Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.

International audience Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (...

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Published in:Marine Biotechnology
Main Authors: Fleury, Elodie, Huvet, Arnaud
Other Authors: Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL), LITTORAL (LITTORAL), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 2012
Subjects:
Online Access:https://hal.univ-brest.fr/hal-00788265
https://doi.org/10.1007/s10126-011-9403-6
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spelling ftunivnantes:oai:HAL:hal-00788265v1 2023-05-15T15:58:54+02:00 Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality. Fleury, Elodie Huvet, Arnaud Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL) LITTORAL (LITTORAL) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) 2012-04 https://hal.univ-brest.fr/hal-00788265 https://doi.org/10.1007/s10126-011-9403-6 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-011-9403-6 info:eu-repo/semantics/altIdentifier/pmid/21845383 hal-00788265 https://hal.univ-brest.fr/hal-00788265 doi:10.1007/s10126-011-9403-6 PUBMED: 21845383 ISSN: 1436-2228 EISSN: 1436-2236 Marine Biotechnology https://hal.univ-brest.fr/hal-00788265 Marine Biotechnology, 2012, 14 (2), pp.203-17. ⟨10.1007/s10126-011-9403-6⟩ [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftunivnantes https://doi.org/10.1007/s10126-011-9403-6 2023-02-15T00:22:58Z International audience Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (R) or susceptible (S) to summer mortality. Previous genome-wide expression profiling study of R and S oyster gonads highlighted reproduction and antioxidant defense as constitutive pathways that operate differentially between these two lines. Here, we show that signaling in innate immunity also operates differentially between these lines, and we hypothesize that this is at the main determinant of their difference in survival in the field. A reanalysis of our published microarray data using separate ANOVAs at each sampling date revealed a specific "immune" profile at the date preceding the mortality. In addition, we conducted additional microarray profiling of two other tissues, gills, and muscle, and both showed an overrepresentation of immune genes (46%) among those that are differentially expressed between the two lines. Eleven genes were pinpointed to be simultaneously differentially expressed between R and S lines in the three tissues. Among them, ten are related to "Immune Response." For these genes, the kinetics of R mRNA levels between sampling dates appeared different just before the morality peak and suggests that under field conditions, R oysters had the capacity to modulate signaling in innate immunity whereas S oysters did not. This study enhances our understanding of the complex summer mortality syndrome and provides candidates of interest for further functional and genetics studies. Article in Journal/Newspaper Crassostrea gigas Université de Nantes: HAL-UNIV-NANTES Pacific Marine Biotechnology 14 2 203 217
institution Open Polar
collection Université de Nantes: HAL-UNIV-NANTES
op_collection_id ftunivnantes
language English
topic [SDE]Environmental Sciences
spellingShingle [SDE]Environmental Sciences
Fleury, Elodie
Huvet, Arnaud
Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
topic_facet [SDE]Environmental Sciences
description International audience Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (R) or susceptible (S) to summer mortality. Previous genome-wide expression profiling study of R and S oyster gonads highlighted reproduction and antioxidant defense as constitutive pathways that operate differentially between these two lines. Here, we show that signaling in innate immunity also operates differentially between these lines, and we hypothesize that this is at the main determinant of their difference in survival in the field. A reanalysis of our published microarray data using separate ANOVAs at each sampling date revealed a specific "immune" profile at the date preceding the mortality. In addition, we conducted additional microarray profiling of two other tissues, gills, and muscle, and both showed an overrepresentation of immune genes (46%) among those that are differentially expressed between the two lines. Eleven genes were pinpointed to be simultaneously differentially expressed between R and S lines in the three tissues. Among them, ten are related to "Immune Response." For these genes, the kinetics of R mRNA levels between sampling dates appeared different just before the morality peak and suggests that under field conditions, R oysters had the capacity to modulate signaling in innate immunity whereas S oysters did not. This study enhances our understanding of the complex summer mortality syndrome and provides candidates of interest for further functional and genetics studies.
author2 Laboratoire Environnement Ressources Morbihan Pays de Loire (LERMPL)
LITTORAL (LITTORAL)
Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR)
Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM)
Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
format Article in Journal/Newspaper
author Fleury, Elodie
Huvet, Arnaud
author_facet Fleury, Elodie
Huvet, Arnaud
author_sort Fleury, Elodie
title Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
title_short Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
title_full Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
title_fullStr Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
title_full_unstemmed Microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
title_sort microarray analysis highlights immune response of pacific oysters as a determinant of resistance to summer mortality.
publisher HAL CCSD
publishDate 2012
url https://hal.univ-brest.fr/hal-00788265
https://doi.org/10.1007/s10126-011-9403-6
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
genre_facet Crassostrea gigas
op_source ISSN: 1436-2228
EISSN: 1436-2236
Marine Biotechnology
https://hal.univ-brest.fr/hal-00788265
Marine Biotechnology, 2012, 14 (2), pp.203-17. ⟨10.1007/s10126-011-9403-6⟩
op_relation info:eu-repo/semantics/altIdentifier/doi/10.1007/s10126-011-9403-6
info:eu-repo/semantics/altIdentifier/pmid/21845383
hal-00788265
https://hal.univ-brest.fr/hal-00788265
doi:10.1007/s10126-011-9403-6
PUBMED: 21845383
op_doi https://doi.org/10.1007/s10126-011-9403-6
container_title Marine Biotechnology
container_volume 14
container_issue 2
container_start_page 203
op_container_end_page 217
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