An alternative method for correcting fluorescence quenching
International audience Under high light intensity, phytoplankton protecttheir photosystems from bleaching through nonphotochemicalquenching processes. The consequence ofthis is suppression of fluorescence emission, which mustbe corrected when measuring in situ yield with fluorometers.We present data...
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ftunivnantes:oai:HAL:hal-01293313v1 2023-05-15T18:25:33+02:00 An alternative method for correcting fluorescence quenching Biermann, L. Guinet, C. Bester, M. Brierley, A. Boehme, L. Sea Mammal Research Unit University of St Andrews (SMRU) School of Biology University of St Andrews University of St Andrews Scotland -University of St Andrews Scotland -Natural Environment Research Council (NERC) Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Department of Zoology and Entomology, Mammal Research Institute University of Pretoria South Africa Pelagic Ecology Research Group, Scottish Oceans Institut 2015 https://hal.archives-ouvertes.fr/hal-01293313 https://doi.org/10.5194/os-11-83-2015 en eng HAL CCSD European Geosciences Union info:eu-repo/semantics/altIdentifier/doi/10.5194/os-11-83-2015 hal-01293313 https://hal.archives-ouvertes.fr/hal-01293313 doi:10.5194/os-11-83-2015 ISSN: 1812-0784 EISSN: 1812-0792 Ocean Science https://hal.archives-ouvertes.fr/hal-01293313 Ocean Science, 2015, 11 (1), pp.83-91. ⟨10.5194/os-11-83-2015⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2015 ftunivnantes https://doi.org/10.5194/os-11-83-2015 2023-01-04T00:07:19Z International audience Under high light intensity, phytoplankton protecttheir photosystems from bleaching through nonphotochemicalquenching processes. The consequence ofthis is suppression of fluorescence emission, which mustbe corrected when measuring in situ yield with fluorometers.We present data from the Southern Ocean, collectedover five austral summers by 19 southern elephant sealstagged with fluorometers. Conventionally, fluorescence datacollected during the day (quenched) were corrected using thelimit of the mixed layer, assuming that phytoplankton areuniformly mixed from the surface to this depth. However,distinct deep fluorescence maxima were measured in approximately30% of the night (unquenched) data. To account forthe evidence that chlorophyll is not uniformly mixed in theupper layer, we propose correcting from the limit of the euphoticzone, defined as the depth at which photosyntheticallyavailable radiation is 1%of the surface value. Mixed layerdepth exceeded euphotic depth over 80% of the time. Underthese conditions, quenching was corrected from the depth ofthe remotely derived euphotic zone Zeu, and compared withfluorescence corrected from the depth of the density-derivedmixed layer. Deep fluorescence maxima were evident in only10%of the day data when correcting from mixed layer depth.This was doubled to 21% when correcting from Zeu, moreclosely matching the unquenched (night) data. Furthermore,correcting from Zeu served to conserve non-uniform chlorophyllfeatures found between the 1% light level and mixedlayer depth. Article in Journal/Newspaper Southern Ocean Université de Nantes: HAL-UNIV-NANTES Austral Southern Ocean Ocean Science 11 1 83 91 |
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Université de Nantes: HAL-UNIV-NANTES |
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English |
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[SDE]Environmental Sciences |
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[SDE]Environmental Sciences Biermann, L. Guinet, C. Bester, M. Brierley, A. Boehme, L. An alternative method for correcting fluorescence quenching |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience Under high light intensity, phytoplankton protecttheir photosystems from bleaching through nonphotochemicalquenching processes. The consequence ofthis is suppression of fluorescence emission, which mustbe corrected when measuring in situ yield with fluorometers.We present data from the Southern Ocean, collectedover five austral summers by 19 southern elephant sealstagged with fluorometers. Conventionally, fluorescence datacollected during the day (quenched) were corrected using thelimit of the mixed layer, assuming that phytoplankton areuniformly mixed from the surface to this depth. However,distinct deep fluorescence maxima were measured in approximately30% of the night (unquenched) data. To account forthe evidence that chlorophyll is not uniformly mixed in theupper layer, we propose correcting from the limit of the euphoticzone, defined as the depth at which photosyntheticallyavailable radiation is 1%of the surface value. Mixed layerdepth exceeded euphotic depth over 80% of the time. Underthese conditions, quenching was corrected from the depth ofthe remotely derived euphotic zone Zeu, and compared withfluorescence corrected from the depth of the density-derivedmixed layer. Deep fluorescence maxima were evident in only10%of the day data when correcting from mixed layer depth.This was doubled to 21% when correcting from Zeu, moreclosely matching the unquenched (night) data. Furthermore,correcting from Zeu served to conserve non-uniform chlorophyllfeatures found between the 1% light level and mixedlayer depth. |
author2 |
Sea Mammal Research Unit University of St Andrews (SMRU) School of Biology University of St Andrews University of St Andrews Scotland -University of St Andrews Scotland -Natural Environment Research Council (NERC) Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Department of Zoology and Entomology, Mammal Research Institute University of Pretoria South Africa Pelagic Ecology Research Group, Scottish Oceans Institut |
format |
Article in Journal/Newspaper |
author |
Biermann, L. Guinet, C. Bester, M. Brierley, A. Boehme, L. |
author_facet |
Biermann, L. Guinet, C. Bester, M. Brierley, A. Boehme, L. |
author_sort |
Biermann, L. |
title |
An alternative method for correcting fluorescence quenching |
title_short |
An alternative method for correcting fluorescence quenching |
title_full |
An alternative method for correcting fluorescence quenching |
title_fullStr |
An alternative method for correcting fluorescence quenching |
title_full_unstemmed |
An alternative method for correcting fluorescence quenching |
title_sort |
alternative method for correcting fluorescence quenching |
publisher |
HAL CCSD |
publishDate |
2015 |
url |
https://hal.archives-ouvertes.fr/hal-01293313 https://doi.org/10.5194/os-11-83-2015 |
geographic |
Austral Southern Ocean |
geographic_facet |
Austral Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
ISSN: 1812-0784 EISSN: 1812-0792 Ocean Science https://hal.archives-ouvertes.fr/hal-01293313 Ocean Science, 2015, 11 (1), pp.83-91. ⟨10.5194/os-11-83-2015⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.5194/os-11-83-2015 hal-01293313 https://hal.archives-ouvertes.fr/hal-01293313 doi:10.5194/os-11-83-2015 |
op_doi |
https://doi.org/10.5194/os-11-83-2015 |
container_title |
Ocean Science |
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11 |
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1 |
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83 |
op_container_end_page |
91 |
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1766207079506247680 |