Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart
Farmed salmon suffer from a variety of heart pathologies that impair cardiac function and ultimately contribute to cardiac failure and mortality. Thyroid hormones (TH) have well-studied cardioprotective effects in mammals, through direct, genomic and non-genomic (acute) actions. TH enhance cardiac a...
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Norwegian University of Life Sciences, Ås
2022
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ftunivmob:oai:nmbu.brage.unit.no:11250/3021723 2023-05-15T15:31:29+02:00 Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart Spanos, Georgios Frisk, Michael Vindas, Marco Hodne, Kjetil Johansen, Ida Beitnes 2022 application/pdf https://hdl.handle.net/11250/3021723 eng eng Norwegian University of Life Sciences, Ås https://hdl.handle.net/11250/3021723 Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no CC-BY-NC-ND Atlantic salmon Ca+2 cycling Thyroid hormones heart cardiomyocyte VDP::Agriculture and fishery disciplines: 900 VDP::Medical disciplines: 700 Master thesis 2022 ftunivmob 2022-09-28T22:43:09Z Farmed salmon suffer from a variety of heart pathologies that impair cardiac function and ultimately contribute to cardiac failure and mortality. Thyroid hormones (TH) have well-studied cardioprotective effects in mammals, through direct, genomic and non-genomic (acute) actions. TH enhance cardiac activity and promote physiological cardiac remodeling while they are an important regulator of Ca+2 transients of the cardiomyocyte. TH peak during critical events of salmon life cycle with increased cardiac demand (e.g., smoltification), but their role in cardiac physiology in Atlantic salmon (Salmo salar) remain largely unknown. The aim of the current study was to investigate whether TH have direct acute and genomic effects on Ca+2 dynamics in the salmon heart. To investigate acute effects, salmon ventricular cardiomyocytes were isolated using a standard enzymatic technique, while Ca+2 transients were recorded in the presence of different concentrations of the TH, 3,3’,5-Triiodo-L-thyroinine (T3). To investigate genomic effects, whole hearts were incubated under different concentration of T3 and the expression of genes encoding proteins related to Ca+2 cycling and hypertrophy was quantified by qPCR. Stimulation with T3 acutely decreased the time of half removal and increased the Ca+2 extrusion rate of the Ca+2 transient. These findings are largely in line with the observed acute effects of T3 on mammalian cardiomyocytes and could help the heart to work effectively when the heart rate is elevated. Further investigation is required to understand the underlying physiological mechanisms. The study did not reveal any genomic effects of TH on the salmon heart. Although these findings partially agree with previous findings in other teleost fish but limiting factors of our study do not allow us to exclude genomic effects of TH on the salmon heart. Nevertheless, the results indicate that TH could have a cardioprotective role also in salmon, for example in contexts that increase cardiac demand, such as during smoltification. ... Master Thesis Atlantic salmon Salmo salar Open archive Norwegian University of Life Sciences: Brage NMBU |
institution |
Open Polar |
collection |
Open archive Norwegian University of Life Sciences: Brage NMBU |
op_collection_id |
ftunivmob |
language |
English |
topic |
Atlantic salmon Ca+2 cycling Thyroid hormones heart cardiomyocyte VDP::Agriculture and fishery disciplines: 900 VDP::Medical disciplines: 700 |
spellingShingle |
Atlantic salmon Ca+2 cycling Thyroid hormones heart cardiomyocyte VDP::Agriculture and fishery disciplines: 900 VDP::Medical disciplines: 700 Spanos, Georgios Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart |
topic_facet |
Atlantic salmon Ca+2 cycling Thyroid hormones heart cardiomyocyte VDP::Agriculture and fishery disciplines: 900 VDP::Medical disciplines: 700 |
description |
Farmed salmon suffer from a variety of heart pathologies that impair cardiac function and ultimately contribute to cardiac failure and mortality. Thyroid hormones (TH) have well-studied cardioprotective effects in mammals, through direct, genomic and non-genomic (acute) actions. TH enhance cardiac activity and promote physiological cardiac remodeling while they are an important regulator of Ca+2 transients of the cardiomyocyte. TH peak during critical events of salmon life cycle with increased cardiac demand (e.g., smoltification), but their role in cardiac physiology in Atlantic salmon (Salmo salar) remain largely unknown. The aim of the current study was to investigate whether TH have direct acute and genomic effects on Ca+2 dynamics in the salmon heart. To investigate acute effects, salmon ventricular cardiomyocytes were isolated using a standard enzymatic technique, while Ca+2 transients were recorded in the presence of different concentrations of the TH, 3,3’,5-Triiodo-L-thyroinine (T3). To investigate genomic effects, whole hearts were incubated under different concentration of T3 and the expression of genes encoding proteins related to Ca+2 cycling and hypertrophy was quantified by qPCR. Stimulation with T3 acutely decreased the time of half removal and increased the Ca+2 extrusion rate of the Ca+2 transient. These findings are largely in line with the observed acute effects of T3 on mammalian cardiomyocytes and could help the heart to work effectively when the heart rate is elevated. Further investigation is required to understand the underlying physiological mechanisms. The study did not reveal any genomic effects of TH on the salmon heart. Although these findings partially agree with previous findings in other teleost fish but limiting factors of our study do not allow us to exclude genomic effects of TH on the salmon heart. Nevertheless, the results indicate that TH could have a cardioprotective role also in salmon, for example in contexts that increase cardiac demand, such as during smoltification. ... |
author2 |
Frisk, Michael Vindas, Marco Hodne, Kjetil Johansen, Ida Beitnes |
format |
Master Thesis |
author |
Spanos, Georgios |
author_facet |
Spanos, Georgios |
author_sort |
Spanos, Georgios |
title |
Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart |
title_short |
Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart |
title_full |
Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart |
title_fullStr |
Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart |
title_full_unstemmed |
Acute and genomic effects of Thyroid Hormones on the Atlantic salmon (Salmo salar) heart |
title_sort |
acute and genomic effects of thyroid hormones on the atlantic salmon (salmo salar) heart |
publisher |
Norwegian University of Life Sciences, Ås |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3021723 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://hdl.handle.net/11250/3021723 |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no |
op_rightsnorm |
CC-BY-NC-ND |
_version_ |
1766362000815816704 |