Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica
Five psychrotolerant Alcanivorax spp. strains were isolated from Antarctic coastal waters. Strains were screened for molecular and physiological properties and analyzed regarding their growth capacity. Partial 16S rDNA, alk-B1, and P450 gene sequencing was performed. Biolog EcoPlates and the API 20E...
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Online Access: | https://hdl.handle.net/11380/1314107 https://doi.org/10.3390/microorganisms11010058 |
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ftunivmodena:oai:iris.unimore.it:11380/1314107 2024-04-21T07:50:28+00:00 Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica Cappello, Simone Corsi, Ilaria Patania, Sabrina Bergami, Elisa Azzaro, Maurizio Mancuso, Monique Genovese, Maria Lunetta, Alessia Caruso, Gabriella Cappello, Simone Corsi, Ilaria Patania, Sabrina Bergami, Elisa Azzaro, Maurizio Mancuso, Monique Genovese, Maria Lunetta, Alessia Caruso, Gabriella 2022 https://hdl.handle.net/11380/1314107 https://doi.org/10.3390/microorganisms11010058 eng eng info:eu-repo/semantics/altIdentifier/pmid/36677350 info:eu-repo/semantics/altIdentifier/wos/WOS:000918901500001 volume:11 issue:1 firstpage:1 lastpage:14 journal:MICROORGANISMS https://hdl.handle.net/11380/1314107 doi:10.3390/microorganisms11010058 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85146772222 info:eu-repo/semantics/openAccess Alcanivorax spp Antarctica biodegradation molecular analyse physiological analyses info:eu-repo/semantics/article 2022 ftunivmodena https://doi.org/10.3390/microorganisms11010058 2024-03-28T01:16:00Z Five psychrotolerant Alcanivorax spp. strains were isolated from Antarctic coastal waters. Strains were screened for molecular and physiological properties and analyzed regarding their growth capacity. Partial 16S rDNA, alk-B1, and P450 gene sequencing was performed. Biolog EcoPlates and the API 20E test were used to evaluate metabolic and biochemical profiles. Bacterial growth in sodium acetate was determined at 4, 15, 20, and 25 degrees C to evaluate the optimal temperature. Furthermore, the ability of each strain to grow in a hydrocarbon mixture at 4 and 25 degrees C was assayed. Biosurfactant production tests (drop-collapse and oil spreading) and emulsification activity tests (E-24) were also performed. Concerning results of partial gene sequencing (16S rDNA, alk-B1, and P450), a high similarity of the isolates with the same genes isolated from other Alcanivorax spp. strains was observed. The metabolic profiles obtained by Biolog assays showed no significant differences in the isolates compared to the Alcanivorax borkumensis wild type. The results of biodegradative tests showed their capability to grow at different temperatures. All strains showed biosurfactant production and emulsification activity. Our findings underline the importance to proceed in the isolation and characterization of Antarctic hydrocarbon-degrading bacterial strains since their biotechnological and environmental applications could be useful even for pollution remediation in polar areas. Article in Journal/Newspaper Antarc* Antarctic Antarctica Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) Microorganisms 11 1 58 |
institution |
Open Polar |
collection |
Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
op_collection_id |
ftunivmodena |
language |
English |
topic |
Alcanivorax spp Antarctica biodegradation molecular analyse physiological analyses |
spellingShingle |
Alcanivorax spp Antarctica biodegradation molecular analyse physiological analyses Cappello, Simone Corsi, Ilaria Patania, Sabrina Bergami, Elisa Azzaro, Maurizio Mancuso, Monique Genovese, Maria Lunetta, Alessia Caruso, Gabriella Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica |
topic_facet |
Alcanivorax spp Antarctica biodegradation molecular analyse physiological analyses |
description |
Five psychrotolerant Alcanivorax spp. strains were isolated from Antarctic coastal waters. Strains were screened for molecular and physiological properties and analyzed regarding their growth capacity. Partial 16S rDNA, alk-B1, and P450 gene sequencing was performed. Biolog EcoPlates and the API 20E test were used to evaluate metabolic and biochemical profiles. Bacterial growth in sodium acetate was determined at 4, 15, 20, and 25 degrees C to evaluate the optimal temperature. Furthermore, the ability of each strain to grow in a hydrocarbon mixture at 4 and 25 degrees C was assayed. Biosurfactant production tests (drop-collapse and oil spreading) and emulsification activity tests (E-24) were also performed. Concerning results of partial gene sequencing (16S rDNA, alk-B1, and P450), a high similarity of the isolates with the same genes isolated from other Alcanivorax spp. strains was observed. The metabolic profiles obtained by Biolog assays showed no significant differences in the isolates compared to the Alcanivorax borkumensis wild type. The results of biodegradative tests showed their capability to grow at different temperatures. All strains showed biosurfactant production and emulsification activity. Our findings underline the importance to proceed in the isolation and characterization of Antarctic hydrocarbon-degrading bacterial strains since their biotechnological and environmental applications could be useful even for pollution remediation in polar areas. |
author2 |
Cappello, Simone Corsi, Ilaria Patania, Sabrina Bergami, Elisa Azzaro, Maurizio Mancuso, Monique Genovese, Maria Lunetta, Alessia Caruso, Gabriella |
format |
Article in Journal/Newspaper |
author |
Cappello, Simone Corsi, Ilaria Patania, Sabrina Bergami, Elisa Azzaro, Maurizio Mancuso, Monique Genovese, Maria Lunetta, Alessia Caruso, Gabriella |
author_facet |
Cappello, Simone Corsi, Ilaria Patania, Sabrina Bergami, Elisa Azzaro, Maurizio Mancuso, Monique Genovese, Maria Lunetta, Alessia Caruso, Gabriella |
author_sort |
Cappello, Simone |
title |
Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica |
title_short |
Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica |
title_full |
Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica |
title_fullStr |
Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica |
title_full_unstemmed |
Characterization of Five Psychrotolerant Alcanivorax spp. Strains Isolated from Antarctica |
title_sort |
characterization of five psychrotolerant alcanivorax spp. strains isolated from antarctica |
publishDate |
2022 |
url |
https://hdl.handle.net/11380/1314107 https://doi.org/10.3390/microorganisms11010058 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/36677350 info:eu-repo/semantics/altIdentifier/wos/WOS:000918901500001 volume:11 issue:1 firstpage:1 lastpage:14 journal:MICROORGANISMS https://hdl.handle.net/11380/1314107 doi:10.3390/microorganisms11010058 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85146772222 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3390/microorganisms11010058 |
container_title |
Microorganisms |
container_volume |
11 |
container_issue |
1 |
container_start_page |
58 |
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1796934187719065600 |