Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations
Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon recep...
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Online Access: | http://hdl.handle.net/11380/310064 https://doi.org/10.1530/REP-06-0195 |
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ftunivmodena:oai:iris.unimore.it:11380/310064 2024-02-11T10:04:25+01:00 Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations M. Long A. Stronati D. Bizzaro T. Kruger P. S. Hjelmborg M. Spano A. Giwercman G. Toft J. P. Bonde E. C. Bonefeld Jorgensen MANICARDI, Gian Carlo M., Long A., Stronati D., Bizzaro T., Kruger Manicardi, Gian Carlo P. S., Hjelmborg M., Spano A., Giwercman G., Toft J. P., Bonde E. C., Bonefeld Jorgensen 2007 STAMPA http://hdl.handle.net/11380/310064 https://doi.org/10.1530/REP-06-0195 eng eng info:eu-repo/semantics/altIdentifier/pmid/17307920 info:eu-repo/semantics/altIdentifier/wos/WOS:000244680500018 volume:133 issue:2 firstpage:517 lastpage:530 journal:REPRODUCTION http://hdl.handle.net/11380/310064 doi:10.1530/REP-06-0195 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33847675040 POLYCHLORINATED-BIPHENYLS-PCBS EJACULATED-HUMAN-SPERMATOZOA CURRENTLY-USED-PESTICIDES CHROMATIN-STRUCTURE-ASSAY MESSENGER-RNA-LEVELS CELL-APOPTOSIS IN-VITRO AROMATIC-HYDROCARBONS ESTROGENIC-EXPOSURE OXIDATIVE-STRESS info:eu-repo/semantics/article 2007 ftunivmodena https://doi.org/10.1530/REP-06-0195 2024-01-23T23:18:17Z Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population. Article in Journal/Newspaper Greenland inuit inuits Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) Greenland Reproduction 133 2 517 530 |
institution |
Open Polar |
collection |
Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
op_collection_id |
ftunivmodena |
language |
English |
topic |
POLYCHLORINATED-BIPHENYLS-PCBS EJACULATED-HUMAN-SPERMATOZOA CURRENTLY-USED-PESTICIDES CHROMATIN-STRUCTURE-ASSAY MESSENGER-RNA-LEVELS CELL-APOPTOSIS IN-VITRO AROMATIC-HYDROCARBONS ESTROGENIC-EXPOSURE OXIDATIVE-STRESS |
spellingShingle |
POLYCHLORINATED-BIPHENYLS-PCBS EJACULATED-HUMAN-SPERMATOZOA CURRENTLY-USED-PESTICIDES CHROMATIN-STRUCTURE-ASSAY MESSENGER-RNA-LEVELS CELL-APOPTOSIS IN-VITRO AROMATIC-HYDROCARBONS ESTROGENIC-EXPOSURE OXIDATIVE-STRESS M. Long A. Stronati D. Bizzaro T. Kruger P. S. Hjelmborg M. Spano A. Giwercman G. Toft J. P. Bonde E. C. Bonefeld Jorgensen MANICARDI, Gian Carlo Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
topic_facet |
POLYCHLORINATED-BIPHENYLS-PCBS EJACULATED-HUMAN-SPERMATOZOA CURRENTLY-USED-PESTICIDES CHROMATIN-STRUCTURE-ASSAY MESSENGER-RNA-LEVELS CELL-APOPTOSIS IN-VITRO AROMATIC-HYDROCARBONS ESTROGENIC-EXPOSURE OXIDATIVE-STRESS |
description |
Persistent organic pollutants (POPs) can interfere with hormone activities and are suspected as endocrine disrupters involved in disorders, e.g. reproductive disorders. We investigated the possible relation between the actual integrated serum xenoestrogenic, xenoandrogenic and aryl hydrocarbon receptor (AhR) activities, and the sperm DNA damage and sperm apoptotic markers of 262 adult males (54 Inuits from Greenland, 69 from Warsaw (Poland), 81 from Sweden, and 58 from Kharkiv (Ukraine)) exposed to different levels of POPs. Xenobiotic-induced receptor activities were determined by receptor-mediated luciferase reporter gene expression. Sperm DNA damage was measured using terminal deoxynucleotidyl transferase-driven dUTP nick labeling assay (TUNEL) and pro- (Fas) and anti-apoptotic (Bcl-xL) markers were determined by immune methods. Different features of xenobiotic-induced receptor activity in serum and sperm DNA fragmentation and apoptotic markers existed between the Inuits and the European Caucasians. Negative correlations between xenobiotic-induced receptor activities and DNA damage were found for Inuits having relatively lower xenoestrogenic, lower dioxin-like activity, and lower sperm DNA damage, but higher xenoandrogenic activity. In contrast, in the European groups, xenobiotic-induced receptor activities were found to be positively correlated with the DNA damage. Further research must elucidate whether altered receptor activities in concerted action with genetic and/or nutrient factors may have protecting effect on sperm DNA damage of the Inuit population. |
author2 |
M., Long A., Stronati D., Bizzaro T., Kruger Manicardi, Gian Carlo P. S., Hjelmborg M., Spano A., Giwercman G., Toft J. P., Bonde E. C., Bonefeld Jorgensen |
format |
Article in Journal/Newspaper |
author |
M. Long A. Stronati D. Bizzaro T. Kruger P. S. Hjelmborg M. Spano A. Giwercman G. Toft J. P. Bonde E. C. Bonefeld Jorgensen MANICARDI, Gian Carlo |
author_facet |
M. Long A. Stronati D. Bizzaro T. Kruger P. S. Hjelmborg M. Spano A. Giwercman G. Toft J. P. Bonde E. C. Bonefeld Jorgensen MANICARDI, Gian Carlo |
author_sort |
M. Long |
title |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_short |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_full |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_fullStr |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_full_unstemmed |
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations |
title_sort |
relation between serum xenobiotic-induced receptor activities and sperm dna damage and sperm apoptotic markers in european and inuit populations |
publishDate |
2007 |
url |
http://hdl.handle.net/11380/310064 https://doi.org/10.1530/REP-06-0195 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland inuit inuits |
genre_facet |
Greenland inuit inuits |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/17307920 info:eu-repo/semantics/altIdentifier/wos/WOS:000244680500018 volume:133 issue:2 firstpage:517 lastpage:530 journal:REPRODUCTION http://hdl.handle.net/11380/310064 doi:10.1530/REP-06-0195 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33847675040 |
op_doi |
https://doi.org/10.1530/REP-06-0195 |
container_title |
Reproduction |
container_volume |
133 |
container_issue |
2 |
container_start_page |
517 |
op_container_end_page |
530 |
_version_ |
1790601014173237248 |