Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas
International audience Members of the Transforming Growth factor beta (TGF-beta) superfamily of cell signalling polypeptides are known to play important roles in cell proliferation and differentiation during development and in various physiological processes of most animal clades. Recent findings in...
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ftunivnantes:oai:HAL:hal-02664909v1 2024-06-23T07:52:16+00:00 Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas Le Quéré, Hervé Herpin, Amaury Huvet, Arnaud Lelong, Christophe Favrel, Pascal Université de Nantes (UN) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Department of Physiological Chemistry Julius-Maximilians-Universität Würzburg = University of Würzburg (JMU) 2009 https://hal.inrae.fr/hal-02664909 https://doi.org/10.1016/j.gene.2009.01.010 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2009.01.010 hal-02664909 https://hal.inrae.fr/hal-02664909 doi:10.1016/j.gene.2009.01.010 PRODINRA: 273927 WOS: 000265343000013 ISSN: 0378-1119 EISSN: 1879-0038 Gene https://hal.inrae.fr/hal-02664909 Gene, 2009, 436 (1-2), pp.101-107. ⟨10.1016/j.gene.2009.01.010⟩ http://www.sciencedirect.com/science/journal/03781119 development activin receptor mollusc lophotrochozoa nervous tissues [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2009 ftunivnantes https://doi.org/10.1016/j.gene.2009.01.010 2024-06-11T00:06:51Z International audience Members of the Transforming Growth factor beta (TGF-beta) superfamily of cell signalling polypeptides are known to play important roles in cell proliferation and differentiation during development and in various physiological processes of most animal clades. Recent findings in the mollusc Crassostrea gigas demonstrate the occurrence of a diversity of TGF-beta signalling components including various ligands, three type I receptors but only a single type II receptor. This report describes the characterization of Cg-ActRII, a new type II receptor displaying homology with vertebrate and Drosophila Activin type II receptors. The use of zebrafish embryo as a reporter organism revealed that, in a way similar to its zebrafish counterpart, overexpression of Cg-ActRII or its dominant negative acting truncated form resulted in a dose dependent range of dorsoventral defects coupled with anterior disorders. Expression pattern of Cg-ActRII transcripts examined by real time PCR and in situ PCR in C gigas showed high levels of Cg-ActRII transcripts in early embryonic stages and in the developing larval central nervous system. Except for a high expression in the visceral ganglia, most oyster adult tissues displayed rather low levels of transcripts. Altogether, the data suggest a high degree of conservation at both the structural and functional levels during evolution for this class of receptors. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Gene 436 1-2 101 107 |
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Open Polar |
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Université de Nantes: HAL-UNIV-NANTES |
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ftunivnantes |
language |
English |
topic |
development activin receptor mollusc lophotrochozoa nervous tissues [SDV]Life Sciences [q-bio] |
spellingShingle |
development activin receptor mollusc lophotrochozoa nervous tissues [SDV]Life Sciences [q-bio] Le Quéré, Hervé Herpin, Amaury Huvet, Arnaud Lelong, Christophe Favrel, Pascal Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas |
topic_facet |
development activin receptor mollusc lophotrochozoa nervous tissues [SDV]Life Sciences [q-bio] |
description |
International audience Members of the Transforming Growth factor beta (TGF-beta) superfamily of cell signalling polypeptides are known to play important roles in cell proliferation and differentiation during development and in various physiological processes of most animal clades. Recent findings in the mollusc Crassostrea gigas demonstrate the occurrence of a diversity of TGF-beta signalling components including various ligands, three type I receptors but only a single type II receptor. This report describes the characterization of Cg-ActRII, a new type II receptor displaying homology with vertebrate and Drosophila Activin type II receptors. The use of zebrafish embryo as a reporter organism revealed that, in a way similar to its zebrafish counterpart, overexpression of Cg-ActRII or its dominant negative acting truncated form resulted in a dose dependent range of dorsoventral defects coupled with anterior disorders. Expression pattern of Cg-ActRII transcripts examined by real time PCR and in situ PCR in C gigas showed high levels of Cg-ActRII transcripts in early embryonic stages and in the developing larval central nervous system. Except for a high expression in the visceral ganglia, most oyster adult tissues displayed rather low levels of transcripts. Altogether, the data suggest a high degree of conservation at both the structural and functional levels during evolution for this class of receptors. |
author2 |
Université de Nantes (UN) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Department of Physiological Chemistry Julius-Maximilians-Universität Würzburg = University of Würzburg (JMU) |
format |
Article in Journal/Newspaper |
author |
Le Quéré, Hervé Herpin, Amaury Huvet, Arnaud Lelong, Christophe Favrel, Pascal |
author_facet |
Le Quéré, Hervé Herpin, Amaury Huvet, Arnaud Lelong, Christophe Favrel, Pascal |
author_sort |
Le Quéré, Hervé |
title |
Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas |
title_short |
Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas |
title_full |
Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas |
title_fullStr |
Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas |
title_full_unstemmed |
Structural and functional characterizations of an activin type II receptor orthologue from the pacific oyster Crassostrea gigas |
title_sort |
structural and functional characterizations of an activin type ii receptor orthologue from the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.inrae.fr/hal-02664909 https://doi.org/10.1016/j.gene.2009.01.010 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0378-1119 EISSN: 1879-0038 Gene https://hal.inrae.fr/hal-02664909 Gene, 2009, 436 (1-2), pp.101-107. ⟨10.1016/j.gene.2009.01.010⟩ http://www.sciencedirect.com/science/journal/03781119 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gene.2009.01.010 hal-02664909 https://hal.inrae.fr/hal-02664909 doi:10.1016/j.gene.2009.01.010 PRODINRA: 273927 WOS: 000265343000013 |
op_doi |
https://doi.org/10.1016/j.gene.2009.01.010 |
container_title |
Gene |
container_volume |
436 |
container_issue |
1-2 |
container_start_page |
101 |
op_container_end_page |
107 |
_version_ |
1802643517457039360 |