agglomération, and the European Regional Development Fund (ERDF) are acknowledged for the funding of the scanning electron microscope (Sigma 300 FE-SEM) at the Concarneau Marine Station., ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: shell microstructure; physiology; ocean acidification; mechanical properties; growth; gene expression; abalone; calcification; acl; [SDV.EE.BIO]Life Sciences [q-bio]/Ecology; environment/Bioclimatology; [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography; [SDE.BE]Environmental Sciences/Biodiversity and Ecology; [SDU.OCEAN]Sciences of the Universe [physics]/Ocean; Atmosphere; Ocean acidification
• Online Access: https://hal.sorbonne-universite.fr/hal-02877225; https://hal.sorbonne-universite.fr/hal-02877225/document; https://hal.sorbonne-universite.fr/hal-02877225/file/Avignon%20et%20al.%20-%202020%20-%20An%20integrated%20investigation%20of%20the%20effects%20of%20ocea.pdf; https://doi.org/10.1093/icesjms/fsz257

International audience Ocean acidification (OA) and its subsequent changes in seawater carbonate chemistry are threatening the survival of calcifying organisms. Due to their use of calcium carbonate to build their shells, marine molluscs being are particularly vulnerable[AQ4]. This study investigated the effect of CO 2-induced OA on adult European abalone (Haliotis tuberculata) using a multi-parameter approach. Biological (survival, growth), physiological (pH T of haemolymph, phagocytosis, metabolism, gene expression), and structural responses (shell strength, nano-indentation measurements, Scanning electron microscopy imaging of microstructure) were evaluated throughout a 5-month exposure to ambient (8.0) and low (7.7) pH conditions. During the first 2 months, the haemolymph pH was reduced, indicating that abalone do not compensate for the pH decrease of their internal fluid. Overall metabolism and immune status were not affected, suggesting that abalone maintain their vital functions when facing OA. However, after 4 months of exposure, adverse effects on shell growth, calcification, microstructure, and resistance were highlighted, whereas the haemolymph pH was compensated. Significant reduction in shell mechanical properties was revealed at pH 7.7, suggesting that OA altered the biomineral architecture leading to a more fragile shell. It is concluded that under lower pH, ab-alone metabolism is maintained at a cost to growth and shell integrity. This may impact both abalone ecology and aquaculture.