Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells
International audience Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae (V. crassostreae) has been repeatedly isolated from diseased animals, and the majority of the...
Published in: | Environmental Microbiology |
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Main Authors: | , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2019
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Subjects: | |
Online Access: | https://hal.sorbonne-universite.fr/hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754/document https://hal.sorbonne-universite.fr/hal-02298754/file/Piel%20et%20al.%20-%20Selection%20of%20Vibrio%20crassostreae%20relies%20on%20a%20plasm.pdf https://doi.org/10.1111/1462-2920.14776 |
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institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
TREE ACL SEQUENCE FAMILY SPLENDIDUS MORTALITY PATHOGEN [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
TREE ACL SEQUENCE FAMILY SPLENDIDUS MORTALITY PATHOGEN [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Mathias Wegner, K Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
topic_facet |
TREE ACL SEQUENCE FAMILY SPLENDIDUS MORTALITY PATHOGEN [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae (V. crassostreae) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. In this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae, while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a type 6 secretion system (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause haemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams respectively. We propose that haemocyte cytotoxicity is a lethality trait shared by a broad range of mollusc pathogens, and we speculate that T6SS was involved in parallel evolution of pathogen for molluscs. |
author2 |
Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Alfred Wegener Institute Potsdam Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) Université de Lausanne = University of Lausanne (UNIL) Ecole Polytechnique Fédérale de Lausanne (EPFL) ANR-16-CE32-0008,REVENGE,L'huître comme niche de l'évolution et l'émergence de vibrios pathogènes(2016) |
format |
Article in Journal/Newspaper |
author |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Mathias Wegner, K Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique |
author_facet |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Mathias Wegner, K Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique |
author_sort |
Piel, Damien |
title |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_short |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_fullStr |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full_unstemmed |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_sort |
selection of vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.sorbonne-universite.fr/hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754/document https://hal.sorbonne-universite.fr/hal-02298754/file/Piel%20et%20al.%20-%20Selection%20of%20Vibrio%20crassostreae%20relies%20on%20a%20plasm.pdf https://doi.org/10.1111/1462-2920.14776 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.sorbonne-universite.fr/hal-02298754 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, ⟨10.1111/1462-2920.14776⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.14776 hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754/document https://hal.sorbonne-universite.fr/hal-02298754/file/Piel%20et%20al.%20-%20Selection%20of%20Vibrio%20crassostreae%20relies%20on%20a%20plasm.pdf doi:10.1111/1462-2920.14776 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/1462-2920.14776 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
10 |
container_start_page |
4198 |
op_container_end_page |
4211 |
_version_ |
1766394502411452416 |
spelling |
ftunivnantes:oai:HAL:hal-02298754v1 2023-05-15T15:58:44+02:00 Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Mathias Wegner, K Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Alfred Wegener Institute Potsdam Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) Université de Lausanne = University of Lausanne (UNIL) Ecole Polytechnique Fédérale de Lausanne (EPFL) ANR-16-CE32-0008,REVENGE,L'huître comme niche de l'évolution et l'émergence de vibrios pathogènes(2016) 2019-08-26 https://hal.sorbonne-universite.fr/hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754/document https://hal.sorbonne-universite.fr/hal-02298754/file/Piel%20et%20al.%20-%20Selection%20of%20Vibrio%20crassostreae%20relies%20on%20a%20plasm.pdf https://doi.org/10.1111/1462-2920.14776 en eng HAL CCSD Society for Applied Microbiology and Wiley-Blackwell info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.14776 hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754 https://hal.sorbonne-universite.fr/hal-02298754/document https://hal.sorbonne-universite.fr/hal-02298754/file/Piel%20et%20al.%20-%20Selection%20of%20Vibrio%20crassostreae%20relies%20on%20a%20plasm.pdf doi:10.1111/1462-2920.14776 info:eu-repo/semantics/OpenAccess ISSN: 1462-2912 EISSN: 1462-2920 Environmental Microbiology https://hal.sorbonne-universite.fr/hal-02298754 Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2019, ⟨10.1111/1462-2920.14776⟩ TREE ACL SEQUENCE FAMILY SPLENDIDUS MORTALITY PATHOGEN [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2019 ftunivnantes https://doi.org/10.1111/1462-2920.14776 2022-09-07T00:02:02Z International audience Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae (V. crassostreae) has been repeatedly isolated from diseased animals, and the majority of the strains have been demonstrated to be virulent for oysters. In this study, we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae, while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a type 6 secretion system (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause haemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams respectively. We propose that haemocyte cytotoxicity is a lethality trait shared by a broad range of mollusc pathogens, and we speculate that T6SS was involved in parallel evolution of pathogen for molluscs. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Environmental Microbiology 22 10 4198 4211 |