Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.).
European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us t...
Published in: | General and Comparative Endocrinology |
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Online Access: | https://hal.science/hal-00150502 https://doi.org/10.1016/j.ygcen.2007.01.009 |
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ftunivnantes:oai:HAL:hal-00150502v1 2023-05-15T13:28:07+02:00 Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). Sébert, Marie-Emilie Amérand, Aline Vettier, Aurélie Weltzien, Finn-Arne Pasqualini, Catherine Sébert, Philippe Dufour, Sylvie Biologie des organismes marins et écosystèmes (BOME) Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Unité Haute Pression et Métabolisme, UPCI-EA3879 Université de Brest (UBO) Développement, évolution et plasticité du système nerveux (DEPSN) Centre National de la Recherche Scientifique (CNRS) Institut de Neurobiologie Alfred Fessard (INAF) 2007 https://hal.science/hal-00150502 https://doi.org/10.1016/j.ygcen.2007.01.009 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2007.01.009 info:eu-repo/semantics/altIdentifier/pmid/17324430 hal-00150502 https://hal.science/hal-00150502 doi:10.1016/j.ygcen.2007.01.009 PUBMED: 17324430 ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.science/hal-00150502 General and Comparative Endocrinology, 2007, 153 (1-3), pp.289-298. ⟨10.1016/j.ygcen.2007.01.009⟩ [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] info:eu-repo/semantics/article Journal articles 2007 ftunivnantes https://doi.org/10.1016/j.ygcen.2007.01.009 2023-02-08T09:37:45Z European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that high hydrostatic pressure (HP) influences the induction of eel reproduction. We subjected female and male silver eels to 101ATA for 3 and 7 weeks, respectively, in a hyperbaric chamber equipped with a freshwater recirculation system. In comparison with control eels kept at 1 ATA, HP effects were tested against the messenger RNA levels of pituitary gonadotropins (LHbeta, FSHbeta) using quantitative real-time RT-PCR. The effects of HP on gonadal activity were estimated by measuring gonadosomatic index, oocyte diameter and plasma levels of vitellogenin (Vtg) and sex steroids (E(2), 11-KT). At the pituitary level, LHbeta expression tended to increase while FSHbeta expression decreased in both sex, leading to an increase in the LHbeta/FSHbeta ratio. This suggests a differential effect of HP on the expression of the two gonadotropins. In females submitted to HP, we observed a significant increase in oocyte diameter and plasma levels of 11-KT and E(2). A similar trend was observed for 11-KT plasma levels in males. In females, Vtg plasma levels also significantly increased, reflecting the stimulatory effect of sex steroids on hepatic vitellogenesis. Our results suggest that HP plays a specific and positive role in eel reproduction but additional environmental and internal factors are necessary to ensure complete sexual maturation. Article in Journal/Newspaper Anguilla anguilla Université de Nantes: HAL-UNIV-NANTES General and Comparative Endocrinology 153 1-3 289 298 |
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Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
English |
topic |
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] |
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[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Sébert, Marie-Emilie Amérand, Aline Vettier, Aurélie Weltzien, Finn-Arne Pasqualini, Catherine Sébert, Philippe Dufour, Sylvie Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). |
topic_facet |
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] |
description |
European silver eels are thought to undergo sexual maturation during their oceanic reproductive migration from the European continent to their spawning area in the Sargasso Sea. Tracking data and various anatomical and physiological features suggest that silver eels migrate in deep sea, leading us to hypothesise that high hydrostatic pressure (HP) influences the induction of eel reproduction. We subjected female and male silver eels to 101ATA for 3 and 7 weeks, respectively, in a hyperbaric chamber equipped with a freshwater recirculation system. In comparison with control eels kept at 1 ATA, HP effects were tested against the messenger RNA levels of pituitary gonadotropins (LHbeta, FSHbeta) using quantitative real-time RT-PCR. The effects of HP on gonadal activity were estimated by measuring gonadosomatic index, oocyte diameter and plasma levels of vitellogenin (Vtg) and sex steroids (E(2), 11-KT). At the pituitary level, LHbeta expression tended to increase while FSHbeta expression decreased in both sex, leading to an increase in the LHbeta/FSHbeta ratio. This suggests a differential effect of HP on the expression of the two gonadotropins. In females submitted to HP, we observed a significant increase in oocyte diameter and plasma levels of 11-KT and E(2). A similar trend was observed for 11-KT plasma levels in males. In females, Vtg plasma levels also significantly increased, reflecting the stimulatory effect of sex steroids on hepatic vitellogenesis. Our results suggest that HP plays a specific and positive role in eel reproduction but additional environmental and internal factors are necessary to ensure complete sexual maturation. |
author2 |
Biologie des organismes marins et écosystèmes (BOME) Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) Unité Haute Pression et Métabolisme, UPCI-EA3879 Université de Brest (UBO) Développement, évolution et plasticité du système nerveux (DEPSN) Centre National de la Recherche Scientifique (CNRS) Institut de Neurobiologie Alfred Fessard (INAF) |
format |
Article in Journal/Newspaper |
author |
Sébert, Marie-Emilie Amérand, Aline Vettier, Aurélie Weltzien, Finn-Arne Pasqualini, Catherine Sébert, Philippe Dufour, Sylvie |
author_facet |
Sébert, Marie-Emilie Amérand, Aline Vettier, Aurélie Weltzien, Finn-Arne Pasqualini, Catherine Sébert, Philippe Dufour, Sylvie |
author_sort |
Sébert, Marie-Emilie |
title |
Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). |
title_short |
Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). |
title_full |
Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). |
title_fullStr |
Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). |
title_full_unstemmed |
Effects of high hydrostatic pressure on the pituitary-gonad axis in the European eel, Anguilla anguilla (L.). |
title_sort |
effects of high hydrostatic pressure on the pituitary-gonad axis in the european eel, anguilla anguilla (l.). |
publisher |
HAL CCSD |
publishDate |
2007 |
url |
https://hal.science/hal-00150502 https://doi.org/10.1016/j.ygcen.2007.01.009 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
ISSN: 0016-6480 EISSN: 1095-6840 General and Comparative Endocrinology https://hal.science/hal-00150502 General and Comparative Endocrinology, 2007, 153 (1-3), pp.289-298. ⟨10.1016/j.ygcen.2007.01.009⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2007.01.009 info:eu-repo/semantics/altIdentifier/pmid/17324430 hal-00150502 https://hal.science/hal-00150502 doi:10.1016/j.ygcen.2007.01.009 PUBMED: 17324430 |
op_doi |
https://doi.org/10.1016/j.ygcen.2007.01.009 |
container_title |
General and Comparative Endocrinology |
container_volume |
153 |
container_issue |
1-3 |
container_start_page |
289 |
op_container_end_page |
298 |
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1766402277237587968 |