Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi
International audience Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affe...
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ftunivnantes:oai:HAL:hal-01852344v1 2023-05-15T15:48:01+02:00 Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi Amato, Alberto Sabatino, Valeria Nylund, Göran Bergkvist, Johanna Basu, Swaraj Andersson, Mats Sanges, Remo Godhe, Anna Kiorboe, Thomas Selander, Erik Ferrante, Maria Physiologie cellulaire et végétale (LPCV) Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Integrative Marine Ecology Stazione Zoologica Anton Dohrn (SZN) Department of Marine Sciences Gothenburg University of Gothenburg (GU) Department of Biological and Environmental Sciences Gothenburg National Institute of Aquatic Resources Danmarks Tekniske Universitet = Technical University of Denmark (DTU) Swedish Research Council VR 2015-05491; European Union under FP7-People 600407; Swedish Research Council Formas VR 217-2008-1719 European Project: 227799,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,ASSEMBLE(2009) 2018-06 https://hal.archives-ouvertes.fr/hal-01852344 https://doi.org/10.1038/s41396-018-0094-0 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41396-018-0094-0 info:eu-repo/semantics/altIdentifier/pmid/29599523 info:eu-repo/grantAgreement/EC/FP7/227799/EU/Association of European Marine Biological Laboratories/ASSEMBLE hal-01852344 https://hal.archives-ouvertes.fr/hal-01852344 doi:10.1038/s41396-018-0094-0 PRODINRA: 448658 PUBMED: 29599523 PUBMEDCENTRAL: PMC5955879 WOS: 000432357500017 ISSN: 1751-7362 EISSN: 1751-7370 ISME Journal https://hal.archives-ouvertes.fr/hal-01852344 ISME Journal, Nature Publishing Group, 2018, 12 (6), pp.1594 - 1604. ⟨10.1038/s41396-018-0094-0⟩ [SDV]Life Sciences [q-bio] [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology info:eu-repo/semantics/article Journal articles 2018 ftunivnantes https://doi.org/10.1038/s41396-018-0094-0 2022-08-10T06:09:14Z International audience Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems. Article in Journal/Newspaper Calanus finmarchicus Copepods Université de Nantes: HAL-UNIV-NANTES The ISME Journal 12 6 1594 1604 |
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Open Polar |
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Université de Nantes: HAL-UNIV-NANTES |
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ftunivnantes |
language |
English |
topic |
[SDV]Life Sciences [q-bio] [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
spellingShingle |
[SDV]Life Sciences [q-bio] [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amato, Alberto Sabatino, Valeria Nylund, Göran Bergkvist, Johanna Basu, Swaraj Andersson, Mats Sanges, Remo Godhe, Anna Kiorboe, Thomas Selander, Erik Ferrante, Maria Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
topic_facet |
[SDV]Life Sciences [q-bio] [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology |
description |
International audience Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems. |
author2 |
Physiologie cellulaire et végétale (LPCV) Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG) Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Integrative Marine Ecology Stazione Zoologica Anton Dohrn (SZN) Department of Marine Sciences Gothenburg University of Gothenburg (GU) Department of Biological and Environmental Sciences Gothenburg National Institute of Aquatic Resources Danmarks Tekniske Universitet = Technical University of Denmark (DTU) Swedish Research Council VR 2015-05491; European Union under FP7-People 600407; Swedish Research Council Formas VR 217-2008-1719 European Project: 227799,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,ASSEMBLE(2009) |
format |
Article in Journal/Newspaper |
author |
Amato, Alberto Sabatino, Valeria Nylund, Göran Bergkvist, Johanna Basu, Swaraj Andersson, Mats Sanges, Remo Godhe, Anna Kiorboe, Thomas Selander, Erik Ferrante, Maria |
author_facet |
Amato, Alberto Sabatino, Valeria Nylund, Göran Bergkvist, Johanna Basu, Swaraj Andersson, Mats Sanges, Remo Godhe, Anna Kiorboe, Thomas Selander, Erik Ferrante, Maria |
author_sort |
Amato, Alberto |
title |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_short |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_full |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_fullStr |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_full_unstemmed |
Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi |
title_sort |
grazer-induced transcriptomic and metabolomic response of the chain-forming diatom skeletonema marinoi |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.archives-ouvertes.fr/hal-01852344 https://doi.org/10.1038/s41396-018-0094-0 |
genre |
Calanus finmarchicus Copepods |
genre_facet |
Calanus finmarchicus Copepods |
op_source |
ISSN: 1751-7362 EISSN: 1751-7370 ISME Journal https://hal.archives-ouvertes.fr/hal-01852344 ISME Journal, Nature Publishing Group, 2018, 12 (6), pp.1594 - 1604. ⟨10.1038/s41396-018-0094-0⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41396-018-0094-0 info:eu-repo/semantics/altIdentifier/pmid/29599523 info:eu-repo/grantAgreement/EC/FP7/227799/EU/Association of European Marine Biological Laboratories/ASSEMBLE hal-01852344 https://hal.archives-ouvertes.fr/hal-01852344 doi:10.1038/s41396-018-0094-0 PRODINRA: 448658 PUBMED: 29599523 PUBMEDCENTRAL: PMC5955879 WOS: 000432357500017 |
op_doi |
https://doi.org/10.1038/s41396-018-0094-0 |
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The ISME Journal |
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12 |
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6 |
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