The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway
Article accepté Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemoc...
Published in: | Aquatic Toxicology |
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Format: | Article in Journal/Newspaper |
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Online Access: | https://hal.science/hal-01561503 https://hal.science/hal-01561503/document https://hal.science/hal-01561503/file/Abi-KhalilC.-et%20al-PrePrint-AquaticToxico.-2017_%7B5391CD15-5587-4D24-A8CB-EEE879DFA9DB%7D.pdf https://doi.org/10.1016/j.aquatox.2017.07.001 |
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ftunivnantes:oai:HAL:hal-01561503v1 2023-05-15T15:57:59+02:00 The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine Rolland, Jean Luc Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Department of Chemistry Biochimie et Physiologie Moléculaire des Plantes (BPMP) Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) 2017 https://hal.science/hal-01561503 https://hal.science/hal-01561503/document https://hal.science/hal-01561503/file/Abi-KhalilC.-et%20al-PrePrint-AquaticToxico.-2017_%7B5391CD15-5587-4D24-A8CB-EEE879DFA9DB%7D.pdf https://doi.org/10.1016/j.aquatox.2017.07.001 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2017.07.001 hal-01561503 https://hal.science/hal-01561503 https://hal.science/hal-01561503/document https://hal.science/hal-01561503/file/Abi-KhalilC.-et%20al-PrePrint-AquaticToxico.-2017_%7B5391CD15-5587-4D24-A8CB-EEE879DFA9DB%7D.pdf doi:10.1016/j.aquatox.2017.07.001 PRODINRA: 398796 WOS: 000408783600016 http://creativecommons.org/licenses/by-sa/ info:eu-repo/semantics/OpenAccess CC-BY-SA ISSN: 0166-445X Aquatic Toxicology https://hal.science/hal-01561503 Aquatic Toxicology, 2017, 190, ⟨10.1016/j.aquatox.2017.07.001⟩ Shellfish Toxin Harmful algae Phytoplankton crassostrea gigas hémocyte réaction hémocytaire [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2017 ftunivnantes https://doi.org/10.1016/j.aquatox.2017.07.001 2023-03-01T05:47:11Z Article accepté Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which ispresent in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalvemolluscs to microbial infection. Article in Journal/Newspaper Crassostrea gigas Université de Nantes: HAL-UNIV-NANTES Aquatic Toxicology 190 133 141 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Shellfish Toxin Harmful algae Phytoplankton crassostrea gigas hémocyte réaction hémocytaire [SDV]Life Sciences [q-bio] |
spellingShingle |
Shellfish Toxin Harmful algae Phytoplankton crassostrea gigas hémocyte réaction hémocytaire [SDV]Life Sciences [q-bio] Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine Rolland, Jean Luc The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
topic_facet |
Shellfish Toxin Harmful algae Phytoplankton crassostrea gigas hémocyte réaction hémocytaire [SDV]Life Sciences [q-bio] |
description |
Article accepté Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which ispresent in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalvemolluscs to microbial infection. |
author2 |
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Department of Chemistry Biochimie et Physiologie Moléculaire des Plantes (BPMP) Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Article in Journal/Newspaper |
author |
Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine Rolland, Jean Luc |
author_facet |
Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine Rolland, Jean Luc |
author_sort |
Abi-Khalil, Celina |
title |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_short |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_full |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_fullStr |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_full_unstemmed |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_sort |
paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-01561503 https://hal.science/hal-01561503/document https://hal.science/hal-01561503/file/Abi-KhalilC.-et%20al-PrePrint-AquaticToxico.-2017_%7B5391CD15-5587-4D24-A8CB-EEE879DFA9DB%7D.pdf https://doi.org/10.1016/j.aquatox.2017.07.001 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.science/hal-01561503 Aquatic Toxicology, 2017, 190, ⟨10.1016/j.aquatox.2017.07.001⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2017.07.001 hal-01561503 https://hal.science/hal-01561503 https://hal.science/hal-01561503/document https://hal.science/hal-01561503/file/Abi-KhalilC.-et%20al-PrePrint-AquaticToxico.-2017_%7B5391CD15-5587-4D24-A8CB-EEE879DFA9DB%7D.pdf doi:10.1016/j.aquatox.2017.07.001 PRODINRA: 398796 WOS: 000408783600016 |
op_rights |
http://creativecommons.org/licenses/by-sa/ info:eu-repo/semantics/OpenAccess |
op_rightsnorm |
CC-BY-SA |
op_doi |
https://doi.org/10.1016/j.aquatox.2017.07.001 |
container_title |
Aquatic Toxicology |
container_volume |
190 |
container_start_page |
133 |
op_container_end_page |
141 |
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1766393694837014528 |