Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools.
International audience : The oncosphere stage of Echinococcus multilocularis in red fox stools can lead, after ingestion, to the development of alveolar echinococcosis in the intermediate hosts, commonly small mammals and occasionally humans. Monitoring animal infection and environmental contaminati...
Published in: | Veterinary Parasitology |
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Online Access: | https://hal.science/hal-00943686 https://doi.org/10.1016/j.vetpar.2013.12.023 |
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ftunivnantes:oai:HAL:hal-00943686v1 2023-05-15T16:30:03+02:00 Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. Knapp, Jenny Millon, Laurence Mouzon, Lorane Umhang, Gérald Raoul, Francis Ali, Zeinaba Said Combes, Benoît Comte, Sébastien Gbaguidi-Haore, Houssein Grenouillet, Frédéric Giraudoux, Patrick Laboratoire Chrono-environnement (UMR 6249) (LCE) Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC) Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon) Laboratoire de la rage et pathologie des animaux sauvages (LERPAS) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ) Etablissement Public Interdépartemental Institut Universitaire de France (IUF) Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.) JSPS/CNRS funding 2014 https://hal.science/hal-00943686 https://doi.org/10.1016/j.vetpar.2013.12.023 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2013.12.023 info:eu-repo/semantics/altIdentifier/pmid/24484767 hal-00943686 https://hal.science/hal-00943686 doi:10.1016/j.vetpar.2013.12.023 PRODINRA: 259730 PUBMED: 24484767 WOS: 000333881300006 ISSN: 0304-4017 Veterinary Parasitology https://hal.science/hal-00943686 Veterinary Parasitology, 2014, 201 (1-2), pp.40-47. ⟨10.1016/j.vetpar.2013.12.023⟩ Echinococcus multilocularis SSCT POLYMERASE-CHAIN-REACTION EGGS CARNIVORES FECES Vulpes vulpes Environmental contamination DNA qPCR Fox faeces VULPES-VULPES DIAGNOSIS LIMITS [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2014 ftunivnantes https://doi.org/10.1016/j.vetpar.2013.12.023 2023-02-28T23:49:58Z International audience : The oncosphere stage of Echinococcus multilocularis in red fox stools can lead, after ingestion, to the development of alveolar echinococcosis in the intermediate hosts, commonly small mammals and occasionally humans. Monitoring animal infection and environmental contamination is a key issue in public health surveillance. We developed a quantitative real-time PCR technique (qPCR) to detect and quantify E. multilocularis DNA released in fox faeces. A qPCR technique using a hydrolysis probe targeting part of the mitochondrial gene rrnL was assessed on (i) a reference collection of stools from 57 necropsied foxes simultaneously investigated using the segmental sedimentation and counting technique (SSCT) (29 positive for E. multilocularis worms and 28 negative animals for the parasite); (ii) a collection of 114 fox stools sampled in the field: two sets of 50 samples from contrasted endemic regions in France and 14 from an E. multilocularis-free area (Greenland). Of the negative SSCT controls, 26/28 were qPCR-negative and two were weakly positive. Of the positive SSCT foxes, 25/29 samples were found to be positive by qPCR. Of the field samples, qPCR was positive in 21/50 (42%) and 5/48 (10.4%) stools (2 samples inhibited), originating respectively from high and low endemic areas. In faeces, averages of 0.1pg/μl of DNA in the Jura area and 0.7pg/μl in the Saône-et-Loire area were detected. All qPCR-positive samples were confirmed by sequencing. The qPCR technique developed here allowed us to quantify environmental E. multilocularis contamination by fox faeces by studying the infectious agent directly. No previous study had performed this test in a one-step reaction. Article in Journal/Newspaper Greenland Université de Nantes: HAL-UNIV-NANTES Greenland Jura ENVELOPE(13.501,13.501,68.062,68.062) Veterinary Parasitology 201 1-2 40 47 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Echinococcus multilocularis SSCT POLYMERASE-CHAIN-REACTION EGGS CARNIVORES FECES Vulpes vulpes Environmental contamination DNA qPCR Fox faeces VULPES-VULPES DIAGNOSIS LIMITS [SDV]Life Sciences [q-bio] |
spellingShingle |
Echinococcus multilocularis SSCT POLYMERASE-CHAIN-REACTION EGGS CARNIVORES FECES Vulpes vulpes Environmental contamination DNA qPCR Fox faeces VULPES-VULPES DIAGNOSIS LIMITS [SDV]Life Sciences [q-bio] Knapp, Jenny Millon, Laurence Mouzon, Lorane Umhang, Gérald Raoul, Francis Ali, Zeinaba Said Combes, Benoît Comte, Sébastien Gbaguidi-Haore, Houssein Grenouillet, Frédéric Giraudoux, Patrick Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. |
topic_facet |
Echinococcus multilocularis SSCT POLYMERASE-CHAIN-REACTION EGGS CARNIVORES FECES Vulpes vulpes Environmental contamination DNA qPCR Fox faeces VULPES-VULPES DIAGNOSIS LIMITS [SDV]Life Sciences [q-bio] |
description |
International audience : The oncosphere stage of Echinococcus multilocularis in red fox stools can lead, after ingestion, to the development of alveolar echinococcosis in the intermediate hosts, commonly small mammals and occasionally humans. Monitoring animal infection and environmental contamination is a key issue in public health surveillance. We developed a quantitative real-time PCR technique (qPCR) to detect and quantify E. multilocularis DNA released in fox faeces. A qPCR technique using a hydrolysis probe targeting part of the mitochondrial gene rrnL was assessed on (i) a reference collection of stools from 57 necropsied foxes simultaneously investigated using the segmental sedimentation and counting technique (SSCT) (29 positive for E. multilocularis worms and 28 negative animals for the parasite); (ii) a collection of 114 fox stools sampled in the field: two sets of 50 samples from contrasted endemic regions in France and 14 from an E. multilocularis-free area (Greenland). Of the negative SSCT controls, 26/28 were qPCR-negative and two were weakly positive. Of the positive SSCT foxes, 25/29 samples were found to be positive by qPCR. Of the field samples, qPCR was positive in 21/50 (42%) and 5/48 (10.4%) stools (2 samples inhibited), originating respectively from high and low endemic areas. In faeces, averages of 0.1pg/μl of DNA in the Jura area and 0.7pg/μl in the Saône-et-Loire area were detected. All qPCR-positive samples were confirmed by sequencing. The qPCR technique developed here allowed us to quantify environmental E. multilocularis contamination by fox faeces by studying the infectious agent directly. No previous study had performed this test in a one-step reaction. |
author2 |
Laboratoire Chrono-environnement (UMR 6249) (LCE) Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC) Université Bourgogne Franche-Comté COMUE (UBFC)-Université Bourgogne Franche-Comté COMUE (UBFC) Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon) Laboratoire de la rage et pathologie des animaux sauvages (LERPAS) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Entente de Lutte Interdépartementale contre les Zoonoses (ELIZ) Etablissement Public Interdépartemental Institut Universitaire de France (IUF) Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.) JSPS/CNRS funding |
format |
Article in Journal/Newspaper |
author |
Knapp, Jenny Millon, Laurence Mouzon, Lorane Umhang, Gérald Raoul, Francis Ali, Zeinaba Said Combes, Benoît Comte, Sébastien Gbaguidi-Haore, Houssein Grenouillet, Frédéric Giraudoux, Patrick |
author_facet |
Knapp, Jenny Millon, Laurence Mouzon, Lorane Umhang, Gérald Raoul, Francis Ali, Zeinaba Said Combes, Benoît Comte, Sébastien Gbaguidi-Haore, Houssein Grenouillet, Frédéric Giraudoux, Patrick |
author_sort |
Knapp, Jenny |
title |
Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. |
title_short |
Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. |
title_full |
Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. |
title_fullStr |
Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. |
title_full_unstemmed |
Real time PCR to detect the environmental faecal contamination by Echinococcus multilocularis from red fox stools. |
title_sort |
real time pcr to detect the environmental faecal contamination by echinococcus multilocularis from red fox stools. |
publisher |
HAL CCSD |
publishDate |
2014 |
url |
https://hal.science/hal-00943686 https://doi.org/10.1016/j.vetpar.2013.12.023 |
long_lat |
ENVELOPE(13.501,13.501,68.062,68.062) |
geographic |
Greenland Jura |
geographic_facet |
Greenland Jura |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
ISSN: 0304-4017 Veterinary Parasitology https://hal.science/hal-00943686 Veterinary Parasitology, 2014, 201 (1-2), pp.40-47. ⟨10.1016/j.vetpar.2013.12.023⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2013.12.023 info:eu-repo/semantics/altIdentifier/pmid/24484767 hal-00943686 https://hal.science/hal-00943686 doi:10.1016/j.vetpar.2013.12.023 PRODINRA: 259730 PUBMED: 24484767 WOS: 000333881300006 |
op_doi |
https://doi.org/10.1016/j.vetpar.2013.12.023 |
container_title |
Veterinary Parasitology |
container_volume |
201 |
container_issue |
1-2 |
container_start_page |
40 |
op_container_end_page |
47 |
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1766019766108028928 |