Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge
A bacterial strain, designated BAR1(T), was isolated from a microbial mat growing on the surface of a barite chimney at the Loki's Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1(T) were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10-37 degrees C (optimum 2...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Online Access: | https://hal.science/hal-02372883 https://doi.org/10.1099/ijsem.0.003234 |
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ftunivnantes:oai:HAL:hal-02372883v1 2023-05-15T15:10:26+02:00 Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge Bauer, Sven Le Moine Sjoberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roakvam, Irene Steen, Ida Helene Dahle, Hakon Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) Institute of Theoretical Astrophysics Oslo University of Oslo (UiO) 2019 https://hal.science/hal-02372883 https://doi.org/10.1099/ijsem.0.003234 en eng HAL CCSD Microbiology Society info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.003234 hal-02372883 https://hal.science/hal-02372883 doi:10.1099/ijsem.0.003234 ISSN: 1466-5026 EISSN: 1466-5034 International Journal of Systematic and Evolutionary Microbiology https://hal.science/hal-02372883 International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (4), pp.975-981. ⟨10.1099/ijsem.0.003234⟩ isolate deep-sea hydrothermal vent roseobacter alphaproteobacteria piezophilic [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology info:eu-repo/semantics/article Journal articles 2019 ftunivnantes https://doi.org/10.1099/ijsem.0.003234 2023-01-18T00:52:27Z A bacterial strain, designated BAR1(T), was isolated from a microbial mat growing on the surface of a barite chimney at the Loki's Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1(T) were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10-37 degrees C (optimum 27-35 degrees C), pH 5.5-8.0 (optimum pH 6.5-7.5) and 0.5-5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was 0-10, the fatty acid profile was dominated by C-18(:1)omega 7C (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1(T) clustered together with Rhodobacterales bacterium PRT1, as well as the genera Halocynthiibacter and Pseudohalocynthiibacter in a polyphyletic Glade within the Roseobacter Glade. Several characteristics differentiate strain BAR1(T) from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 degrees C and under anaerobic conditions. Accordingly, strain BAR1(T) is considered to represent a novel genus and species within the Roseobacter Glade, for which the name Profundibacter amoris gen. nov., sp. nov. is proposed. The type strain is Profundibacter amoris BAR1(T) (=JCM 31874(T) =DSM 104147(T)). Article in Journal/Newspaper Arctic Université de Nantes: HAL-UNIV-NANTES Arctic International Journal of Systematic and Evolutionary Microbiology 69 4 975 981 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
isolate deep-sea hydrothermal vent roseobacter alphaproteobacteria piezophilic [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
spellingShingle |
isolate deep-sea hydrothermal vent roseobacter alphaproteobacteria piezophilic [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Bauer, Sven Le Moine Sjoberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roakvam, Irene Steen, Ida Helene Dahle, Hakon Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
topic_facet |
isolate deep-sea hydrothermal vent roseobacter alphaproteobacteria piezophilic [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
description |
A bacterial strain, designated BAR1(T), was isolated from a microbial mat growing on the surface of a barite chimney at the Loki's Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1(T) were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10-37 degrees C (optimum 27-35 degrees C), pH 5.5-8.0 (optimum pH 6.5-7.5) and 0.5-5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was 0-10, the fatty acid profile was dominated by C-18(:1)omega 7C (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1(T) clustered together with Rhodobacterales bacterium PRT1, as well as the genera Halocynthiibacter and Pseudohalocynthiibacter in a polyphyletic Glade within the Roseobacter Glade. Several characteristics differentiate strain BAR1(T) from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 degrees C and under anaerobic conditions. Accordingly, strain BAR1(T) is considered to represent a novel genus and species within the Roseobacter Glade, for which the name Profundibacter amoris gen. nov., sp. nov. is proposed. The type strain is Profundibacter amoris BAR1(T) (=JCM 31874(T) =DSM 104147(T)). |
author2 |
Laboratoire de microbiologie des environnements extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) Institute of Theoretical Astrophysics Oslo University of Oslo (UiO) |
format |
Article in Journal/Newspaper |
author |
Bauer, Sven Le Moine Sjoberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roakvam, Irene Steen, Ida Helene Dahle, Hakon |
author_facet |
Bauer, Sven Le Moine Sjoberg, Andreas Gilje L'Haridon, Stéphane Stokke, Runar Roakvam, Irene Steen, Ida Helene Dahle, Hakon |
author_sort |
Bauer, Sven Le Moine |
title |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_short |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_full |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_fullStr |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_full_unstemmed |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter Glade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_sort |
profundibacter amoris gen. nov., sp. nov., a new member of the roseobacter glade isolated from loki's castle vent field on the arctic mid-ocean ridge |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02372883 https://doi.org/10.1099/ijsem.0.003234 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
ISSN: 1466-5026 EISSN: 1466-5034 International Journal of Systematic and Evolutionary Microbiology https://hal.science/hal-02372883 International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (4), pp.975-981. ⟨10.1099/ijsem.0.003234⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1099/ijsem.0.003234 hal-02372883 https://hal.science/hal-02372883 doi:10.1099/ijsem.0.003234 |
op_doi |
https://doi.org/10.1099/ijsem.0.003234 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
69 |
container_issue |
4 |
container_start_page |
975 |
op_container_end_page |
981 |
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1766341463561469952 |