Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas )
International audience The marine coastal environment is exposed to a mixture of environmental pollutants of anthropogenic origin, resulting in chronic low concentrations of contaminants. As a consequence, most coastal marine species are exposed to low doses of such pollutants during their entire li...
Published in: | Environmental Toxicology and Chemistry |
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Online Access: | https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 https://doi.org/10.1002/etc.2012 |
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ftunivnantes:oai:HAL:hal-02314079v1 2023-05-15T15:58:14+02:00 Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) Devos, Alexandre Voiseux, Claire Caplat, Christelle Fievet, Bruno Laboratoire d'écotoxicologie des radionucléides (IRSN/PRP-ENV/SERIS/LECO) Service de Recherche et d'Expertise sur les Risques environnementaux (IRSN/PRP-ENV/SERIS) Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2012-12 https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 https://doi.org/10.1002/etc.2012 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.2012 hal-02314079 https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 doi:10.1002/etc.2012 ISSN: 0730-7268 EISSN: 1552-8618 Environmental Toxicology and Chemistry https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 Environmental Toxicology and Chemistry, 2012, 31 (12), pp.2841-2847. ⟨10.1002/etc.2012⟩ Zinc Chronic exposure Metallothionein Gene expression Oyster spat [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftunivnantes https://doi.org/10.1002/etc.2012 2023-03-08T05:11:07Z International audience The marine coastal environment is exposed to a mixture of environmental pollutants of anthropogenic origin, resulting in chronic low concentrations of contaminants. As a consequence, most coastal marine species are exposed to low doses of such pollutants during their entire life. Many marine species live for years in their natural environment, whereas they do not under laboratory exposure conditions. Using early stages of development in laboratory work allows animals to be chronically exposed from an early age over a reasonable experiment period. In the present study, the authors investigated the effect of chronic exposure to zinc in spats of the Pacific oyster (Crassostrea gigas), from metamorphosis up to 10 weeks. The authors investigated integrated biological endpoints that would account for the apparent general health of the animals as well as molecular markers showing more subtle effects that could potentially go unnoticed at a biologically integrated level. The authors measured in parallel both growth and the transcriptional level of target stress genes. Growth was monitored by image analysis of large samples to avoid high variability and ensure statistical robustness. A dose–response relationship was derived from growth data, yielding a median effective concentration (EC50) of 7.55 µM. Stress genes selected on the basis of available RNA sequences in C. gigas included genes involved in chaperone proteins, oxidative stress, detoxification, and cell cycle regulation. Out of nine stress target genes, only metallothionein displayed overexpression in response to high levels of zinc. Environ. Toxicol. Chem. 2012; 31: 2841–2847. © 2012 SETAC Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Environmental Toxicology and Chemistry 31 12 2841 2847 |
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Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Zinc Chronic exposure Metallothionein Gene expression Oyster spat [SDE]Environmental Sciences |
spellingShingle |
Zinc Chronic exposure Metallothionein Gene expression Oyster spat [SDE]Environmental Sciences Devos, Alexandre Voiseux, Claire Caplat, Christelle Fievet, Bruno Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) |
topic_facet |
Zinc Chronic exposure Metallothionein Gene expression Oyster spat [SDE]Environmental Sciences |
description |
International audience The marine coastal environment is exposed to a mixture of environmental pollutants of anthropogenic origin, resulting in chronic low concentrations of contaminants. As a consequence, most coastal marine species are exposed to low doses of such pollutants during their entire life. Many marine species live for years in their natural environment, whereas they do not under laboratory exposure conditions. Using early stages of development in laboratory work allows animals to be chronically exposed from an early age over a reasonable experiment period. In the present study, the authors investigated the effect of chronic exposure to zinc in spats of the Pacific oyster (Crassostrea gigas), from metamorphosis up to 10 weeks. The authors investigated integrated biological endpoints that would account for the apparent general health of the animals as well as molecular markers showing more subtle effects that could potentially go unnoticed at a biologically integrated level. The authors measured in parallel both growth and the transcriptional level of target stress genes. Growth was monitored by image analysis of large samples to avoid high variability and ensure statistical robustness. A dose–response relationship was derived from growth data, yielding a median effective concentration (EC50) of 7.55 µM. Stress genes selected on the basis of available RNA sequences in C. gigas included genes involved in chaperone proteins, oxidative stress, detoxification, and cell cycle regulation. Out of nine stress target genes, only metallothionein displayed overexpression in response to high levels of zinc. Environ. Toxicol. Chem. 2012; 31: 2841–2847. © 2012 SETAC |
author2 |
Laboratoire d'écotoxicologie des radionucléides (IRSN/PRP-ENV/SERIS/LECO) Service de Recherche et d'Expertise sur les Risques environnementaux (IRSN/PRP-ENV/SERIS) Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Devos, Alexandre Voiseux, Claire Caplat, Christelle Fievet, Bruno |
author_facet |
Devos, Alexandre Voiseux, Claire Caplat, Christelle Fievet, Bruno |
author_sort |
Devos, Alexandre |
title |
Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) |
title_short |
Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) |
title_full |
Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) |
title_fullStr |
Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) |
title_full_unstemmed |
Effect of chronic exposure to zinc in young spats of the Pacific oyster ( Crassostrea gigas ) |
title_sort |
effect of chronic exposure to zinc in young spats of the pacific oyster ( crassostrea gigas ) |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 https://doi.org/10.1002/etc.2012 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0730-7268 EISSN: 1552-8618 Environmental Toxicology and Chemistry https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 Environmental Toxicology and Chemistry, 2012, 31 (12), pp.2841-2847. ⟨10.1002/etc.2012⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1002/etc.2012 hal-02314079 https://hal-normandie-univ.archives-ouvertes.fr/hal-02314079 doi:10.1002/etc.2012 |
op_doi |
https://doi.org/10.1002/etc.2012 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
31 |
container_issue |
12 |
container_start_page |
2841 |
op_container_end_page |
2847 |
_version_ |
1766393963912101888 |