Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France
International audience Genetic and ecophysiological responses of oyster, Crassostrea gigas, populations to environmental stress were investigated in three highly contaminated French estuaries (the Gironde, Loire and Vilaine) and compared to a control, the Belon estuary. A strong response in both met...
Published in: | Aquatic Toxicology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hal.univ-brest.fr/hal-00660804 https://doi.org/10.1016/j.aquatox.2011.11.014 |
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ftunivnantes:oai:HAL:hal-00660804v1 2023-05-15T15:57:40+02:00 Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France David, Elise Tanguy, Arnaud Riso, Ricardo, Quiniou, Louis Laroche, Jean Moraga, Dario Laboratoire d'Eco-Toxicologie Université de Reims Champagne-Ardenne (URCA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) 2012-03-01 https://hal.univ-brest.fr/hal-00660804 https://doi.org/10.1016/j.aquatox.2011.11.014 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2011.11.014 hal-00660804 https://hal.univ-brest.fr/hal-00660804 doi:10.1016/j.aquatox.2011.11.014 ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00660804 Aquatic Toxicology, 2012, 109, pp.70-79. ⟨10.1016/j.aquatox.2011.11.014⟩ Crassostrea gigas Metallothionein Genetic polymorphism Phenotype-genotype coupling Environmental stress Heavy metals [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2012 ftunivnantes https://doi.org/10.1016/j.aquatox.2011.11.014 2022-12-13T23:43:36Z International audience Genetic and ecophysiological responses of oyster, Crassostrea gigas, populations to environmental stress were investigated in three highly contaminated French estuaries (the Gironde, Loire and Vilaine) and compared to a control, the Belon estuary. A strong response in both metallothionein CgMT4 mRNA expression, as determined by semi-quantitative RT-PCR, and amount of protein, as determined by ELISA tests, was demonstrated in estuarine populations subjected to differential contamination, with an inhibition in the area most highly contaminated with metals. In these same estuarine populations, we found polymorphisms of the metallothionein CgMT4 gene and three other genes (glutamine synthetase - GS, delta-9 desaturase - D9 and phosphoglucomutase - PGM) involved in stress response of C. gigas. We showed that genetic differentiation was observed for MT4 and PGM genes in the Gironde estuary which is highly contaminated with metals. A strong seasonal effect was observed. Phenotype-genotype coupling revealed that one particular MT4 allele and one PGM allele seemed to be associated with metal sensitivity expressed as lower detoxification efficiency and higher metal bioaccumulation. The MT4 gene is a good physiological and genetic marker of stress response and susceptibility. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Université de Nantes: HAL-UNIV-NANTES Pacific Aquatic Toxicology 109 70 79 |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
Crassostrea gigas Metallothionein Genetic polymorphism Phenotype-genotype coupling Environmental stress Heavy metals [SDE]Environmental Sciences |
spellingShingle |
Crassostrea gigas Metallothionein Genetic polymorphism Phenotype-genotype coupling Environmental stress Heavy metals [SDE]Environmental Sciences David, Elise Tanguy, Arnaud Riso, Ricardo, Quiniou, Louis Laroche, Jean Moraga, Dario Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France |
topic_facet |
Crassostrea gigas Metallothionein Genetic polymorphism Phenotype-genotype coupling Environmental stress Heavy metals [SDE]Environmental Sciences |
description |
International audience Genetic and ecophysiological responses of oyster, Crassostrea gigas, populations to environmental stress were investigated in three highly contaminated French estuaries (the Gironde, Loire and Vilaine) and compared to a control, the Belon estuary. A strong response in both metallothionein CgMT4 mRNA expression, as determined by semi-quantitative RT-PCR, and amount of protein, as determined by ELISA tests, was demonstrated in estuarine populations subjected to differential contamination, with an inhibition in the area most highly contaminated with metals. In these same estuarine populations, we found polymorphisms of the metallothionein CgMT4 gene and three other genes (glutamine synthetase - GS, delta-9 desaturase - D9 and phosphoglucomutase - PGM) involved in stress response of C. gigas. We showed that genetic differentiation was observed for MT4 and PGM genes in the Gironde estuary which is highly contaminated with metals. A strong seasonal effect was observed. Phenotype-genotype coupling revealed that one particular MT4 allele and one PGM allele seemed to be associated with metal sensitivity expressed as lower detoxification efficiency and higher metal bioaccumulation. The MT4 gene is a good physiological and genetic marker of stress response and susceptibility. |
author2 |
Laboratoire d'Eco-Toxicologie Université de Reims Champagne-Ardenne (URCA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Adaptation et diversité en milieu marin (AD2M) Station biologique de Roscoff Roscoff (SBR) Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Station biologique de Roscoff (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
David, Elise Tanguy, Arnaud Riso, Ricardo, Quiniou, Louis Laroche, Jean Moraga, Dario |
author_facet |
David, Elise Tanguy, Arnaud Riso, Ricardo, Quiniou, Louis Laroche, Jean Moraga, Dario |
author_sort |
David, Elise |
title |
Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France |
title_short |
Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France |
title_full |
Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France |
title_fullStr |
Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France |
title_full_unstemmed |
Responses of Pacific oyster Crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the Atlantic coast of France |
title_sort |
responses of pacific oyster crassostrea gigas populations to abiotic stress in environmentally contrasted estuaries along the atlantic coast of france |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.univ-brest.fr/hal-00660804 https://doi.org/10.1016/j.aquatox.2011.11.014 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0166-445X Aquatic Toxicology https://hal.univ-brest.fr/hal-00660804 Aquatic Toxicology, 2012, 109, pp.70-79. ⟨10.1016/j.aquatox.2011.11.014⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquatox.2011.11.014 hal-00660804 https://hal.univ-brest.fr/hal-00660804 doi:10.1016/j.aquatox.2011.11.014 |
op_doi |
https://doi.org/10.1016/j.aquatox.2011.11.014 |
container_title |
Aquatic Toxicology |
container_volume |
109 |
container_start_page |
70 |
op_container_end_page |
79 |
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1766393337725583360 |