Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg).
International audience The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, F...
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Language: | English |
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ftunivnantes:oai:HAL:hal-00770986v1 2023-05-15T15:58:11+02:00 Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). Bado-Nilles, Anne Gagnaire, Béatrice Thomas-Guyon, Hélène Le Floch, Stéphane Renault, Tristan Laboratoire de Ploufragan - Plouzané Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Service de Recherche et d'Expertise sur les Risques environnementaux (IRSN/PRP-ENV/SERIS) Institut de Radioprotection et de Sûreté Nucléaire (IRSN) LIttoral ENvironnement et Sociétés (LIENSs) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre) Cedre Laboratoire de Génétique et Pathologie (LGP) Amélioration génétique, du contrôle des performances et de la santé des mollusques marins (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) 2008-09 https://hal.science/hal-00770986 https://doi.org/10.1016/j.tiv.2008.04.011 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2008.04.011 info:eu-repo/semantics/altIdentifier/pmid/18524534 hal-00770986 https://hal.science/hal-00770986 doi:10.1016/j.tiv.2008.04.011 PUBMED: 18524534 ISSN: 0887-2333 Toxicology in Vitro https://hal.science/hal-00770986 Toxicology in Vitro, 2008, 22 (6), pp.1610-7. ⟨10.1016/j.tiv.2008.04.011⟩ MESH: Crassostrea MESH: Animals MESH: Dose-Response Relationship Drug MESH: Hemocytes MESH: Hemolymph MESH: Oils MESH: Phagocytosis MESH: Polycyclic Hydrocarbons Aromatic MESH: Time Factors MESH: Water Pollutants Chemical [SDV.EE]Life Sciences [q-bio]/Ecology environment info:eu-repo/semantics/article Journal articles 2008 ftunivnantes https://doi.org/10.1016/j.tiv.2008.04.011 2023-03-08T00:04:16Z International audience The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Toxicology in Vitro 22 6 1610 1617 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
MESH: Crassostrea MESH: Animals MESH: Dose-Response Relationship Drug MESH: Hemocytes MESH: Hemolymph MESH: Oils MESH: Phagocytosis MESH: Polycyclic Hydrocarbons Aromatic MESH: Time Factors MESH: Water Pollutants Chemical [SDV.EE]Life Sciences [q-bio]/Ecology environment |
spellingShingle |
MESH: Crassostrea MESH: Animals MESH: Dose-Response Relationship Drug MESH: Hemocytes MESH: Hemolymph MESH: Oils MESH: Phagocytosis MESH: Polycyclic Hydrocarbons Aromatic MESH: Time Factors MESH: Water Pollutants Chemical [SDV.EE]Life Sciences [q-bio]/Ecology environment Bado-Nilles, Anne Gagnaire, Béatrice Thomas-Guyon, Hélène Le Floch, Stéphane Renault, Tristan Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). |
topic_facet |
MESH: Crassostrea MESH: Animals MESH: Dose-Response Relationship Drug MESH: Hemocytes MESH: Hemolymph MESH: Oils MESH: Phagocytosis MESH: Polycyclic Hydrocarbons Aromatic MESH: Time Factors MESH: Water Pollutants Chemical [SDV.EE]Life Sciences [q-bio]/Ecology environment |
description |
International audience The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases. |
author2 |
Laboratoire de Ploufragan - Plouzané Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Service de Recherche et d'Expertise sur les Risques environnementaux (IRSN/PRP-ENV/SERIS) Institut de Radioprotection et de Sûreté Nucléaire (IRSN) LIttoral ENvironnement et Sociétés (LIENSs) La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) Centre de documentation de recherche et d'expérimentations sur les pollutions accidentelles des eaux (Cedre) Cedre Laboratoire de Génétique et Pathologie (LGP) Amélioration génétique, du contrôle des performances et de la santé des mollusques marins (AGSAE) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
format |
Article in Journal/Newspaper |
author |
Bado-Nilles, Anne Gagnaire, Béatrice Thomas-Guyon, Hélène Le Floch, Stéphane Renault, Tristan |
author_facet |
Bado-Nilles, Anne Gagnaire, Béatrice Thomas-Guyon, Hélène Le Floch, Stéphane Renault, Tristan |
author_sort |
Bado-Nilles, Anne |
title |
Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). |
title_short |
Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). |
title_full |
Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). |
title_fullStr |
Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). |
title_full_unstemmed |
Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg). |
title_sort |
effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the pacific oyster, crassostrea gigas (thunberg). |
publisher |
HAL CCSD |
publishDate |
2008 |
url |
https://hal.science/hal-00770986 https://doi.org/10.1016/j.tiv.2008.04.011 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0887-2333 Toxicology in Vitro https://hal.science/hal-00770986 Toxicology in Vitro, 2008, 22 (6), pp.1610-7. ⟨10.1016/j.tiv.2008.04.011⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2008.04.011 info:eu-repo/semantics/altIdentifier/pmid/18524534 hal-00770986 https://hal.science/hal-00770986 doi:10.1016/j.tiv.2008.04.011 PUBMED: 18524534 |
op_doi |
https://doi.org/10.1016/j.tiv.2008.04.011 |
container_title |
Toxicology in Vitro |
container_volume |
22 |
container_issue |
6 |
container_start_page |
1610 |
op_container_end_page |
1617 |
_version_ |
1766393912830722048 |