Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx
Gill disease is a major threat to aquaculture of Atlantic salmon, with an unknown and likely underestimated contribution from cnidarians such as jellyfish and biofouling hydroids. To better understand the risk and thus enable mitigation, technology for the certain identification of cnidarian-related...
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Online Access: | https://hdl.handle.net/11250/3126650 https://doi.org/10.1007/s10499-024-01482-8 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/3126650 2024-05-19T07:37:41+00:00 Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx Bloecher, Nina Østevik, Liv Floerl, Oliver Sivertsgård, Rolf Aas, Marianne Kvæstad, Bjarne Ribičić, Deni Netzer, Roman 2024-04-15T12:29:53Z application/pdf https://hdl.handle.net/11250/3126650 https://doi.org/10.1007/s10499-024-01482-8 eng eng Egen institusjon: SINTEF Ocean urn:issn:0967-6120 https://hdl.handle.net/11250/3126650 https://doi.org/10.1007/s10499-024-01482-8 cristin:2261648 Aquaculture International VDP::Fiskerifag: 920 VDP::Fisheries science: 920 Peer reviewed Journal article 2024 ftunivmob https://doi.org/10.1007/s10499-024-01482-8 2024-04-24T00:02:14Z Gill disease is a major threat to aquaculture of Atlantic salmon, with an unknown and likely underestimated contribution from cnidarians such as jellyfish and biofouling hydroids. To better understand the risk and thus enable mitigation, technology for the certain identification of cnidarian-related gill damage is needed. We used the hydroid Ectopleura larynx in a case study to determine whether the exposure of salmon to nematocyst-bearing hydrozoans can be deducted via non-destructive PCR-based methods. In a field experiment, we evaluated (i) whether swabbing the inside of the gill operculum in farmed Salmo salar and subsequent PCR analysis can provide quantifiable information about the presence of E. larynx material in the gill chamber and, if so, (ii) whether the screening results correlate with histological assessments of gill damage. The developed PCR methods were able to detect the presence of biofouling hydroids in ambient water. However, despite exposure to suspended hydroid particle concentrations that did result in gill damage in some salmon, quantitative PCR results did not correlate with histological gill assessments. For opercular swabs to serve as a diagnostic tool for detecting biofouling-mediated gill damage in live salmon, increased specificity of genetic markers and improved sampling methods are needed. Animal trial permit no. 24252 (granted 06.07.2020, Norwegian Food Safety Authority). Gill disease · Atlantic salmon · Salmo salar · Biofouling · Net cleaning publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU Aquaculture International |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
topic |
VDP::Fiskerifag: 920 VDP::Fisheries science: 920 |
spellingShingle |
VDP::Fiskerifag: 920 VDP::Fisheries science: 920 Bloecher, Nina Østevik, Liv Floerl, Oliver Sivertsgård, Rolf Aas, Marianne Kvæstad, Bjarne Ribičić, Deni Netzer, Roman Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx |
topic_facet |
VDP::Fiskerifag: 920 VDP::Fisheries science: 920 |
description |
Gill disease is a major threat to aquaculture of Atlantic salmon, with an unknown and likely underestimated contribution from cnidarians such as jellyfish and biofouling hydroids. To better understand the risk and thus enable mitigation, technology for the certain identification of cnidarian-related gill damage is needed. We used the hydroid Ectopleura larynx in a case study to determine whether the exposure of salmon to nematocyst-bearing hydrozoans can be deducted via non-destructive PCR-based methods. In a field experiment, we evaluated (i) whether swabbing the inside of the gill operculum in farmed Salmo salar and subsequent PCR analysis can provide quantifiable information about the presence of E. larynx material in the gill chamber and, if so, (ii) whether the screening results correlate with histological assessments of gill damage. The developed PCR methods were able to detect the presence of biofouling hydroids in ambient water. However, despite exposure to suspended hydroid particle concentrations that did result in gill damage in some salmon, quantitative PCR results did not correlate with histological gill assessments. For opercular swabs to serve as a diagnostic tool for detecting biofouling-mediated gill damage in live salmon, increased specificity of genetic markers and improved sampling methods are needed. Animal trial permit no. 24252 (granted 06.07.2020, Norwegian Food Safety Authority). Gill disease · Atlantic salmon · Salmo salar · Biofouling · Net cleaning publishedVersion |
format |
Article in Journal/Newspaper |
author |
Bloecher, Nina Østevik, Liv Floerl, Oliver Sivertsgård, Rolf Aas, Marianne Kvæstad, Bjarne Ribičić, Deni Netzer, Roman |
author_facet |
Bloecher, Nina Østevik, Liv Floerl, Oliver Sivertsgård, Rolf Aas, Marianne Kvæstad, Bjarne Ribičić, Deni Netzer, Roman |
author_sort |
Bloecher, Nina |
title |
Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx |
title_short |
Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx |
title_full |
Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx |
title_fullStr |
Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx |
title_full_unstemmed |
Evaluation of novel PCR‑based method to assess gill injuries in fish caused by the cnidarian Ectopleura larynx |
title_sort |
evaluation of novel pcr‑based method to assess gill injuries in fish caused by the cnidarian ectopleura larynx |
publishDate |
2024 |
url |
https://hdl.handle.net/11250/3126650 https://doi.org/10.1007/s10499-024-01482-8 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquaculture International |
op_relation |
Egen institusjon: SINTEF Ocean urn:issn:0967-6120 https://hdl.handle.net/11250/3126650 https://doi.org/10.1007/s10499-024-01482-8 cristin:2261648 |
op_doi |
https://doi.org/10.1007/s10499-024-01482-8 |
container_title |
Aquaculture International |
_version_ |
1799477023317426176 |