Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters
The production of Reactive Oxygen Species (ROS) during desiccation processes is documented in bacteria and plants, whereas studies on animals are in practice lacking. In this study we investigated the response to dehydration with respect to the ROS production during the kinetic of the desiccation pr...
Main Authors: | , , |
---|---|
Other Authors: | , , , |
Format: | Conference Object |
Language: | English |
Published: |
Bourel et al
2014
|
Subjects: | |
Online Access: | http://hdl.handle.net/11380/1063108 |
id |
ftunivmodena:oai:iris.unimore.it:11380/1063108 |
---|---|
record_format |
openpolar |
spelling |
ftunivmodena:oai:iris.unimore.it:11380/1063108 2024-01-14T10:11:30+01:00 Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters GIOVANNINI, ILARIA GUIDETTI, Roberto REBECCHI, Lorena Bourel Giovannini, Ilaria Guidetti, Roberto Rebecchi, Lorena 2014 STAMPA http://hdl.handle.net/11380/1063108 eng eng Bourel et al country:FRA place:Pornichet ispartofbook:New Frontiers in anhydrobiosis New Frontiers in anhydrobiosis volume:1 firstpage:84 lastpage:84 http://hdl.handle.net/11380/1063108 tardigrada ROS storage cells info:eu-repo/semantics/conferenceObject 2014 ftunivmodena 2023-12-19T23:23:54Z The production of Reactive Oxygen Species (ROS) during desiccation processes is documented in bacteria and plants, whereas studies on animals are in practice lacking. In this study we investigated the response to dehydration with respect to the ROS production during the kinetic of the desiccation process and the short and long-time permanence in the anhydrobiotic state. We hypothesized that ROS production, and thus oxidative damage, may be greater in animals maintained desiccated for long-time than in animals maintained dry just for one day. The tardigrade Paramacrobiotus richtersi was used as a target animal. In parallel, oxidative stress was assessed in hydrated P. richtersi as control. Adults were experimentally dehydrated in laboratory using an optimal desiccation protocol to achieve a 100% survival rate of P. richtersi. Animals were maintained desiccated (at 3% RH and 20°C) from 1 to 40 days. Intracellular ROS production during rehydration was evaluated in the “storage cells” (free-floating cells in the tardigrade body cavity), after treatment of the tardigrades with the probe 2,7-dichlorodihydrofluorescein-diacetate (DCFH2-DA). The amount of green fluorescent oxidation product (DCF), which reflects the reaction of the probe with intracellular free radicals, was measure by a laser scanner confocal microscope. The desiccation process does not produce a high amount of ROS, even though previous studies on the same species, P. richtersi, demonstrated an increase of antioxidant enzyme activity in desiccated specimens with respect to hydrated ones. Instead, the long-time permanence in anhydrobiosis (20 days) produces a significant increase of ROS, as evidenced after three and twelve hours from rehydration. The collection of data about the permanence in anhydrobiosis for 40 days is in progress. The high amount of ROS detected in animals maintained dry for long-time, and consequently the oxidative molecular damages, justifies that tardigrade survival decreases with the time spent in a dry state. Conference Object Tardigrade water bear Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
institution |
Open Polar |
collection |
Archivio della ricerca dell'Università di Modena e Reggio Emilia (Unimore: IRIS) |
op_collection_id |
ftunivmodena |
language |
English |
topic |
tardigrada ROS storage cells |
spellingShingle |
tardigrada ROS storage cells GIOVANNINI, ILARIA GUIDETTI, Roberto REBECCHI, Lorena Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters |
topic_facet |
tardigrada ROS storage cells |
description |
The production of Reactive Oxygen Species (ROS) during desiccation processes is documented in bacteria and plants, whereas studies on animals are in practice lacking. In this study we investigated the response to dehydration with respect to the ROS production during the kinetic of the desiccation process and the short and long-time permanence in the anhydrobiotic state. We hypothesized that ROS production, and thus oxidative damage, may be greater in animals maintained desiccated for long-time than in animals maintained dry just for one day. The tardigrade Paramacrobiotus richtersi was used as a target animal. In parallel, oxidative stress was assessed in hydrated P. richtersi as control. Adults were experimentally dehydrated in laboratory using an optimal desiccation protocol to achieve a 100% survival rate of P. richtersi. Animals were maintained desiccated (at 3% RH and 20°C) from 1 to 40 days. Intracellular ROS production during rehydration was evaluated in the “storage cells” (free-floating cells in the tardigrade body cavity), after treatment of the tardigrades with the probe 2,7-dichlorodihydrofluorescein-diacetate (DCFH2-DA). The amount of green fluorescent oxidation product (DCF), which reflects the reaction of the probe with intracellular free radicals, was measure by a laser scanner confocal microscope. The desiccation process does not produce a high amount of ROS, even though previous studies on the same species, P. richtersi, demonstrated an increase of antioxidant enzyme activity in desiccated specimens with respect to hydrated ones. Instead, the long-time permanence in anhydrobiosis (20 days) produces a significant increase of ROS, as evidenced after three and twelve hours from rehydration. The collection of data about the permanence in anhydrobiosis for 40 days is in progress. The high amount of ROS detected in animals maintained dry for long-time, and consequently the oxidative molecular damages, justifies that tardigrade survival decreases with the time spent in a dry state. |
author2 |
Bourel Giovannini, Ilaria Guidetti, Roberto Rebecchi, Lorena |
format |
Conference Object |
author |
GIOVANNINI, ILARIA GUIDETTI, Roberto REBECCHI, Lorena |
author_facet |
GIOVANNINI, ILARIA GUIDETTI, Roberto REBECCHI, Lorena |
author_sort |
GIOVANNINI, ILARIA |
title |
Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters |
title_short |
Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters |
title_full |
Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters |
title_fullStr |
Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters |
title_full_unstemmed |
Desiccation tolerance and production of Reactive Oxygen Species (ROS) in the anhydrobiotic water bear Paramacrobiotus richters |
title_sort |
desiccation tolerance and production of reactive oxygen species (ros) in the anhydrobiotic water bear paramacrobiotus richters |
publisher |
Bourel et al |
publishDate |
2014 |
url |
http://hdl.handle.net/11380/1063108 |
genre |
Tardigrade water bear |
genre_facet |
Tardigrade water bear |
op_relation |
ispartofbook:New Frontiers in anhydrobiosis New Frontiers in anhydrobiosis volume:1 firstpage:84 lastpage:84 http://hdl.handle.net/11380/1063108 |
_version_ |
1788066409774841856 |