A tolloid homologue from the pacific oyster Crassostrea gigas
International audience The genes governing mesoderm specification have been extensively studied in vertebrates, arthropods and nematodes. The latter two phyla belong to the Ecdysozoan clade but little is understood of the role that these genes might play in the development of the other major protost...
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Online Access: | https://hal.inrae.fr/hal-02657862 https://doi.org/10.1016/j.modgep.2007.03.001 |
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ftunivnantes:oai:HAL:hal-02657862v1 2023-05-15T15:57:40+02:00 A tolloid homologue from the pacific oyster Crassostrea gigas Herpin, Amaury Lelong, Christophe Becker, Tom Favrel, Pascal Cunningham, Charles SARS International Centre for Marine Molecular Biology Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Nantes (UN) Basse-Normandie Regional Council, France and the French-Norwegian Foundation for Scientific, Technical and Industrial Research 2007 https://hal.inrae.fr/hal-02657862 https://doi.org/10.1016/j.modgep.2007.03.001 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.modgep.2007.03.001 info:eu-repo/semantics/altIdentifier/pmid/17433792 hal-02657862 https://hal.inrae.fr/hal-02657862 doi:10.1016/j.modgep.2007.03.001 PRODINRA: 273955 PUBMED: 17433792 WOS: 000247481800007 ISSN: 1567-133X Gene Expression Patterns https://hal.inrae.fr/hal-02657862 Gene Expression Patterns, 2007, 7 (6), pp.700-708. ⟨10.1016/j.modgep.2007.03.001⟩ http://www.journals.elsevier.com/gene-expression-patterns/ Crassostrea gigas bivalve zebrafish Ecdysozoa bone morphogenetic protein transforming growth factor beta mesoderm patterning embryogenesis tolloid oyster Lophotrochozoa mollusc [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2007 ftunivnantes https://doi.org/10.1016/j.modgep.2007.03.001 2023-03-15T15:26:04Z International audience The genes governing mesoderm specification have been extensively studied in vertebrates, arthropods and nematodes. The latter two phyla belong to the Ecdysozoan clade but little is understood of the role that these genes might play in the development of the other major protostomal clade, the Lophotrochozoa. As part of a wider project to analyze the functions associated with transforming growth factor beta superfamily members in Lophotrochozoa, we have cloned a gene encoding a tolloid homologue from the bivalve mollusc Crassostrea gigas. Tolloid is a key developmental protein that regulates the activity of bone morphogenetic proteins (BMPs). We have determined the intron-exon structure of the gene encoding C. gigas tolloid and have compared it with those of homologous genes from both protostomes and deuterostomes. In order to analyze the functionality of oyster tolloid the zebrafish embryo has been employed as a reporter organism and we show that over-expression of this protein results in the ventralization of zebrafish embryos at 24h post fertilization. The expression of the C. gigas tolloid gene during embryonic and larval development as well as in adult tissues is also explored. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Gene Expression Patterns 7 6 700 708 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Crassostrea gigas bivalve zebrafish Ecdysozoa bone morphogenetic protein transforming growth factor beta mesoderm patterning embryogenesis tolloid oyster Lophotrochozoa mollusc [SDV]Life Sciences [q-bio] |
spellingShingle |
Crassostrea gigas bivalve zebrafish Ecdysozoa bone morphogenetic protein transforming growth factor beta mesoderm patterning embryogenesis tolloid oyster Lophotrochozoa mollusc [SDV]Life Sciences [q-bio] Herpin, Amaury Lelong, Christophe Becker, Tom Favrel, Pascal Cunningham, Charles A tolloid homologue from the pacific oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas bivalve zebrafish Ecdysozoa bone morphogenetic protein transforming growth factor beta mesoderm patterning embryogenesis tolloid oyster Lophotrochozoa mollusc [SDV]Life Sciences [q-bio] |
description |
International audience The genes governing mesoderm specification have been extensively studied in vertebrates, arthropods and nematodes. The latter two phyla belong to the Ecdysozoan clade but little is understood of the role that these genes might play in the development of the other major protostomal clade, the Lophotrochozoa. As part of a wider project to analyze the functions associated with transforming growth factor beta superfamily members in Lophotrochozoa, we have cloned a gene encoding a tolloid homologue from the bivalve mollusc Crassostrea gigas. Tolloid is a key developmental protein that regulates the activity of bone morphogenetic proteins (BMPs). We have determined the intron-exon structure of the gene encoding C. gigas tolloid and have compared it with those of homologous genes from both protostomes and deuterostomes. In order to analyze the functionality of oyster tolloid the zebrafish embryo has been employed as a reporter organism and we show that over-expression of this protein results in the ventralization of zebrafish embryos at 24h post fertilization. The expression of the C. gigas tolloid gene during embryonic and larval development as well as in adult tissues is also explored. |
author2 |
SARS International Centre for Marine Molecular Biology Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Université de Nantes (UN) Basse-Normandie Regional Council, France and the French-Norwegian Foundation for Scientific, Technical and Industrial Research |
format |
Article in Journal/Newspaper |
author |
Herpin, Amaury Lelong, Christophe Becker, Tom Favrel, Pascal Cunningham, Charles |
author_facet |
Herpin, Amaury Lelong, Christophe Becker, Tom Favrel, Pascal Cunningham, Charles |
author_sort |
Herpin, Amaury |
title |
A tolloid homologue from the pacific oyster Crassostrea gigas |
title_short |
A tolloid homologue from the pacific oyster Crassostrea gigas |
title_full |
A tolloid homologue from the pacific oyster Crassostrea gigas |
title_fullStr |
A tolloid homologue from the pacific oyster Crassostrea gigas |
title_full_unstemmed |
A tolloid homologue from the pacific oyster Crassostrea gigas |
title_sort |
tolloid homologue from the pacific oyster crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.inrae.fr/hal-02657862 https://doi.org/10.1016/j.modgep.2007.03.001 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1567-133X Gene Expression Patterns https://hal.inrae.fr/hal-02657862 Gene Expression Patterns, 2007, 7 (6), pp.700-708. ⟨10.1016/j.modgep.2007.03.001⟩ http://www.journals.elsevier.com/gene-expression-patterns/ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.modgep.2007.03.001 info:eu-repo/semantics/altIdentifier/pmid/17433792 hal-02657862 https://hal.inrae.fr/hal-02657862 doi:10.1016/j.modgep.2007.03.001 PRODINRA: 273955 PUBMED: 17433792 WOS: 000247481800007 |
op_doi |
https://doi.org/10.1016/j.modgep.2007.03.001 |
container_title |
Gene Expression Patterns |
container_volume |
7 |
container_issue |
6 |
container_start_page |
700 |
op_container_end_page |
708 |
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1766393335385161728 |