Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd
Metallothioneins (MT) were localized by immunochemistry in different organs and cell compartments of turbot exposed to sublethal concentrations (100 ppb) of Cd for 7 days. The polyclonal rabbit anti-cod MT antibody (NIVA, Norway) applied herein exhibited positive cross-reactivity with turbot MTs. Im...
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Murcia : F. Hernández
2007
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ftunivmurdigi:oai:digitum.um.es:10201/27609 2023-05-15T18:15:51+02:00 Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd Alvarado, N.E. Cancio, I. Hylland, K. Marigómez, I. Soto, M. 2007 application/pdf http://hdl.handle.net/10201/27609 eng eng Murcia : F. Hernández Histology and histopathology 0213-3911 http://hdl.handle.net/10201/27609 info:eu-repo/semantics/openAccess Turbot Hepatocyte 576 - Biología celular y subcelular. Citología info:eu-repo/semantics/article 2007 ftunivmurdigi 2017-08-05T14:37:22Z Metallothioneins (MT) were localized by immunochemistry in different organs and cell compartments of turbot exposed to sublethal concentrations (100 ppb) of Cd for 7 days. The polyclonal rabbit anti-cod MT antibody (NIVA, Norway) applied herein exhibited positive cross-reactivity with turbot MTs. Immunoreactive MTs were localized in the branchial epithelium, in the liver and in the kidney of turbot. In Cd exposed fishes MTs were demonstrated mainly in branchial chloride cells (CC) and to a lesser extend in the area where progenitor cells are located and in the cells of the respiratory epithelium (secondary lamellae). A higher staining intensity for MTs was observed in CC of the interlamelar space of the main branchial epithelium in comparison with control CC. MT-staining was also observed in the chondroblasts of the cartilage and in the erythrocytes within blood vessels both in control and Cd-exposed specimens. MT immunoreaction was high in the liver hepatocytes and weak in the epithelium of the proximal portion of the kidney in exposed turbot. The tegument, spleen and muscle were devoid of any immunolabelling in both treatments. Ultrastructural studies at the transmission electron microscope revealed that Cd-induced MTs were mainly located in the cytoplasm of gill CC, the lysosomes and the cytoplasm of hepatocytes and in the basal labyrinth of kidney proximal nephrocytes. The differential localization/induction of MTs in different cell types described hereby suggests that the quantification of the specific expression of MT may be used in biomonitoring programs as a biomarker of Cd exposure in aquatic environments. Article in Journal/Newspaper Scophthalmus maximus Turbot DIGITUM - Depósito Digital Institucional de la Universidad de Murcia Labyrinth ENVELOPE(160.833,160.833,-77.550,-77.550) Niva ENVELOPE(23.913,23.913,66.136,66.136) Norway |
institution |
Open Polar |
collection |
DIGITUM - Depósito Digital Institucional de la Universidad de Murcia |
op_collection_id |
ftunivmurdigi |
language |
English |
topic |
Turbot Hepatocyte 576 - Biología celular y subcelular. Citología |
spellingShingle |
Turbot Hepatocyte 576 - Biología celular y subcelular. Citología Alvarado, N.E. Cancio, I. Hylland, K. Marigómez, I. Soto, M. Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd |
topic_facet |
Turbot Hepatocyte 576 - Biología celular y subcelular. Citología |
description |
Metallothioneins (MT) were localized by immunochemistry in different organs and cell compartments of turbot exposed to sublethal concentrations (100 ppb) of Cd for 7 days. The polyclonal rabbit anti-cod MT antibody (NIVA, Norway) applied herein exhibited positive cross-reactivity with turbot MTs. Immunoreactive MTs were localized in the branchial epithelium, in the liver and in the kidney of turbot. In Cd exposed fishes MTs were demonstrated mainly in branchial chloride cells (CC) and to a lesser extend in the area where progenitor cells are located and in the cells of the respiratory epithelium (secondary lamellae). A higher staining intensity for MTs was observed in CC of the interlamelar space of the main branchial epithelium in comparison with control CC. MT-staining was also observed in the chondroblasts of the cartilage and in the erythrocytes within blood vessels both in control and Cd-exposed specimens. MT immunoreaction was high in the liver hepatocytes and weak in the epithelium of the proximal portion of the kidney in exposed turbot. The tegument, spleen and muscle were devoid of any immunolabelling in both treatments. Ultrastructural studies at the transmission electron microscope revealed that Cd-induced MTs were mainly located in the cytoplasm of gill CC, the lysosomes and the cytoplasm of hepatocytes and in the basal labyrinth of kidney proximal nephrocytes. The differential localization/induction of MTs in different cell types described hereby suggests that the quantification of the specific expression of MT may be used in biomonitoring programs as a biomarker of Cd exposure in aquatic environments. |
format |
Article in Journal/Newspaper |
author |
Alvarado, N.E. Cancio, I. Hylland, K. Marigómez, I. Soto, M. |
author_facet |
Alvarado, N.E. Cancio, I. Hylland, K. Marigómez, I. Soto, M. |
author_sort |
Alvarado, N.E. |
title |
Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd |
title_short |
Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd |
title_full |
Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd |
title_fullStr |
Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd |
title_full_unstemmed |
Immunolocalization of metallothioneins in different tissues of turbot (Scophthalmus maximus) exposed to Cd |
title_sort |
immunolocalization of metallothioneins in different tissues of turbot (scophthalmus maximus) exposed to cd |
publisher |
Murcia : F. Hernández |
publishDate |
2007 |
url |
http://hdl.handle.net/10201/27609 |
long_lat |
ENVELOPE(160.833,160.833,-77.550,-77.550) ENVELOPE(23.913,23.913,66.136,66.136) |
geographic |
Labyrinth Niva Norway |
geographic_facet |
Labyrinth Niva Norway |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Histology and histopathology 0213-3911 http://hdl.handle.net/10201/27609 |
op_rights |
info:eu-repo/semantics/openAccess |
_version_ |
1766189086964449280 |