Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas.
International audience In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to foo...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Online Access: | https://hal.univ-brest.fr/hal-00788469 https://doi.org/10.1016/j.cbpb.2012.05.003 |
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ftunivnantes:oai:HAL:hal-00788469v1 2023-05-15T15:57:42+02:00 Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. Huvet, Arnaud Jeffroy, F. Daniel, J. Y. Quéré, C. Le Souchu, P. van Wormhoudt, A. Boudry, Pierre Moal, J. Samain, J. F. Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) 2012-09 https://hal.univ-brest.fr/hal-00788469 https://doi.org/10.1016/j.cbpb.2012.05.003 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpb.2012.05.003 info:eu-repo/semantics/altIdentifier/pmid/22580267 hal-00788469 https://hal.univ-brest.fr/hal-00788469 doi:10.1016/j.cbpb.2012.05.003 PUBMED: 22580267 ISSN: 1096-4959 Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology https://hal.univ-brest.fr/hal-00788469 Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 2012, 163 (1), pp.96-100. ⟨10.1016/j.cbpb.2012.05.003⟩ α-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftunivnantes https://doi.org/10.1016/j.cbpb.2012.05.003 2023-02-08T00:16:16Z International audience In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values and a decrease in total amylase activity. This modulation is the first evidence of observable functioning of AMYB in digestive processes. Amylase B is suggested to display a higher Km than amylase A, offering a means of adapting to high substrate concentrations. The highest starch supplement level (10 mgL(-1)) induced alteration in oyster physiology. The 1 mgL(-1) treatment should be tested as a practical food supplement that could lead to growth benefits for oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 163 1 96 100 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
α-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation [SDE]Environmental Sciences |
spellingShingle |
α-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation [SDE]Environmental Sciences Huvet, Arnaud Jeffroy, F. Daniel, J. Y. Quéré, C. Le Souchu, P. van Wormhoudt, A. Boudry, Pierre Moal, J. Samain, J. F. Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. |
topic_facet |
α-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation [SDE]Environmental Sciences |
description |
International audience In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values and a decrease in total amylase activity. This modulation is the first evidence of observable functioning of AMYB in digestive processes. Amylase B is suggested to display a higher Km than amylase A, offering a means of adapting to high substrate concentrations. The highest starch supplement level (10 mgL(-1)) induced alteration in oyster physiology. The 1 mgL(-1) treatment should be tested as a practical food supplement that could lead to growth benefits for oysters. |
author2 |
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station de Biologie Marine de Concarneau Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE) Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Huvet, Arnaud Jeffroy, F. Daniel, J. Y. Quéré, C. Le Souchu, P. van Wormhoudt, A. Boudry, Pierre Moal, J. Samain, J. F. |
author_facet |
Huvet, Arnaud Jeffroy, F. Daniel, J. Y. Quéré, C. Le Souchu, P. van Wormhoudt, A. Boudry, Pierre Moal, J. Samain, J. F. |
author_sort |
Huvet, Arnaud |
title |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. |
title_short |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. |
title_full |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. |
title_fullStr |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. |
title_full_unstemmed |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas. |
title_sort |
starch supplementation modulates amylase enzymatic properties and amylase b mrna level in the digestive gland of the pacific oyster crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00788469 https://doi.org/10.1016/j.cbpb.2012.05.003 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 1096-4959 Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology https://hal.univ-brest.fr/hal-00788469 Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 2012, 163 (1), pp.96-100. ⟨10.1016/j.cbpb.2012.05.003⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpb.2012.05.003 info:eu-repo/semantics/altIdentifier/pmid/22580267 hal-00788469 https://hal.univ-brest.fr/hal-00788469 doi:10.1016/j.cbpb.2012.05.003 PUBMED: 22580267 |
op_doi |
https://doi.org/10.1016/j.cbpb.2012.05.003 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
163 |
container_issue |
1 |
container_start_page |
96 |
op_container_end_page |
100 |
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