Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean
International audience We determined the δ15N and δ13C values of individual amino acids (AAs) isolated from chick blood of four penguin species that forage in different oceanic regions (from the subtropics of the Indian Ocean to Antarctica) to test if (1) the δ15N values of phenylalanine (δ15Nphe) r...
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ftunivnantes:oai:HAL:hal-00452366v1 2023-05-15T13:53:36+02:00 Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean Lorrain, Anne Graham, Brittany Ménard, Frédéric Popp, Brian Bouillon, S. van Breugel, Peter Cherel, Yves Thons tropicaux et écosystèmes pélagiques : taxies, interactions et stratégies d'exploitation (THETIS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) University of Hawai‘i Mānoa (UHM) UMR 212 EME "écosystèmes marins exploités" (EME) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM) Vrije Universiteit Brussel (VUB) Netherlands Institute of Ecology (NIOO) Netherlands Institute of Ecology Centre d'Études Biologiques de Chizé (CEBC) Centre National de la Recherche Scientifique (CNRS) 2009-09-28 https://hal.science/hal-00452366 https://hal.science/hal-00452366/document https://hal.science/hal-00452366/file/LorrainMEPSrev.pdf en eng HAL CCSD Inter Research hal-00452366 https://hal.science/hal-00452366 https://hal.science/hal-00452366/document https://hal.science/hal-00452366/file/LorrainMEPSrev.pdf IRD: fdi:010048327 info:eu-repo/semantics/OpenAccess ISSN: 0171-8630 EISSN: 1616-1599 Marine Ecology Progress Series https://hal.science/hal-00452366 Marine Ecology Progress Series, 2009, 391, pp.293-306 [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2009 ftunivnantes 2023-02-08T01:02:24Z International audience We determined the δ15N and δ13C values of individual amino acids (AAs) isolated from chick blood of four penguin species that forage in different oceanic regions (from the subtropics of the Indian Ocean to Antarctica) to test if (1) the δ15N values of phenylalanine (δ15Nphe) revealed different foraging areas among the species, (2) the difference between glutamic acid and phenylalanine δ15N values (Δδ15Nglu-phe) accurately predicted trophic levels (TL), and (3) the δ13C value of AAs could resolve species foraging locations, as bulk δ13C values did. The δ13C values of all AAs decreased with latitude, were positively correlated with bulk δ13C data, and therefore, tracked the isotopic baseline. However, we were not able to discern additional ecological information from these δ13C values. In contrast, the δ15N analysis of individual AAs in blood distinguished the isotopic value of the nitrogen at the base of the food web from the trophic level of the consumer, providing new insight for the study of the trophic ecology of seabirds. The difference in the bulk δ15N values of northern and southern rockhopper penguins was due to both a difference in their foraging location (≠δ15Nphe) and their trophic levels (≠Δδ15Nglu-phe). The δ15Nphe values of king and Adélie penguins were higher than those from rockhoppers and we hypothesize that this difference reflects foraging on mesopelagic prey and in the highly productive Antarctic shelf waters respectively. The Δδ15Nglu-phe accurately reflected relative penguin's TL but further work is required to determine the trophic enrichment factors for compound-specific isotope analysis. Article in Journal/Newspaper Antarc* Antarctic Antarctica Southern Ocean Université de Nantes: HAL-UNIV-NANTES Antarctic Southern Ocean Indian |
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Open Polar |
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Université de Nantes: HAL-UNIV-NANTES |
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ftunivnantes |
language |
English |
topic |
[SDE]Environmental Sciences |
spellingShingle |
[SDE]Environmental Sciences Lorrain, Anne Graham, Brittany Ménard, Frédéric Popp, Brian Bouillon, S. van Breugel, Peter Cherel, Yves Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean |
topic_facet |
[SDE]Environmental Sciences |
description |
International audience We determined the δ15N and δ13C values of individual amino acids (AAs) isolated from chick blood of four penguin species that forage in different oceanic regions (from the subtropics of the Indian Ocean to Antarctica) to test if (1) the δ15N values of phenylalanine (δ15Nphe) revealed different foraging areas among the species, (2) the difference between glutamic acid and phenylalanine δ15N values (Δδ15Nglu-phe) accurately predicted trophic levels (TL), and (3) the δ13C value of AAs could resolve species foraging locations, as bulk δ13C values did. The δ13C values of all AAs decreased with latitude, were positively correlated with bulk δ13C data, and therefore, tracked the isotopic baseline. However, we were not able to discern additional ecological information from these δ13C values. In contrast, the δ15N analysis of individual AAs in blood distinguished the isotopic value of the nitrogen at the base of the food web from the trophic level of the consumer, providing new insight for the study of the trophic ecology of seabirds. The difference in the bulk δ15N values of northern and southern rockhopper penguins was due to both a difference in their foraging location (≠δ15Nphe) and their trophic levels (≠Δδ15Nglu-phe). The δ15Nphe values of king and Adélie penguins were higher than those from rockhoppers and we hypothesize that this difference reflects foraging on mesopelagic prey and in the highly productive Antarctic shelf waters respectively. The Δδ15Nglu-phe accurately reflected relative penguin's TL but further work is required to determine the trophic enrichment factors for compound-specific isotope analysis. |
author2 |
Thons tropicaux et écosystèmes pélagiques : taxies, interactions et stratégies d'exploitation (THETIS) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) University of Hawai‘i Mānoa (UHM) UMR 212 EME "écosystèmes marins exploités" (EME) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM) Vrije Universiteit Brussel (VUB) Netherlands Institute of Ecology (NIOO) Netherlands Institute of Ecology Centre d'Études Biologiques de Chizé (CEBC) Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Lorrain, Anne Graham, Brittany Ménard, Frédéric Popp, Brian Bouillon, S. van Breugel, Peter Cherel, Yves |
author_facet |
Lorrain, Anne Graham, Brittany Ménard, Frédéric Popp, Brian Bouillon, S. van Breugel, Peter Cherel, Yves |
author_sort |
Lorrain, Anne |
title |
Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean |
title_short |
Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean |
title_full |
Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean |
title_fullStr |
Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean |
title_full_unstemmed |
Nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the Southern Ocean |
title_sort |
nitrogen and carbon isotope values of individual amino acids: a tool to study foraging ecology of penguins in the southern ocean |
publisher |
HAL CCSD |
publishDate |
2009 |
url |
https://hal.science/hal-00452366 https://hal.science/hal-00452366/document https://hal.science/hal-00452366/file/LorrainMEPSrev.pdf |
geographic |
Antarctic Southern Ocean Indian |
geographic_facet |
Antarctic Southern Ocean Indian |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_source |
ISSN: 0171-8630 EISSN: 1616-1599 Marine Ecology Progress Series https://hal.science/hal-00452366 Marine Ecology Progress Series, 2009, 391, pp.293-306 |
op_relation |
hal-00452366 https://hal.science/hal-00452366 https://hal.science/hal-00452366/document https://hal.science/hal-00452366/file/LorrainMEPSrev.pdf IRD: fdi:010048327 |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766258812075900928 |