Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos
International audience Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely u...
Published in: | Aquatic Toxicology |
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Online Access: | https://hal.archives-ouvertes.fr/hal-02155835 https://doi.org/10.1016/j.aquatox.2018.01.001 |
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ftunivnantes:oai:HAL:hal-02155835v1 2023-05-15T15:58:25+02:00 Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos Sussarellu, Rossana Lebreton, Morgane Rouxel, Julien Akcha, Farida Rivière, Guillaume Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) 2018-03 https://hal.archives-ouvertes.fr/hal-02155835 https://doi.org/10.1016/j.aquatox.2018.01.001 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2018.01.001 hal-02155835 https://hal.archives-ouvertes.fr/hal-02155835 doi:10.1016/j.aquatox.2018.01.001 ISSN: 0166-445X Aquatic Toxicology https://hal.archives-ouvertes.fr/hal-02155835 Aquatic Toxicology, Elsevier, 2018, 196, pp.70-78. ⟨10.1016/j.aquatox.2018.01.001⟩ Oyster Embryotoxicity Copper Gene expression DNA methylation [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2018 ftunivnantes https://doi.org/10.1016/j.aquatox.2018.01.001 2022-07-13T00:16:54Z International audience Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development.For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval bioassay at the D-larva stage 24 h post fertilization (hpf) and genotoxicity at gastrulation 7 hpf. In parallel, the relative expression of 15 genes encoding putative homeotic, biomineralization and DNA methylation proteins was measured at three developmental stages (3 hpf morula stage, 7 hpf gastrula stage, 24 hpf D-larvae stage) using RT-qPCR. Global DNA content in methylcytosine and hydroxymethylcytosine were measured by HPLC and gene-specific DNA methylation levels were monitored using MeDIP-qPCR.A significant increase in larval abnormalities was observed from copper concentrations of 10 μg L−1, while significant genotoxic effects were detected at 1 μg L−1 and above. All the selected genes presented a stage-dependent expression pattern, which was impaired for some homeobox and DNA methylation genes (Notochord, HOXA1, HOX2, Lox5, DNMT3b and CXXC-1) after copper exposure. While global DNA methylation (5-methylcytosine) at gastrula stage didn’t show significant changes between experimental conditions, 5-hydroxymethylcytosine, its degradation product, decreased upon copper treatment. The DNA methylation of exons and the transcript levels were correlated in control samples for HOXA1 but such a correlation was diminished following copper ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Aquatic Toxicology 196 70 78 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Oyster Embryotoxicity Copper Gene expression DNA methylation [SDV]Life Sciences [q-bio] |
spellingShingle |
Oyster Embryotoxicity Copper Gene expression DNA methylation [SDV]Life Sciences [q-bio] Sussarellu, Rossana Lebreton, Morgane Rouxel, Julien Akcha, Farida Rivière, Guillaume Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos |
topic_facet |
Oyster Embryotoxicity Copper Gene expression DNA methylation [SDV]Life Sciences [q-bio] |
description |
International audience Copper contamination is widespread along coastal areas and exerts adverse effects on marine organisms such as mollusks. In the Pacific oyster, copper induces severe developmental abnormalities during early life stages; however, the underlying molecular mechanisms are largely unknown. This study aims to better understand whether the embryotoxic effects of copper in Crassostrea gigas could be mediated by alterations in gene expression, and the putative role of DNA methylation, which is known to contribute to gene regulation in early embryo development.For that purpose, oyster embryos were exposed to 4 nominal copper concentrations (0.1, 1, 10 and 20 μg L−1 Cu2+) during early development assays. Embryotoxicity was monitored through the oyster embryo-larval bioassay at the D-larva stage 24 h post fertilization (hpf) and genotoxicity at gastrulation 7 hpf. In parallel, the relative expression of 15 genes encoding putative homeotic, biomineralization and DNA methylation proteins was measured at three developmental stages (3 hpf morula stage, 7 hpf gastrula stage, 24 hpf D-larvae stage) using RT-qPCR. Global DNA content in methylcytosine and hydroxymethylcytosine were measured by HPLC and gene-specific DNA methylation levels were monitored using MeDIP-qPCR.A significant increase in larval abnormalities was observed from copper concentrations of 10 μg L−1, while significant genotoxic effects were detected at 1 μg L−1 and above. All the selected genes presented a stage-dependent expression pattern, which was impaired for some homeobox and DNA methylation genes (Notochord, HOXA1, HOX2, Lox5, DNMT3b and CXXC-1) after copper exposure. While global DNA methylation (5-methylcytosine) at gastrula stage didn’t show significant changes between experimental conditions, 5-hydroxymethylcytosine, its degradation product, decreased upon copper treatment. The DNA methylation of exons and the transcript levels were correlated in control samples for HOXA1 but such a correlation was diminished following copper ... |
author2 |
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) |
format |
Article in Journal/Newspaper |
author |
Sussarellu, Rossana Lebreton, Morgane Rouxel, Julien Akcha, Farida Rivière, Guillaume |
author_facet |
Sussarellu, Rossana Lebreton, Morgane Rouxel, Julien Akcha, Farida Rivière, Guillaume |
author_sort |
Sussarellu, Rossana |
title |
Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos |
title_short |
Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos |
title_full |
Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos |
title_fullStr |
Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos |
title_full_unstemmed |
Copper induces expression and methylation changes of early development genes in Crassostrea gigas embryos |
title_sort |
copper induces expression and methylation changes of early development genes in crassostrea gigas embryos |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-02155835 https://doi.org/10.1016/j.aquatox.2018.01.001 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.archives-ouvertes.fr/hal-02155835 Aquatic Toxicology, Elsevier, 2018, 196, pp.70-78. ⟨10.1016/j.aquatox.2018.01.001⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2018.01.001 hal-02155835 https://hal.archives-ouvertes.fr/hal-02155835 doi:10.1016/j.aquatox.2018.01.001 |
op_doi |
https://doi.org/10.1016/j.aquatox.2018.01.001 |
container_title |
Aquatic Toxicology |
container_volume |
196 |
container_start_page |
70 |
op_container_end_page |
78 |
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1766394159275442176 |