DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas
In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechani...
Published in: | Marine Biotechnology |
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2013
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Online Access: | https://researchportal.unamur.be/en/publications/66e5f0e2-d0e0-4112-8054-9f260e76ed29 https://doi.org/10.1007/s10126-013-9523-2 http://www.scopus.com/inward/record.url?scp=84885948837&partnerID=8YFLogxK |
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ftunivnamurcrisp:oai:pure.unamur.be:publications/66e5f0e2-d0e0-4112-8054-9f260e76ed29 2024-09-15T18:03:16+00:00 DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas Riviere, Guillaume Wu, Guan Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal 2013-12 https://researchportal.unamur.be/en/publications/66e5f0e2-d0e0-4112-8054-9f260e76ed29 https://doi.org/10.1007/s10126-013-9523-2 http://www.scopus.com/inward/record.url?scp=84885948837&partnerID=8YFLogxK eng eng https://researchportal.unamur.be/en/publications/66e5f0e2-d0e0-4112-8054-9f260e76ed29 info:eu-repo/semantics/closedAccess Riviere , G , Wu , G C , Fellous , A , Goux , D , Sourdaine , P & Favrel , P 2013 , ' DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas ' , Marine Biotechnology , vol. 15 , no. 6 , pp. 739-753 . https://doi.org/10.1007/s10126-013-9523-2 Development Epigenetics Evolution Homeobox Methylation article 2013 ftunivnamurcrisp https://doi.org/10.1007/s10126-013-9523-2 2024-07-01T14:03:16Z In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechanisms remain poorly understood in more distant organisms such as Lophotrochozoa. Thus, despite DNA in the oyster genome being methylated, the role of DNA methylation in development is unknown. To clarify this point, oyster genomic DNA was examined during early embryogenesis and found differentially methylated. Reverse transcriptase quantitative polymerase chain reaction indicated stage-specific levels of transcripts encoding DNA-methyltransferase (DNMT) and methyl-binding domain proteins. In addition, as highlighted by electronic microscopy and immunohistochemistry, the DNMT inhibitor 5-aza-cytidine induced alterations in the quantity and the localisation of methylated DNA and severe dose-dependent development alterations and was lethal after zygotic genome reinitiation. Furthermore, methyl-DNA-immunoprecipitation-quantitative polymerase chain reaction revealed that the transcription level of most of the homeobox gene orthologues examined, but not of the other early genes investigated, was inversely correlated with their specific DNA methylation. Altogether, our results demonstrate that DNA methylation influences gene expression in Crassostrea gigas and is critical for oyster development, possibly by specifically controlling the transcription level of homeobox orthologues. These findings provide evidence for the importance of epigenetic regulation of development in Lophotrochozoans and bring new insights into the early life of C. gigas, one of the most important aquaculture resources worldwide. Article in Journal/Newspaper Crassostrea gigas Research Portal - University of Namur Marine Biotechnology 15 6 739 753 |
institution |
Open Polar |
collection |
Research Portal - University of Namur |
op_collection_id |
ftunivnamurcrisp |
language |
English |
topic |
Development Epigenetics Evolution Homeobox Methylation |
spellingShingle |
Development Epigenetics Evolution Homeobox Methylation Riviere, Guillaume Wu, Guan Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
topic_facet |
Development Epigenetics Evolution Homeobox Methylation |
description |
In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechanisms remain poorly understood in more distant organisms such as Lophotrochozoa. Thus, despite DNA in the oyster genome being methylated, the role of DNA methylation in development is unknown. To clarify this point, oyster genomic DNA was examined during early embryogenesis and found differentially methylated. Reverse transcriptase quantitative polymerase chain reaction indicated stage-specific levels of transcripts encoding DNA-methyltransferase (DNMT) and methyl-binding domain proteins. In addition, as highlighted by electronic microscopy and immunohistochemistry, the DNMT inhibitor 5-aza-cytidine induced alterations in the quantity and the localisation of methylated DNA and severe dose-dependent development alterations and was lethal after zygotic genome reinitiation. Furthermore, methyl-DNA-immunoprecipitation-quantitative polymerase chain reaction revealed that the transcription level of most of the homeobox gene orthologues examined, but not of the other early genes investigated, was inversely correlated with their specific DNA methylation. Altogether, our results demonstrate that DNA methylation influences gene expression in Crassostrea gigas and is critical for oyster development, possibly by specifically controlling the transcription level of homeobox orthologues. These findings provide evidence for the importance of epigenetic regulation of development in Lophotrochozoans and bring new insights into the early life of C. gigas, one of the most important aquaculture resources worldwide. |
format |
Article in Journal/Newspaper |
author |
Riviere, Guillaume Wu, Guan Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal |
author_facet |
Riviere, Guillaume Wu, Guan Chung Fellous, Alexandre Goux, Didier Sourdaine, Pascal Favrel, Pascal |
author_sort |
Riviere, Guillaume |
title |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_short |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_full |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_fullStr |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_full_unstemmed |
DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas |
title_sort |
dna methylation is crucial for the early development in the oyster c. gigas |
publishDate |
2013 |
url |
https://researchportal.unamur.be/en/publications/66e5f0e2-d0e0-4112-8054-9f260e76ed29 https://doi.org/10.1007/s10126-013-9523-2 http://www.scopus.com/inward/record.url?scp=84885948837&partnerID=8YFLogxK |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Riviere , G , Wu , G C , Fellous , A , Goux , D , Sourdaine , P & Favrel , P 2013 , ' DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas ' , Marine Biotechnology , vol. 15 , no. 6 , pp. 739-753 . https://doi.org/10.1007/s10126-013-9523-2 |
op_relation |
https://researchportal.unamur.be/en/publications/66e5f0e2-d0e0-4112-8054-9f260e76ed29 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s10126-013-9523-2 |
container_title |
Marine Biotechnology |
container_volume |
15 |
container_issue |
6 |
container_start_page |
739 |
op_container_end_page |
753 |
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1810440774668517376 |