Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line
Infectious salmon anemia (ISA) is a bothersome epidemy in commercially farmed Atlantic salmon (Salmo salar), caused by the influenza-like Infectious salmon anemia virus (ISAV). It causes severe anemia in farmed Atlantic salmon, resulting in high death rates and great economical losses if left untrea...
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Norwegian University of Life Sciences, Ås
2022
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Online Access: | https://hdl.handle.net/11250/3046168 |
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ftunivmob:oai:nmbu.brage.unit.no:11250/3046168 2023-05-15T15:28:43+02:00 Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line Myhre, Aurora Mula Sandvik, Guro 2022 application/pdf https://hdl.handle.net/11250/3046168 eng eng Norwegian University of Life Sciences, Ås https://hdl.handle.net/11250/3046168 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND Master thesis 2022 ftunivmob 2023-02-01T23:44:37Z Infectious salmon anemia (ISA) is a bothersome epidemy in commercially farmed Atlantic salmon (Salmo salar), caused by the influenza-like Infectious salmon anemia virus (ISAV). It causes severe anemia in farmed Atlantic salmon, resulting in high death rates and great economical losses if left untreated. The infectious disease has demanded a lot of resources in the science and aquaculture communities since its discovery. Many countermeasures, such as infection control guidelines and vaccines, have been tried out, but with varying result in the field. To this day ISAV outbreaks are still common in most counties commercially farming Atlantic salmon, though not as frequent and severe as before. Previous research on the salmon immune system indicates that interferons play an important role in the salmons first line of defense against virus infections. Salmon has a great repertoire of such interferons and interferon-like genes, that have been studied to varying degree. These interferons and interferon-like genes are therefore of great interest in the search for better virus immune response in farmed Atlantic salmon, both for ISA resistance and for virus resistance in general. The aim of this thesis was to shed some light on what genes contribute to the natural immune response of Atlantic salmon. Specifically, which, if any, interferons or interferon-like genes contribute to ISA virus resistance. To achieve this, it will be attempted to utilize the high sequence similarity of Atlantic salmon interferons and interferon-like genes to design a CRISPR knockout experiment, intended to knock out multiple high-similarity genes. submittedVersion M-BIOTEK Master Thesis Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU |
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Open archive Norwegian University of Life Sciences: Brage NMBU |
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ftunivmob |
language |
English |
description |
Infectious salmon anemia (ISA) is a bothersome epidemy in commercially farmed Atlantic salmon (Salmo salar), caused by the influenza-like Infectious salmon anemia virus (ISAV). It causes severe anemia in farmed Atlantic salmon, resulting in high death rates and great economical losses if left untreated. The infectious disease has demanded a lot of resources in the science and aquaculture communities since its discovery. Many countermeasures, such as infection control guidelines and vaccines, have been tried out, but with varying result in the field. To this day ISAV outbreaks are still common in most counties commercially farming Atlantic salmon, though not as frequent and severe as before. Previous research on the salmon immune system indicates that interferons play an important role in the salmons first line of defense against virus infections. Salmon has a great repertoire of such interferons and interferon-like genes, that have been studied to varying degree. These interferons and interferon-like genes are therefore of great interest in the search for better virus immune response in farmed Atlantic salmon, both for ISA resistance and for virus resistance in general. The aim of this thesis was to shed some light on what genes contribute to the natural immune response of Atlantic salmon. Specifically, which, if any, interferons or interferon-like genes contribute to ISA virus resistance. To achieve this, it will be attempted to utilize the high sequence similarity of Atlantic salmon interferons and interferon-like genes to design a CRISPR knockout experiment, intended to knock out multiple high-similarity genes. submittedVersion M-BIOTEK |
author2 |
Sandvik, Guro |
format |
Master Thesis |
author |
Myhre, Aurora Mula |
spellingShingle |
Myhre, Aurora Mula Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line |
author_facet |
Myhre, Aurora Mula |
author_sort |
Myhre, Aurora Mula |
title |
Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line |
title_short |
Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line |
title_full |
Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line |
title_fullStr |
Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line |
title_full_unstemmed |
Mini CRISPR screen of Interferons and interferon-like genes in search of ISAV resistance in SHK-1 cell line |
title_sort |
mini crispr screen of interferons and interferon-like genes in search of isav resistance in shk-1 cell line |
publisher |
Norwegian University of Life Sciences, Ås |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3046168 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://hdl.handle.net/11250/3046168 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766359052463374336 |