Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle
The presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). Cryostat sections of epaxial, hypaxial and adductor mandibulae (A...
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Histology and Histopathology
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ftunivmurciaojs:oai:revistas.um.es:article/129581 2023-05-15T13:27:50+02:00 Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle Domeneghini, Cinzia Radaelli, G. Arrighi, S. Mascarello, F. Veggetti, A. 2011-05-19 application/pdf https://revistas.um.es/hh/article/view/129581 eng eng Histology and Histopathology https://revistas.um.es/hh/article/view/129581/120651 https://revistas.um.es/hh/article/view/129581 Histology and Histopathology; Vol. 13 No. 4 (1998); 939-947 Histology and Histopathology; Vol. 13 Núm. 4 (1998); 939-947 1699-5848 0213-3911 teleosts skeletal muscle neuropeptides NOS info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2011 ftunivmurciaojs 2022-04-18T05:55:55Z The presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). Cryostat sections of epaxial, hypaxial and adductor mandibulae (AM) muscles of Sparus aurata and Anguilla anguilla were stained histochemically for reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase. Other sections were used for indirect immunohistochemistry (streptavidin-biotin and rhodamine immunofluorescence methods), employing antibodies specific for putative excitatory or inhibitory peptides, including CGRP, substance P, met-enkephalin, bombesin, and VIP. In addition, ultrastructural observations were performed in order to describe the morphology of the motor endplates. A strong immunoreactivity for CGRP and substance P was found in many nerve terminals. Met-enkephalin, bombesin and VIP immunoreactivities were less frequently observed. No immunoreactivity was observed to CCK, NPY or 5-HT. NADPH-diaphorase was identified in nerve fibres of the AM complex only of A. anguilla. Electron microscopy observations evidenced more than one type of synaptic vesicle in motor endplates. Some differences in putative neuromodulator distributions were observed in the two species and muscle complexes, which may be related to the different taxonomical position as well as the different pattern of innervation of white muscle fibres. Article in Journal/Newspaper Anguilla anguilla Revistas Científicas de la Universidad de Murcia |
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Open Polar |
collection |
Revistas Científicas de la Universidad de Murcia |
op_collection_id |
ftunivmurciaojs |
language |
English |
topic |
teleosts skeletal muscle neuropeptides NOS |
spellingShingle |
teleosts skeletal muscle neuropeptides NOS Domeneghini, Cinzia Radaelli, G. Arrighi, S. Mascarello, F. Veggetti, A. Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
topic_facet |
teleosts skeletal muscle neuropeptides NOS |
description |
The presence of putative neuromodulators in the nerve fibres was investigated in white skeletal muscle of two teleost fish not taxonomically correlated and showing different patterns of innervation (multiple versus focal innervation). Cryostat sections of epaxial, hypaxial and adductor mandibulae (AM) muscles of Sparus aurata and Anguilla anguilla were stained histochemically for reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase. Other sections were used for indirect immunohistochemistry (streptavidin-biotin and rhodamine immunofluorescence methods), employing antibodies specific for putative excitatory or inhibitory peptides, including CGRP, substance P, met-enkephalin, bombesin, and VIP. In addition, ultrastructural observations were performed in order to describe the morphology of the motor endplates. A strong immunoreactivity for CGRP and substance P was found in many nerve terminals. Met-enkephalin, bombesin and VIP immunoreactivities were less frequently observed. No immunoreactivity was observed to CCK, NPY or 5-HT. NADPH-diaphorase was identified in nerve fibres of the AM complex only of A. anguilla. Electron microscopy observations evidenced more than one type of synaptic vesicle in motor endplates. Some differences in putative neuromodulator distributions were observed in the two species and muscle complexes, which may be related to the different taxonomical position as well as the different pattern of innervation of white muscle fibres. |
format |
Article in Journal/Newspaper |
author |
Domeneghini, Cinzia Radaelli, G. Arrighi, S. Mascarello, F. Veggetti, A. |
author_facet |
Domeneghini, Cinzia Radaelli, G. Arrighi, S. Mascarello, F. Veggetti, A. |
author_sort |
Domeneghini, Cinzia |
title |
Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
title_short |
Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
title_full |
Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
title_fullStr |
Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
title_full_unstemmed |
Different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
title_sort |
different putative neuromodulators are present in the nerves which distribute to the teleost skeletal muscle |
publisher |
Histology and Histopathology |
publishDate |
2011 |
url |
https://revistas.um.es/hh/article/view/129581 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Histology and Histopathology; Vol. 13 No. 4 (1998); 939-947 Histology and Histopathology; Vol. 13 Núm. 4 (1998); 939-947 1699-5848 0213-3911 |
op_relation |
https://revistas.um.es/hh/article/view/129581/120651 https://revistas.um.es/hh/article/view/129581 |
_version_ |
1766400651895504896 |