Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis
International audience During winter in the Arctic marine ecosystem, diatoms have to survive long periods of darkness caused by low sun elevations and the presence of sea ice covered by snow. To better understand how diatoms survive in the dark, we subjected cultures of the Arctic diatom Chaetoceros...
Published in: | Polar Biology |
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Online Access: | https://hal.archives-ouvertes.fr/hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125/document https://hal.archives-ouvertes.fr/hal-02415125/file/64597.pdf https://doi.org/10.1007/s00300-019-02507-2 |
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ftunivnantes:oai:HAL:hal-02415125v1 2023-05-15T14:52:02+02:00 Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis Lacour, Thomas Morin, Philippe-Israël Sciandra, Théo Donaher, Natalie Campbell, Douglas Ferland, Joannie Babin, Marcel Takuvik Joint International Laboratory ULAVAL-CNRS Université Laval Québec (ULaval)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Mount Allison University 2019-10 https://hal.archives-ouvertes.fr/hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125/document https://hal.archives-ouvertes.fr/hal-02415125/file/64597.pdf https://doi.org/10.1007/s00300-019-02507-2 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-019-02507-2 hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125/document https://hal.archives-ouvertes.fr/hal-02415125/file/64597.pdf doi:10.1007/s00300-019-02507-2 info:eu-repo/semantics/OpenAccess ISSN: 0722-4060 EISSN: 1432-2056 Polar Biology https://hal.archives-ouvertes.fr/hal-02415125 Polar Biology, Springer Verlag, 2019, 42 (10), pp.1787-1799. ⟨10.1007/s00300-019-02507-2⟩ Arctic microalgae Polar night Diatom Darkness Photosynthesis Growth rate Temperature [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography info:eu-repo/semantics/article Journal articles 2019 ftunivnantes https://doi.org/10.1007/s00300-019-02507-2 2022-07-12T22:57:32Z International audience During winter in the Arctic marine ecosystem, diatoms have to survive long periods of darkness caused by low sun elevations and the presence of sea ice covered by snow. To better understand how diatoms survive in the dark, we subjected cultures of the Arctic diatom Chaetoceros neogracilis to a prolonged period of darkness (1 month) and to light resupply. Chaetoceros neogracilis was not able to grow in the dark but cell biovolume remained constant after 1 month in darkness. Rapid resumption of photosynthesis and growth recovery was also found when the cells were transferred back to light at four different light levels ranging from 5 to 154 µmol photon m−2 s−1. This demonstrates the remarkable ability of this species to re-initiate growth over a wide range of irradiances even after a prolonged period in the dark with no apparent lag period or impact on survival. Such recovery was possible because C. neogracilis cells preserved their Chl a content and their light absorption capabilities. Carbon fixation capacity was down-regulated (ninefold dark decrease in PCm) much more than was the photochemistry in PSII (2.3-fold dark decrease in ETRm). Rubisco content, which remained unchanged after one month in the dark, was not responsible for the decrease in PCm. The decrease in PSII activity was partially related to the induction of sustained non-photochemical quenching (NPQ) as we observed an increase in diatoxanthin content after one month in the dark. Article in Journal/Newspaper Arctic Polar Biology polar night Sea ice Université de Nantes: HAL-UNIV-NANTES Arctic Polar Biology 42 10 1787 1799 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Arctic microalgae Polar night Diatom Darkness Photosynthesis Growth rate Temperature [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
spellingShingle |
Arctic microalgae Polar night Diatom Darkness Photosynthesis Growth rate Temperature [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography Lacour, Thomas Morin, Philippe-Israël Sciandra, Théo Donaher, Natalie Campbell, Douglas Ferland, Joannie Babin, Marcel Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis |
topic_facet |
Arctic microalgae Polar night Diatom Darkness Photosynthesis Growth rate Temperature [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography |
description |
International audience During winter in the Arctic marine ecosystem, diatoms have to survive long periods of darkness caused by low sun elevations and the presence of sea ice covered by snow. To better understand how diatoms survive in the dark, we subjected cultures of the Arctic diatom Chaetoceros neogracilis to a prolonged period of darkness (1 month) and to light resupply. Chaetoceros neogracilis was not able to grow in the dark but cell biovolume remained constant after 1 month in darkness. Rapid resumption of photosynthesis and growth recovery was also found when the cells were transferred back to light at four different light levels ranging from 5 to 154 µmol photon m−2 s−1. This demonstrates the remarkable ability of this species to re-initiate growth over a wide range of irradiances even after a prolonged period in the dark with no apparent lag period or impact on survival. Such recovery was possible because C. neogracilis cells preserved their Chl a content and their light absorption capabilities. Carbon fixation capacity was down-regulated (ninefold dark decrease in PCm) much more than was the photochemistry in PSII (2.3-fold dark decrease in ETRm). Rubisco content, which remained unchanged after one month in the dark, was not responsible for the decrease in PCm. The decrease in PSII activity was partially related to the induction of sustained non-photochemical quenching (NPQ) as we observed an increase in diatoxanthin content after one month in the dark. |
author2 |
Takuvik Joint International Laboratory ULAVAL-CNRS Université Laval Québec (ULaval)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Mount Allison University |
format |
Article in Journal/Newspaper |
author |
Lacour, Thomas Morin, Philippe-Israël Sciandra, Théo Donaher, Natalie Campbell, Douglas Ferland, Joannie Babin, Marcel |
author_facet |
Lacour, Thomas Morin, Philippe-Israël Sciandra, Théo Donaher, Natalie Campbell, Douglas Ferland, Joannie Babin, Marcel |
author_sort |
Lacour, Thomas |
title |
Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis |
title_short |
Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis |
title_full |
Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis |
title_fullStr |
Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis |
title_full_unstemmed |
Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis |
title_sort |
decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the arctic diatom chaetoceros neogracilis |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.archives-ouvertes.fr/hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125/document https://hal.archives-ouvertes.fr/hal-02415125/file/64597.pdf https://doi.org/10.1007/s00300-019-02507-2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Polar Biology polar night Sea ice |
genre_facet |
Arctic Polar Biology polar night Sea ice |
op_source |
ISSN: 0722-4060 EISSN: 1432-2056 Polar Biology https://hal.archives-ouvertes.fr/hal-02415125 Polar Biology, Springer Verlag, 2019, 42 (10), pp.1787-1799. ⟨10.1007/s00300-019-02507-2⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-019-02507-2 hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125 https://hal.archives-ouvertes.fr/hal-02415125/document https://hal.archives-ouvertes.fr/hal-02415125/file/64597.pdf doi:10.1007/s00300-019-02507-2 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s00300-019-02507-2 |
container_title |
Polar Biology |
container_volume |
42 |
container_issue |
10 |
container_start_page |
1787 |
op_container_end_page |
1799 |
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1766323155168657408 |