Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay
Viral diseases are among of the main challenges in aquaculture in Norway today. Heart- and skeletal muscle inflammation, caused by Piscine orthoreovirus (PRV), and pancreas disease (PD), caused by Salmonid alphavirus (SAV), both cause huge losses of farmed Atlantic salmon. Vaccination is a possible...
Main Author: | |
---|---|
Other Authors: | , , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
Published: |
Norwegian University of Life Sciences, Ås
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/3053470 |
id |
ftunivmob:oai:nmbu.brage.unit.no:11250/3053470 |
---|---|
record_format |
openpolar |
spelling |
ftunivmob:oai:nmbu.brage.unit.no:11250/3053470 2023-05-15T15:30:26+02:00 Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay Deteksjon av antistoffer i atlantisk laks med et kulebasert multipleks immunoassay Teige, Lena Hammerlund Boysen, Preben Dahle, Maria K. Storset, Anne 2020 application/pdf https://hdl.handle.net/11250/3053470 eng eng Norwegian University of Life Sciences, Ås PhD Thesis;2020:01 Norges forskningsråd: 237315/E40 ViVaFish 280847/E40 ViVaAct urn:isbn:978-82-575-1665-9 urn:issn:1894-6402 https://hdl.handle.net/11250/3053470 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND Atlantic salmon Piscine orthoreovirus Salmonid alphavirus IgM Immunoassay Antibody Doctoral thesis 2020 ftunivmob 2023-03-01T23:45:19Z Viral diseases are among of the main challenges in aquaculture in Norway today. Heart- and skeletal muscle inflammation, caused by Piscine orthoreovirus (PRV), and pancreas disease (PD), caused by Salmonid alphavirus (SAV), both cause huge losses of farmed Atlantic salmon. Vaccination is a possible solution that is already contributing to the control of bacterial diseases. Unfortunately, making effective vaccines against viral diseases has been challenging and information about which immune mechanisms that are involved in protection is lacking. Antibodies can mediate complete protection against some diseases, and antibody levels after vaccination can correlate with protection, even if the protection is mediated by other mechanisms. In addition, antibody detection can be used in disease surveillance to determine if a fish population is or has been infected with a virus. Unfortunately, measuring antibody levels in salmon is not straightforward. Such measurements have not been widely used, and often show high levels of background binding. An important reason for this is that fish antibodies are of the IgM type. IgM is less specific than IgG, the dominating antibody in mammals. Therefore, other methods, like PCR and histology, are more used in diagnostics in Atlantic salmon. In this work, we have for the first time used an assay based on microscopic magnetic beads conjugated with antigen to measure antibody levels in Atlantic salmon. This method has a high sensitivity and can be used to measure antibodies against several proteins simultaneously in the same sample. By using this method, we have detected antibodies against PRV and SAV. In paper I and II, plasma from two PRV challenge trials was used to detect antibodies against the PRV proteins μ1c, μNS and σ1. We also detected antibodies against PRV1- σ1 in plasma from PRV-3-infected rainbow trout. There have been no previous publications detecting antibodies against PRV. The peak antibody level coincided with decreased pathology in the heart. In addition to showing ... Doctoral or Postdoctoral Thesis Atlantic salmon Open archive Norwegian University of Life Sciences: Brage NMBU Norway Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
topic |
Atlantic salmon Piscine orthoreovirus Salmonid alphavirus IgM Immunoassay Antibody |
spellingShingle |
Atlantic salmon Piscine orthoreovirus Salmonid alphavirus IgM Immunoassay Antibody Teige, Lena Hammerlund Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay |
topic_facet |
Atlantic salmon Piscine orthoreovirus Salmonid alphavirus IgM Immunoassay Antibody |
description |
Viral diseases are among of the main challenges in aquaculture in Norway today. Heart- and skeletal muscle inflammation, caused by Piscine orthoreovirus (PRV), and pancreas disease (PD), caused by Salmonid alphavirus (SAV), both cause huge losses of farmed Atlantic salmon. Vaccination is a possible solution that is already contributing to the control of bacterial diseases. Unfortunately, making effective vaccines against viral diseases has been challenging and information about which immune mechanisms that are involved in protection is lacking. Antibodies can mediate complete protection against some diseases, and antibody levels after vaccination can correlate with protection, even if the protection is mediated by other mechanisms. In addition, antibody detection can be used in disease surveillance to determine if a fish population is or has been infected with a virus. Unfortunately, measuring antibody levels in salmon is not straightforward. Such measurements have not been widely used, and often show high levels of background binding. An important reason for this is that fish antibodies are of the IgM type. IgM is less specific than IgG, the dominating antibody in mammals. Therefore, other methods, like PCR and histology, are more used in diagnostics in Atlantic salmon. In this work, we have for the first time used an assay based on microscopic magnetic beads conjugated with antigen to measure antibody levels in Atlantic salmon. This method has a high sensitivity and can be used to measure antibodies against several proteins simultaneously in the same sample. By using this method, we have detected antibodies against PRV and SAV. In paper I and II, plasma from two PRV challenge trials was used to detect antibodies against the PRV proteins μ1c, μNS and σ1. We also detected antibodies against PRV1- σ1 in plasma from PRV-3-infected rainbow trout. There have been no previous publications detecting antibodies against PRV. The peak antibody level coincided with decreased pathology in the heart. In addition to showing ... |
author2 |
Boysen, Preben Dahle, Maria K. Storset, Anne |
format |
Doctoral or Postdoctoral Thesis |
author |
Teige, Lena Hammerlund |
author_facet |
Teige, Lena Hammerlund |
author_sort |
Teige, Lena Hammerlund |
title |
Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay |
title_short |
Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay |
title_full |
Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay |
title_fullStr |
Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay |
title_full_unstemmed |
Detection of antibodies in Atlantic salmon using a bead-based multiplexed immunoassay |
title_sort |
detection of antibodies in atlantic salmon using a bead-based multiplexed immunoassay |
publisher |
Norwegian University of Life Sciences, Ås |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/3053470 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Norway Sav’ |
geographic_facet |
Norway Sav’ |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
PhD Thesis;2020:01 Norges forskningsråd: 237315/E40 ViVaFish 280847/E40 ViVaAct urn:isbn:978-82-575-1665-9 urn:issn:1894-6402 https://hdl.handle.net/11250/3053470 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766360876964642816 |