Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles
International audience The study tests the physiological responses of Persian sturgeon, Acipenser persicus, during the abrupt release of juveniles from freshwater (FW) into brackish waters (BW=11 parts per thousand) of the Caspian Sea. Fish weight at release was 2-3g (2.55 +/- 0.41g; 8.8 +/- 0.58cm...
Published in: | Journal of Applied Ichthyology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.umontpellier.fr/hal-01927985 https://doi.org/10.1111/jai.13260 |
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ftunivnantes:oai:HAL:hal-01927985v1 2023-05-15T18:09:59+02:00 Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles Shirangi, S. Kalbassi, M. Khodabandeh, S. Jafarian, H. Lignot, J.-H. MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) 2017-06 https://hal.umontpellier.fr/hal-01927985 https://doi.org/10.1111/jai.13260 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/jai.13260 hal-01927985 https://hal.umontpellier.fr/hal-01927985 doi:10.1111/jai.13260 ISSN: 0175-8659 EISSN: 1439-0426 Journal of Applied Ichthyology https://hal.umontpellier.fr/hal-01927985 Journal of Applied Ichthyology, 2017, 33 (3), pp.347 - 353. ⟨10.1111/jai.13260⟩ acclimation bass dicentrarchus-labrax cftr anion channel fish gene-expression na+ k+ 2cl(-) cotransporter k+-atpase Osmoregulation rich cells salmon salmo-salar [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems info:eu-repo/semantics/article Journal articles 2017 ftunivnantes https://doi.org/10.1111/jai.13260 2023-02-08T08:13:38Z International audience The study tests the physiological responses of Persian sturgeon, Acipenser persicus, during the abrupt release of juveniles from freshwater (FW) into brackish waters (BW=11 parts per thousand) of the Caspian Sea. Fish weight at release was 2-3g (2.55 +/- 0.41g; 8.8 +/- 0.58cm TL). Totals of 160 individuals were randomly distributed into four fiber-glass aerated tanks (volume 60-L). Two tanks served as controls (FW groups), and two as exposure tanks for BW (Caspian Sea water=CSW). Fish were sampled at 0, 3, 6, 12, 24, 48 and 96hr after abrupt transfer to CSW. Plasma osmolality, immunolocalization of Na+, K+ -ATPase (NKA) and Na+/K+/2Cl(-) (NKCC) Co-transporter, NKA activity and the NKA -subunit mRNA expression were analyzed. Blood osmolality of fish transferred from FW to CSW increased significantly within hours post-transfer (p<.05) and remained at a high level for up to 96hr. Immunolocalization of NKCC indicated co-localization with NKA in the chloride cells in the gill epithelium. A partial sequence of the NKA -subunit (632bp) is described. Its expression levels were up-regulated at 12 and 48hr following salinity transfer (p<.05). However, NKA activity sharply increased in CSW specimens by almost 2.8-fold (p<.05) between 48 and 96hr after transfer. Gill NKCC co-transporter abundance increased, coinciding with increased gill NKA activity. The increased activity of NKCC during salt excretion in CSW may lead to an influx of Na+ into the chloride cells. Consequently, NKA activity increases to maintain intracellular Na+ homeostasis. Article in Journal/Newspaper Salmo salar Université de Nantes: HAL-UNIV-NANTES Borodin ENVELOPE(-72.627,-72.627,-71.603,-71.603) Journal of Applied Ichthyology 33 3 347 353 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
acclimation bass dicentrarchus-labrax cftr anion channel fish gene-expression na+ k+ 2cl(-) cotransporter k+-atpase Osmoregulation rich cells salmon salmo-salar [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems |
spellingShingle |
acclimation bass dicentrarchus-labrax cftr anion channel fish gene-expression na+ k+ 2cl(-) cotransporter k+-atpase Osmoregulation rich cells salmon salmo-salar [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems Shirangi, S. Kalbassi, M. Khodabandeh, S. Jafarian, H. Lignot, J.-H. Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles |
topic_facet |
acclimation bass dicentrarchus-labrax cftr anion channel fish gene-expression na+ k+ 2cl(-) cotransporter k+-atpase Osmoregulation rich cells salmon salmo-salar [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDE.MCG]Environmental Sciences/Global Changes [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems |
description |
International audience The study tests the physiological responses of Persian sturgeon, Acipenser persicus, during the abrupt release of juveniles from freshwater (FW) into brackish waters (BW=11 parts per thousand) of the Caspian Sea. Fish weight at release was 2-3g (2.55 +/- 0.41g; 8.8 +/- 0.58cm TL). Totals of 160 individuals were randomly distributed into four fiber-glass aerated tanks (volume 60-L). Two tanks served as controls (FW groups), and two as exposure tanks for BW (Caspian Sea water=CSW). Fish were sampled at 0, 3, 6, 12, 24, 48 and 96hr after abrupt transfer to CSW. Plasma osmolality, immunolocalization of Na+, K+ -ATPase (NKA) and Na+/K+/2Cl(-) (NKCC) Co-transporter, NKA activity and the NKA -subunit mRNA expression were analyzed. Blood osmolality of fish transferred from FW to CSW increased significantly within hours post-transfer (p<.05) and remained at a high level for up to 96hr. Immunolocalization of NKCC indicated co-localization with NKA in the chloride cells in the gill epithelium. A partial sequence of the NKA -subunit (632bp) is described. Its expression levels were up-regulated at 12 and 48hr following salinity transfer (p<.05). However, NKA activity sharply increased in CSW specimens by almost 2.8-fold (p<.05) between 48 and 96hr after transfer. Gill NKCC co-transporter abundance increased, coinciding with increased gill NKA activity. The increased activity of NKCC during salt excretion in CSW may lead to an influx of Na+ into the chloride cells. Consequently, NKA activity increases to maintain intracellular Na+ homeostasis. |
author2 |
MARine Biodiversity Exploitation and Conservation (UMR MARBEC) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Shirangi, S. Kalbassi, M. Khodabandeh, S. Jafarian, H. Lignot, J.-H. |
author_facet |
Shirangi, S. Kalbassi, M. Khodabandeh, S. Jafarian, H. Lignot, J.-H. |
author_sort |
Shirangi, S. |
title |
Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles |
title_short |
Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles |
title_full |
Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles |
title_fullStr |
Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles |
title_full_unstemmed |
Immunolocalization of Na + /K + -ATPase, Na + /K + /2Cl − co-transporter (NKCC) and mRNA expression of Na + /K + -ATPase α-subunit during short-term salinity transfer in the gills of Persian sturgeon ( Acipenser persicus , Borodin, 1897) juveniles |
title_sort |
immunolocalization of na + /k + -atpase, na + /k + /2cl − co-transporter (nkcc) and mrna expression of na + /k + -atpase α-subunit during short-term salinity transfer in the gills of persian sturgeon ( acipenser persicus , borodin, 1897) juveniles |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.umontpellier.fr/hal-01927985 https://doi.org/10.1111/jai.13260 |
long_lat |
ENVELOPE(-72.627,-72.627,-71.603,-71.603) |
geographic |
Borodin |
geographic_facet |
Borodin |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
ISSN: 0175-8659 EISSN: 1439-0426 Journal of Applied Ichthyology https://hal.umontpellier.fr/hal-01927985 Journal of Applied Ichthyology, 2017, 33 (3), pp.347 - 353. ⟨10.1111/jai.13260⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/jai.13260 hal-01927985 https://hal.umontpellier.fr/hal-01927985 doi:10.1111/jai.13260 |
op_doi |
https://doi.org/10.1111/jai.13260 |
container_title |
Journal of Applied Ichthyology |
container_volume |
33 |
container_issue |
3 |
container_start_page |
347 |
op_container_end_page |
353 |
_version_ |
1766182695894777856 |