Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway
International audience The small ruminant lentiviruses, caprine arthritis-encephalitis virus (CAEV) and maedi visna virus (MVV) naturally cause inflammatory disease in goats and sheep, provoking chronic lesions in several different organs. We have previously demonstrated that in vitro infection of c...
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Online Access: | https://hal.inrae.fr/hal-02662460 https://doi.org/10.1016/j.virol.2008.09.039 |
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ftunivnantes:oai:HAL:hal-02662460v1 2023-05-15T16:29:11+02:00 Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway Rea-Boutrois, Angela Villet, Stéphanie Greenland, Timothy Mehlen, Patrick Chebloune, Yahia Verdier, Gérard Legras-Lachuer, Catherine Rétrovirus et Pathologie Comparée (RPC) Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL) École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL) BioSciences Lyon-Gerland (BLG) École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) Physiopathologie moléculaire et nouveaux traitements des hépatites virales Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-IFR62-Institut National de la Santé et de la Recherche Médicale (INSERM) Centre Léon Bérard Lyon University of Kansas Lawrence (KU) 2009 https://hal.inrae.fr/hal-02662460 https://doi.org/10.1016/j.virol.2008.09.039 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2008.09.039 hal-02662460 https://hal.inrae.fr/hal-02662460 doi:10.1016/j.virol.2008.09.039 PRODINRA: 144531 WOS: 000262447300012 ISSN: 0042-6822 EISSN: 1096-0341 Virology https://hal.inrae.fr/hal-02662460 Virology, Elsevier, 2009, 383 (1), pp.93-102. ⟨10.1016/j.virol.2008.09.039⟩ CAEV TAT INTRINSIC PATHWAY MVV APOPTOSIS [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2009 ftunivnantes https://doi.org/10.1016/j.virol.2008.09.039 2022-10-05T00:08:54Z International audience The small ruminant lentiviruses, caprine arthritis-encephalitis virus (CAEV) and maedi visna virus (MVV) naturally cause inflammatory disease in goats and sheep, provoking chronic lesions in several different organs. We have previously demonstrated that in vitro infection of caprine cells by CAEV induces apoptosis through the intrinsic pathway (Rea-Boutrois, A., Pontini, G., Greenland, T., Mehlen, P., Chebloune, Y., Verdier, G. and Legras-Lachuer, C. 2008). In the present Study, we used Tat deleted viruses and SLIZV Tat-expression vectors to show that the SRLV Tat proteins are responsible for this apoptosis. We have also studied the activation of caspases-3, -8 and -9 by fluorescent assays in caprine cells expressing SRLV Tat proteins, and the effects of transfected dominant negative variants of these caspases, to show that Tat-associated apoptosis depends on activation of caspases-3 and -9, but not -8. A simultaneous disruption of mitochondrial membrane potential indicates an involvement of the mitochondrial pathway. Article in Journal/Newspaper Greenland Université de Nantes: HAL-UNIV-NANTES Greenland Verdier ENVELOPE(-60.883,-60.883,-66.033,-66.033) Virology 383 1 93 102 |
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Open Polar |
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Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
CAEV TAT INTRINSIC PATHWAY MVV APOPTOSIS [SDV]Life Sciences [q-bio] |
spellingShingle |
CAEV TAT INTRINSIC PATHWAY MVV APOPTOSIS [SDV]Life Sciences [q-bio] Rea-Boutrois, Angela Villet, Stéphanie Greenland, Timothy Mehlen, Patrick Chebloune, Yahia Verdier, Gérard Legras-Lachuer, Catherine Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
topic_facet |
CAEV TAT INTRINSIC PATHWAY MVV APOPTOSIS [SDV]Life Sciences [q-bio] |
description |
International audience The small ruminant lentiviruses, caprine arthritis-encephalitis virus (CAEV) and maedi visna virus (MVV) naturally cause inflammatory disease in goats and sheep, provoking chronic lesions in several different organs. We have previously demonstrated that in vitro infection of caprine cells by CAEV induces apoptosis through the intrinsic pathway (Rea-Boutrois, A., Pontini, G., Greenland, T., Mehlen, P., Chebloune, Y., Verdier, G. and Legras-Lachuer, C. 2008). In the present Study, we used Tat deleted viruses and SLIZV Tat-expression vectors to show that the SRLV Tat proteins are responsible for this apoptosis. We have also studied the activation of caspases-3, -8 and -9 by fluorescent assays in caprine cells expressing SRLV Tat proteins, and the effects of transfected dominant negative variants of these caspases, to show that Tat-associated apoptosis depends on activation of caspases-3 and -9, but not -8. A simultaneous disruption of mitochondrial membrane potential indicates an involvement of the mitochondrial pathway. |
author2 |
Rétrovirus et Pathologie Comparée (RPC) Institut National de la Recherche Agronomique (INRA)-École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL) École pratique des hautes études (EPHE) Université Paris sciences et lettres (PSL) BioSciences Lyon-Gerland (BLG) École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) Physiopathologie moléculaire et nouveaux traitements des hépatites virales Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-IFR62-Institut National de la Santé et de la Recherche Médicale (INSERM) Centre Léon Bérard Lyon University of Kansas Lawrence (KU) |
format |
Article in Journal/Newspaper |
author |
Rea-Boutrois, Angela Villet, Stéphanie Greenland, Timothy Mehlen, Patrick Chebloune, Yahia Verdier, Gérard Legras-Lachuer, Catherine |
author_facet |
Rea-Boutrois, Angela Villet, Stéphanie Greenland, Timothy Mehlen, Patrick Chebloune, Yahia Verdier, Gérard Legras-Lachuer, Catherine |
author_sort |
Rea-Boutrois, Angela |
title |
Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
title_short |
Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
title_full |
Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
title_fullStr |
Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
title_full_unstemmed |
Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
title_sort |
small ruminant lentivirus tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.inrae.fr/hal-02662460 https://doi.org/10.1016/j.virol.2008.09.039 |
long_lat |
ENVELOPE(-60.883,-60.883,-66.033,-66.033) |
geographic |
Greenland Verdier |
geographic_facet |
Greenland Verdier |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
ISSN: 0042-6822 EISSN: 1096-0341 Virology https://hal.inrae.fr/hal-02662460 Virology, Elsevier, 2009, 383 (1), pp.93-102. ⟨10.1016/j.virol.2008.09.039⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virol.2008.09.039 hal-02662460 https://hal.inrae.fr/hal-02662460 doi:10.1016/j.virol.2008.09.039 PRODINRA: 144531 WOS: 000262447300012 |
op_doi |
https://doi.org/10.1016/j.virol.2008.09.039 |
container_title |
Virology |
container_volume |
383 |
container_issue |
1 |
container_start_page |
93 |
op_container_end_page |
102 |
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1766018873678626816 |