Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies
The shift of farmed Atlantic salmon from a marine- to plant-based diet the last decades has revealed challenges concerning the dietary requirements necessary to ensure fish health. Of the nutrients lacking in plant feeds, choline has been identified as essential to guarantee efficient lipid transpor...
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Norwegian University of Life Sciences, Ås
2024
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Online Access: | https://hdl.handle.net/11250/3126789 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/3126789 2024-05-19T07:37:27+00:00 Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies Lipid- og kolintilførsel påvirker steatose i enterocytter hos atlantisk laks : in vivo og in vitro studier Siciliani, Daphne Kortner, Trond M. Dhanasiri, Anusha K. S. Nyquist, Nicole Frost 2024 application/pdf https://hdl.handle.net/11250/3126789 eng eng Norwegian University of Life Sciences, Ås PhD thesis;2024:10 urn:isbn:978-82-575-2127-1 urn:issn:1894-6402 https://hdl.handle.net/11250/3126789 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no Atlantic salmon Lipid metabolism Choline requirement DNA methylation In vitro intestinal models Intestinal lipid accumulation Doctoral thesis 2024 ftunivmob 2024-04-24T00:02:14Z The shift of farmed Atlantic salmon from a marine- to plant-based diet the last decades has revealed challenges concerning the dietary requirements necessary to ensure fish health. Of the nutrients lacking in plant feeds, choline has been identified as essential to guarantee efficient lipid transport and metabolism. The distinctive sign of choline deficiency is an excessive presence of unabsorbed fat accumulating within the intestinal enterocytes, a condition known as steatosis. The results of the first attempts to define choline requirement for post-smolt Atlantic salmon were published recently. However, it can be expected that choline requirements vary with life stages and environmental and dietary conditions, but information on such relationships is lacking. Besides being fundamental for lipid transport, choline has gained particular interest due to its important role in DNA methylation processes. So far, very little is known regarding the effects of variation in DNA methylation in fish, but it is likely that disturbances in methylation processes may lead to increased disease risk. In this thesis, two studies, resulting in three papers, were conducted to investigate how choline requirement in farmed Atlantic salmon may be influenced by different production conditions, and how dietary choline level may interfere with DNA methylation. However, our understanding of the cellular mechanisms responsible for nutrient interactions with fish digestive physiology is still fragmentary. To address this, the third study in this thesis focused on in vitro studies of lipid metabolism. This approach is an important tool to facilitate further research into the basic functions of the digestive tract. In the first study, we investigated the effects of increasing dietary lipid levels on steatosis symptoms. This study involved Atlantic salmon parr that were fed choline-deficient diets. The fish were raised at two different environmental temperatures: 8 and 15 °C. The results showed that fish raised at 15 °C had higher feed intake ... Doctoral or Postdoctoral Thesis Atlantic salmon Open archive Norwegian University of Life Sciences: Brage NMBU |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
topic |
Atlantic salmon Lipid metabolism Choline requirement DNA methylation In vitro intestinal models Intestinal lipid accumulation |
spellingShingle |
Atlantic salmon Lipid metabolism Choline requirement DNA methylation In vitro intestinal models Intestinal lipid accumulation Siciliani, Daphne Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
topic_facet |
Atlantic salmon Lipid metabolism Choline requirement DNA methylation In vitro intestinal models Intestinal lipid accumulation |
description |
The shift of farmed Atlantic salmon from a marine- to plant-based diet the last decades has revealed challenges concerning the dietary requirements necessary to ensure fish health. Of the nutrients lacking in plant feeds, choline has been identified as essential to guarantee efficient lipid transport and metabolism. The distinctive sign of choline deficiency is an excessive presence of unabsorbed fat accumulating within the intestinal enterocytes, a condition known as steatosis. The results of the first attempts to define choline requirement for post-smolt Atlantic salmon were published recently. However, it can be expected that choline requirements vary with life stages and environmental and dietary conditions, but information on such relationships is lacking. Besides being fundamental for lipid transport, choline has gained particular interest due to its important role in DNA methylation processes. So far, very little is known regarding the effects of variation in DNA methylation in fish, but it is likely that disturbances in methylation processes may lead to increased disease risk. In this thesis, two studies, resulting in three papers, were conducted to investigate how choline requirement in farmed Atlantic salmon may be influenced by different production conditions, and how dietary choline level may interfere with DNA methylation. However, our understanding of the cellular mechanisms responsible for nutrient interactions with fish digestive physiology is still fragmentary. To address this, the third study in this thesis focused on in vitro studies of lipid metabolism. This approach is an important tool to facilitate further research into the basic functions of the digestive tract. In the first study, we investigated the effects of increasing dietary lipid levels on steatosis symptoms. This study involved Atlantic salmon parr that were fed choline-deficient diets. The fish were raised at two different environmental temperatures: 8 and 15 °C. The results showed that fish raised at 15 °C had higher feed intake ... |
author2 |
Kortner, Trond M. Dhanasiri, Anusha K. S. Nyquist, Nicole Frost |
format |
Doctoral or Postdoctoral Thesis |
author |
Siciliani, Daphne |
author_facet |
Siciliani, Daphne |
author_sort |
Siciliani, Daphne |
title |
Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
title_short |
Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
title_full |
Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
title_fullStr |
Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
title_full_unstemmed |
Lipid and choline supply affect Atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
title_sort |
lipid and choline supply affect atlantic salmon enterocyte steatosis : in vivo and in vitro studies |
publisher |
Norwegian University of Life Sciences, Ås |
publishDate |
2024 |
url |
https://hdl.handle.net/11250/3126789 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
PhD thesis;2024:10 urn:isbn:978-82-575-2127-1 urn:issn:1894-6402 https://hdl.handle.net/11250/3126789 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
_version_ |
1799476748479365120 |