Remodeling of the cycling transcriptome of the oyster Crassostrea gigas by the harmful algae Alexandrium minutum

• Published in: Scientific Reports
• Main Authors: Payton, Laura, Perrigault, Mickael, Hoede, Claire, Massabuau, Jean-Charles, Sow, Mohamedou, Huvet, Arnaud, Boullot, Floriane, Fabioux, Caroline, Hegaret, Helene, Tran, Damien
• Other Authors: Centre National de la Recherche Scientifique (CNRS), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRA), Institut National de la Recherche Agronomique (INRA), Plateforme Bio-Informatique - Génotoul, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), ANR ACCUTOX 13-CESA-0019
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2017
• Subjects: MAMMALIAN CIRCADIAN CLOCK; DIFFERENTIAL EXPRESSION ANALYSIS; PARALYTIC SHELLFISH TOXINS; GENE-EXPRESSION; SUPRACHIASMATIC NUCLEUS; FEEDING RESPONSES; MOUSE-LIVER; SHIFT WORK; DINOFLAGELLATE; RHYTHMS; [SDV]Life Sciences [q-bio]; Crassostrea gigas
• Online Access: https://hal.archives-ouvertes.fr/hal-01605998; https://hal.archives-ouvertes.fr/hal-01605998/document; https://hal.archives-ouvertes.fr/hal-01605998/file/Remodeling%20of%20the%20cycling%20transcriptome%20of%20the%20oyster%20Crassostrea%20gigas%20by%20the%20harmful%20algae%20Alexandrium%20minutum_1.pdf; https://doi.org/10.1038/s41598-017-03797-4

As a marine organism, the oyster Crassostrea gigas inhabits a complex biotope governed by interactions between the moon and the sun cycles. We used next-generation sequencing to investigate temporal regulation of oysters under light/dark entrainment and the impact of harmful algal exposure. We found that approximate to 6% of the gills' transcriptome exhibits circadian expression, characterized by a nocturnal and bimodal pattern. Surprisingly, a higher number of ultradian transcripts were also detected under solely circadian entrainment. The results showed that a bloom of Alexandrium minutum generated a remodeling of the bivalve's temporal structure, characterized by a loss of oscillations, a genesis of de novo oscillating transcripts, and a switch in the period of oscillations. These findings provide unprecedented insights into the diurnal landscape of the oyster's transcriptome and pleiotropic remodeling due to toxic algae exposure, revealing the intrinsic plasticity of the cycling transcriptome in oysters.