Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile.
International audience In this study, we isolated, identified and characterized isolates of Tenacibaculum dicentrarchi in Atlantic salmon (Salmo salar) farmed in Chile for the first time. In 2010 and 2014, mortalities were observed in Atlantic salmon (average weight 25-30 and 480-520 g, respectively...
Published in: | Transboundary and Emerging Diseases |
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Online Access: | https://hal.inrae.fr/hal-02630477 https://doi.org/10.1111/tbed.12464 |
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ftunivnantes:oai:HAL:hal-02630477v1 2023-05-15T15:27:41+02:00 Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. Avendaño-Herrera, R. Irgang, R. Sandoval, C. Moreno-Lira, P. Houel, Armel, Duchaud, Éric Poblete-Morales, M. Nicolas, Pierre, Ilardi, P. Universidad Andrés Bello Santiago (UNAB) Interdisciplinary Center for Aquaculture Research Universidad de Concepción - University of Concepcion Chile Research and Development Laboratory Etablissement Français du Sang Auvergne-Rhône-Alpes Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Mathématiques et Informatique Appliquées du Génome à l'Environnement Jouy-En-Josas (MaIAGE) 2016 https://hal.inrae.fr/hal-02630477 https://doi.org/10.1111/tbed.12464 en eng HAL CCSD Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/tbed.12464 info:eu-repo/semantics/altIdentifier/pmid/26749435 hal-02630477 https://hal.inrae.fr/hal-02630477 doi:10.1111/tbed.12464 PRODINRA: 344506 PUBMED: 26749435 WOS: 000371720900018 ISSN: 1865-1674 EISSN: 1865-1682 Transboundary and emerging diseases https://hal.inrae.fr/hal-02630477 Transboundary and emerging diseases, 2016, 63 (2), pp.121-126. ⟨10.1111/tbed.12464⟩ Tenacibaculum dicentrarchi Atlantic salmon Chile Coho salmon outbreaks rainbow trout [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2016 ftunivnantes https://doi.org/10.1111/tbed.12464 2023-02-01T00:46:19Z International audience In this study, we isolated, identified and characterized isolates of Tenacibaculum dicentrarchi in Atlantic salmon (Salmo salar) farmed in Chile for the first time. In 2010 and 2014, mortalities were observed in Atlantic salmon (average weight 25-30 and 480-520 g, respectively) at an aquaculture centre in Puerto Montt, Chile. Severe tail rots, frayed fins and, in some cases, damaged gills were detected. Wet smear analyses of these lesions revealed a high occurrence of Gram-negative, filamentous bacteria. Microbiological analysis of infected gill and tail tissues yielded six bacterial isolates. All were identified as T. dicentrarchi through polyphasic taxonomy, which included phenotypic characterization, 16S rRNA sequencing and multilocus sequence typing. The latter method revealed a close relationship of the Chilean genotype with the T. dicentrarchi type strain and two Norwegian Atlantic cod (Gadus morhua) isolates. The pathogenic potential of the TdChD05 isolate was assessed by challenging Atlantic salmon and rainbow trout (Oncorhynchus mykiss) for one hour, which resulted in mean cumulative mortality rates of 65% and 93%, respectively, as well as clinical signs 14 days post-challenge. However, challenged Coho salmon (Oncorhynchus kisutch) presented no mortalities or clinical signs of infection. These findings indicate that the geographical and host distribution of T. dicentrarchi is wider than previously established and that this bacterium may have negative impacts on salmonid cultures. Article in Journal/Newspaper atlantic cod Atlantic salmon Gadus morhua Salmo salar Université de Nantes: HAL-UNIV-NANTES Transboundary and Emerging Diseases 63 2 121 126 |
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Open Polar |
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Université de Nantes: HAL-UNIV-NANTES |
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ftunivnantes |
language |
English |
topic |
Tenacibaculum dicentrarchi Atlantic salmon Chile Coho salmon outbreaks rainbow trout [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] |
spellingShingle |
Tenacibaculum dicentrarchi Atlantic salmon Chile Coho salmon outbreaks rainbow trout [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] Avendaño-Herrera, R. Irgang, R. Sandoval, C. Moreno-Lira, P. Houel, Armel, Duchaud, Éric Poblete-Morales, M. Nicolas, Pierre, Ilardi, P. Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. |
topic_facet |
Tenacibaculum dicentrarchi Atlantic salmon Chile Coho salmon outbreaks rainbow trout [MATH]Mathematics [math] [INFO]Computer Science [cs] [SDV]Life Sciences [q-bio] |
description |
International audience In this study, we isolated, identified and characterized isolates of Tenacibaculum dicentrarchi in Atlantic salmon (Salmo salar) farmed in Chile for the first time. In 2010 and 2014, mortalities were observed in Atlantic salmon (average weight 25-30 and 480-520 g, respectively) at an aquaculture centre in Puerto Montt, Chile. Severe tail rots, frayed fins and, in some cases, damaged gills were detected. Wet smear analyses of these lesions revealed a high occurrence of Gram-negative, filamentous bacteria. Microbiological analysis of infected gill and tail tissues yielded six bacterial isolates. All were identified as T. dicentrarchi through polyphasic taxonomy, which included phenotypic characterization, 16S rRNA sequencing and multilocus sequence typing. The latter method revealed a close relationship of the Chilean genotype with the T. dicentrarchi type strain and two Norwegian Atlantic cod (Gadus morhua) isolates. The pathogenic potential of the TdChD05 isolate was assessed by challenging Atlantic salmon and rainbow trout (Oncorhynchus mykiss) for one hour, which resulted in mean cumulative mortality rates of 65% and 93%, respectively, as well as clinical signs 14 days post-challenge. However, challenged Coho salmon (Oncorhynchus kisutch) presented no mortalities or clinical signs of infection. These findings indicate that the geographical and host distribution of T. dicentrarchi is wider than previously established and that this bacterium may have negative impacts on salmonid cultures. |
author2 |
Universidad Andrés Bello Santiago (UNAB) Interdisciplinary Center for Aquaculture Research Universidad de Concepción - University of Concepcion Chile Research and Development Laboratory Etablissement Français du Sang Auvergne-Rhône-Alpes Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Mathématiques et Informatique Appliquées du Génome à l'Environnement Jouy-En-Josas (MaIAGE) |
format |
Article in Journal/Newspaper |
author |
Avendaño-Herrera, R. Irgang, R. Sandoval, C. Moreno-Lira, P. Houel, Armel, Duchaud, Éric Poblete-Morales, M. Nicolas, Pierre, Ilardi, P. |
author_facet |
Avendaño-Herrera, R. Irgang, R. Sandoval, C. Moreno-Lira, P. Houel, Armel, Duchaud, Éric Poblete-Morales, M. Nicolas, Pierre, Ilardi, P. |
author_sort |
Avendaño-Herrera, R. |
title |
Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. |
title_short |
Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. |
title_full |
Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. |
title_fullStr |
Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. |
title_full_unstemmed |
Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile. |
title_sort |
isolation, characterization and virulence potential of tenacibaculum dicentrarchi in salmonid cultures in chile. |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://hal.inrae.fr/hal-02630477 https://doi.org/10.1111/tbed.12464 |
genre |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
op_source |
ISSN: 1865-1674 EISSN: 1865-1682 Transboundary and emerging diseases https://hal.inrae.fr/hal-02630477 Transboundary and emerging diseases, 2016, 63 (2), pp.121-126. ⟨10.1111/tbed.12464⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/tbed.12464 info:eu-repo/semantics/altIdentifier/pmid/26749435 hal-02630477 https://hal.inrae.fr/hal-02630477 doi:10.1111/tbed.12464 PRODINRA: 344506 PUBMED: 26749435 WOS: 000371720900018 |
op_doi |
https://doi.org/10.1111/tbed.12464 |
container_title |
Transboundary and Emerging Diseases |
container_volume |
63 |
container_issue |
2 |
container_start_page |
121 |
op_container_end_page |
126 |
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1766358099863535616 |